JCRB9104 Detroit 510

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9104 Cell Name   Detroit 510
Profile   Human skin, galactosemia cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   skin, galactosemia
Life Span    Crisis PDL   
Morphology    Character   skin, galactosemia
Classify    Established by   Stulberg,C.S et al.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL 72. Population doubling is unknown. MEM contains non essential amino acids, sodium pyruvate, lactalbumin hydrolysate (0.1%). Year   1986
Medium   Eagle's minimal essential medium (see comment) with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.2% trypsin and disperse.
Cell Number on Passage   Dilute cells by 1/3. Race   
CO2 Conc.   5 % Tissue Sampling   skin
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2427
Reference
Pubmed id:5059566 Galactose utilization in galactosemia.
Petricciani JC,Binder MK,Merril CR,Geier MR
Science. 1972 Mar 24;175(4028):1368-70
Pubmed id:14294068 CONTACT INHIBITION, MACROMOLECULAR SYNTHESIS, AND POLYRIBOSOMES IN CULTURED HUMAN DIPLOID FIBROBLASTS.
LEVINE EM,BECKER Y,BOONE CW,EAGLE H
Proc Natl Acad Sci U S A. 1965 Feb;53():350-6
Pubmed id:13315373 Galactosemia, a congenital defect in a nucleotide transferase.
KALCKAR HM,ANDERSON EP,ISSELBACHER KJ
Biochim Biophys Acta. 1956 Apr;20(1):262-8

Images



LOT Information

Cell No.   JCRB9104 Cell Name   Detroit 510
LOT No.   072889 Lot Specification   distribution
Medium   MEM (see comment) + FCS 10% Temperature   37
Cell Density at Seeding   1/3 Methods for Passages   0.02% EDTA and 0.2% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.0x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
CLOSE