JCRB9094 DLD-1

Cell information

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Cross-contamination of JCRB9094 DLD-1

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9094 Cell Name   DLD-1
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   colon
Case history    Metastasis   
Tissue Metastasized    Genetics   colon, adenocarcinoma
Life Span    Crisis PDL   
Morphology    Character   colon, adenocarcinoma
Classify    Established by   Dexter,D.L. et al.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL 221. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/2 - 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0248
Reference
Pubmed id:14720324Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma.
Onogawa S,Kitadai Y,Tanaka S,Kuwai T,Kimura S,Chayama K
Cancer Sci. 2004 Jan;95(1):32-9
Pubmed id:8643486Prostaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect.
Kutchera W,Jones DA,Matsunami N,Groden J,McIntyre TM,Zimmerman GA,White RL,Prescott SM
Proc Natl Acad Sci U S A. 1996 May 14;93(10):4816-20
Pubmed id:7621404DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.
Chen TR,Dorotinsky CS,McGuire LJ,Macy ML,Hay RJ
Cancer Genet Cytogenet. 1995 Jun;81(2):103-8
Pubmed id:8127905Nuclear matrix proteins in human colon cancer.
Keesee SK,Meneghini MD,Szaro RP,Wu YJ
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1913-6
Pubmed id:1699228p53 mutations in colorectal cancer.
Rodrigues NR,Rowan A,Smith ME,Kerr IB,Bodmer WF,Gannon JV,Lane DP
Proc Natl Acad Sci U S A. 1990 Oct;87(19):7555-9
Pubmed id:1980764Expression of several growth factors and their receptor genes in human colon carcinomas.
Ito M,Yoshida K,Kyo E,Ayhan A,Nakayama H,Yasui W,Ito H,Tahara E
Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(3):173-8
Pubmed id:3338874Biological characterization and oncogene expression in human colorectal carcinoma cell lines.
Trainer DL,Kline T,McCabe FL,Faucette LF,Feild J,Chaikin M,Anzano M,Rieman D,Hoffstein S,Li DJ
Int J Cancer. 1988 Feb 15;41(2):287-96
Pubmed id:6652615Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins.
Chen TR,Hay RJ,Macy ML
Cancer Genet Cytogenet. 1983 Dec;10(4):351-62
Pubmed id:427742N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells.
Dexter DL,Barbosa JA,Calabresi P
Cancer Res. 1979 Mar;39(3):1020-5

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LOT Information

Cell No.   JCRB9094 Cell Name   
LOT No.   04012010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (typs B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9094 Cell Name   
LOT No.   09082004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   31 hrs Cell Number in Vial (cells/1ml)   6.8 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9094 Cell Name   DLD-1
LOT No.   120695 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Flow 0070893) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 7 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.35x10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   P22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9094 Cell Name   DLD-1
LOT No.   062992 Lot Specification   distribution
Medium   RPMI1640 + 10% FBS (J.R.S. DO10400) Temperature   37 C
Cell Density at Seeding   3x10^6 Methods for Passages   Harvest with 0.02% EDTA and 0.25% trypsin.
Doubling Time   20hrs. Cell Number in Vial (cells/1ml)   2X10^6
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9094 Cell Name   DLD-1
LOT No.   111588 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP,G6PD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9094 Cell Name   
LOT No.   032798 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   24-28 hrs Cell Number in Vial (cells/1ml)   6.9 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9094 Cell Name   DLD-1
LOT No.   09152016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.7 - 2.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DSMO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.4
Additional information   
Cell No.   JCRB9094 Cell Name   DLD-1
LOT No.   07082021 Lot Specification   distribution
Medium   RPMI1640 medium(Wako:catalog No.189-02025) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   2.0-2.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.87x10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   p23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:8,11
D7S820:10,12
D16S539:12,13
VWA:18,19
TH01:7,9.3
AM:X,Y
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.4
Additional information   
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