JCRB9119 Ramos(RA1)

Cell information

Important Notice(s)

On the cultivation of JCRB9119 Ramos(RA-1)

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9119 Cell Name   Ramos(RA1)
Profile   lymphoma, Burkitt's (EBV negative) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   Burkitt lymphoma (EBV negative)
Life Span    Crisis PDL   
Morphology    Character   Burkitt lymphoma (EBV negative)
Classify   CRL1596 Established by   
Registered by   B.C. Giovalella Regulation for Distribution   
Comment    Year   1988
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage   2-4 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   Burkitt lymphoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0597
Reference
Pubmed id:14896 Tumorigenicity of human hematopoietic cell lines in athymic nude mice.
Nilsson K,Giovanella BC,Stehlin JS,Klein G
Int J Cancer. 1977 Mar 15;19(3):337-44
Pubmed id:196397 Heterogeneity of Epstein-Barr virus. II. Induction of early antigens (EA) by complementation.
zur Hausen H,Fresen KO
Virology. 1977 Aug;81(1):138-43
Pubmed id:181343

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Images



LOT Information

Cell No.   JCRB9119 Cell Name   Ramos(RA1)
LOT No.   06222012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Simple dilution (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   91.4 Antibiotics Used   free
Passage Number   P39 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9119 Cell Name   Ramos(RA1)
LOT No.   11172008 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.4-4.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.69x10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   p40 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:7,12
D13S317:13,14
D7S820:11
D16S539:10,13
VWA:15,16
TH01:7,9.3
AM:X
TPOX:8,9
CSF1PO:10,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9119 Cell Name   Ramos(RA1)
LOT No.   020194 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (JRS,DO10400) Temperature   37 C
Cell Density at Seeding   1-4x10^5 cells/ml Methods for Passages   dilution
Doubling Time   dt=24.2 hrs Cell Number in Vial (cells/1ml)   1.16x10^7
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   P38 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9119 Cell Name   Ramos(RA1)
LOT No.   121391 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   1.4x10^6 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   95% Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9119 Cell Name   Ramos(RA1)
LOT No.   050988 Lot Specification   distribution
Medium   RPMI1640 + 10 % FCS Temperature   37
Cell Density at Seeding   2-4x10^5/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P36 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9119 Cell Name   
LOT No.   052197 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.5-1.9 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   5.2 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9119 Cell Name   Ramos(RA1)
LOT No.   05112018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.6 - 4.4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 17 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   80.0 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80.0
Additional information   
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