JCRB9111 BeWo

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9111 Cell Name   BeWo
Profile   Human placenta derived cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   placenta
Case history   chorio carcinoma Metastasis   
Tissue Metastasized    Genetics   trophoblastic cell line
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   the first human endocrine cell type cultivated
Classify    Established by   Pattillo,R.A. & Gey,G.O.
Registered by    Regulation for Distribution   Under the treatment with ATCC.
Comment   Prepared from CCL 98. Gender of this cell line is not described in the original paper. Riken cell bank informed us that the Y chromosome observed in this cell line. STR profile also suggested that this cell line is male origin. Year   1987
Medium   Ham's F12K medium with 15% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   6x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   fetal placenta
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0044
Reference
Pubmed id:6067662L-asparagine biosynthesis by nutritional variants of the Jensen sarcoma.
Patterson MK Jr,Orr G
Biochem Biophys Res Commun. 1967 Jan 23;26(2):228-33
Pubmed id:14403619Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens.
HSU TC,KELLOGG DS Jr
J Natl Cancer Inst. 1960 Aug;25():221-35

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LOT Information

Cell No.   JCRB9111 Cell Name   BeWo
LOT No.   122292 Lot Specification   distribution
Medium   Ham`s F12 medium (040892,121192) with 15% fetal bovine serum (Hyclone,1151031/JRHB,1B1062) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Cells were treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.08x10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P206 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9,11
D7S820:10,12
D16S539:13,14
VWA:16
TH01:9,9.3
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB9111 Cell Name   BeWo
LOT No.   100590 Lot Specification   distribution
Medium   Ham's F12(K) + FCS 15% Temperature   37
Cell Density at Seeding   1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.6x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P200 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9111 Cell Name   
LOT No.   05262000 Lot Specification   distribution
Medium   Ham's F12 medium with 15% fetal bovine serum (FBS lot; Bioscience 40039) Temperature   37 C
Cell Density at Seeding   0.5-1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 36 hrs Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   P202 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9111 Cell Name   BeWo
LOT No.   06272014 Lot Specification   distribution
Medium   Ham's F12K medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.9 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   71.5 Antibiotics Used   free
Passage Number   P213 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:9,11
D7S820:10,12
D16S539:13,14
VWA:16
TH01:9,9.3
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K medium CO2 Conc.   5%
Viability immediately after thawing (%)   71.5 Additional information   
Cell No.   JCRB9111 Cell Name   BeWo
LOT No.   09172019 Lot Specification   distribution
Medium   Ham's F12K (Kaighn's Modification of Ham's F-12: GIIBCO Cat. #21-127-022) with 15% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 57 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95-4 Antibiotics Used   free
Passage Number   P214 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K CO2 Conc.   5%
Viability immediately after thawing (%)   94.8 Additional information   
Cell No.   JCRB9111 Cell Name   BeWo
LOT No.   09262022 Lot Specification   distribution
Medium    Ham's F12K (Kaighn's Modification of Ham's F-12: GIIBCO Cat. #21-127-022) with 15% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   5.3 - 9.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   5.8 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P214 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:9,11
D7S820:10,12
D16S539:13,14
VWA:16
TH01:9,9.3
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - Ham's F12K CO2 Conc.   5%
Viability immediately after thawing (%)   70.0 Additional information   
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