JCRB9085 P3X63Ag8U.1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9085 Cell Name   P3X63Ag8U.1
Profile   myeloma, plasmacytoma, 8-azaguanine resistant, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   myeloma derivative
Life Span    Crisis PDL   
Morphology    Character   myeloma derivative
Classify    Established by   Kohler & Milstein
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL1597. Year   1985
Medium   DMEM + 4.5g/L glucose + NEAA + FCS 10% Methods for Passages   Dilution. A density of 1-1.5x10^6/ml should not be exceeded.
Cell Number on Passage   3-5 x 10^5/ml Race   
CO2 Conc.   5 % Tissue Sampling   plasmacytoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3412
Reference
Pubmed id:3500068 Reciprocal effects of epidermal growth factor and transforming growth factor beta on the anchorage-dependent and -independent growth of A431 epidermoid carcinoma cells.
Lee K,Tanaka M,Hatanaka M,Kuze F
Exp Cell Res. 1987 Nov;173(1):156-62
Pubmed id:113458 A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
Kearney JF,Radbruch A,Liesegang B,Rajewsky K
J Immunol. 1979 Oct;123(4):1548-50
Pubmed id:567551 Fusion of mouse myeloma and spleen cells.
Yelton DE,Diamond BA,Kwan SP,Scharff MD
Curr Top Microbiol Immunol. 1978;81():1-7
Pubmed id:74805 Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse.
Lemke H,Hammerling GJ,Hohmann C,Rajewsky K
Nature. 1978 Jan 19;271(5642):249-51
Pubmed id:268647 Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.
Koprowski H,Gerhard W,Croce CM
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8
Pubmed id:1172191 Continuous cultures of fused cells secreting antibody of predefined specificity.
Köhler G,Milstein C
Nature. 1975 Aug 7;256(5517):495-7




LOT Information

Cell No.   JCRB9085 Cell Name   
LOT No.   03142002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose), non-essential amino acids, and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   D.T. = ca 18 hrs Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   Unknown (5 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   culture medium with 10% DMSO, 20% FBS CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9085 Cell Name   P3X63Ag8U.1
LOT No.   111688 Lot Specification   distribution
Medium   DMEM,4.5g/L glucose,non essen amino acids + FCS10% Temperature   37
Cell Density at Seeding   3-5 x 10^5/ml Methods for Passages   Dilution. A density of 1-1.5x10^6/ml should not be exceeded.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P4 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
CLOSE