JCRB9110 PC-3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9110 Cell Name   PC-3
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   prostate gland
Case history    Metastasis   
Tissue Metastasized    Genetics   adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Kaighn,M.E.
Registered by    Regulation for Distribution   
Comment    Year   1987
Medium   Kaighn's modification (F12K) of Ham's F12 medium with 7% FBS Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   prostate
Virus Summary   tested Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested, GAPDH = positive control]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0035
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905934
Reference
Pubmed id:7471073 Chromosomal analysis of human prostatic adenocarcinoma cell lines.
Ohnuki Y,Marnell MM,Babcock MS,Lechner JF,Kaighn ME
Cancer Res. 1980 Mar;40(3):524-34


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Pubmed id:2112242 Isolation of human cDNA clones of jun-related genes, jun-B and jun-D.
Nomura N,Ide M,Sasamoto S,Matsui M,Date T,Ishizaki R
Nucleic Acids Res. 1990 May 25;18(10):3047-8

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LOT Information

Cell No.   JCRB9110 Cell Name   
LOT No.   06082011 Lot Specification   distribution
Medium   Ham's F12K medium with 7% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.6 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability (%)   90.8 Antibiotics Used   free
Passage Number   P46 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:11
D7S820:8,11
D16S539:11
VWA:17
TH01:6,7
AM:X
TPOX:8,9
CSF1PO:11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K CO2 Conc.   5%
RFLP    Additional information   
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Cell No.   JCRB9110 Cell Name   
LOT No.   08182008 Lot Specification   distribution
Medium   Kaighn's modification of Ham's F12 medium (F12K) with 7% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   3 -10 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.5 day Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability (%)   89.6 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - F12K CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB9110 Cell Name   PC-3
LOT No.   051192 Lot Specification   distribution
Medium   Ham F-12K (Flow Lab.lot#.0110142) + 7% FBS (Cell Culture Lab. lot#.00939-01) Temperature   37 C
Cell Density at Seeding   8x10^5 Methods for Passages   0.02 % EDTA + 0.25 % trypsin (floating cells were harvested together)
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.8x10^5
Viability (%)   96.1 Antibiotics Used   free
Passage Number   P23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB9110 Cell Name   
LOT No.   031298 Lot Specification   distribution
Medium   Ham's F12K medium with 7% fetal calf serum Ample mislabeled; JCRB9011 on ampule => JCRB9110 Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   30-35 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability (%)   98.0 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB9110 Cell Name   PC-3
LOT No.   01192016 Lot Specification   distribution
Medium   Kaighn's modification of Ham's F12 medium (F12K) with 7% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.2 - 9.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability (%)   97.1 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K medium CO2 Conc.   5%
RFLP    Additional information   
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