-Detailed Information [JCRB9119]-

[On the cultivation of JCRB9119 Ramos(RA-1)]

Ramos(RA-1) seems wead for freeze-thawing, and many dead cells will be observed after thawing.

The survival of cells will be dependent on the cell density. Therefore please take the following procedure at the re-cultivation of cells from frozen vial.

1) Suspend the all vial contents to 10 mL culture medium containing FBS after thawing.
2) To remove DMSO, centrifuge the cell suspension at 1200 rpm for 5 min, and remove the supernatant.
3) Resuspend the cells to 5 mL of fresh culture medium with FBS.
4) Plate the all cell suspension to one well of 6 well plate.
5) Ramos cells will rapidly grow when they start the proliferation. To avoid the overgrowth, please prepare the additional culture by dilution (take 1 mL of cell suspension and suspend to 4 mL fresh merium; 1/5 dilution). Original culture should also be continued to cultivate.

[JCRB9119 Ramos(RA-1)の培養に関するお願い]

Ramos (RA-1)は凍結融解に弱い傾向があるとみられ、解凍後多数の死細胞が観察されます。

解凍後の生存性は細胞密度に依存しますので、次の手順をとってください。

1) バイアルを解凍後、10 mlの血清入り培地にサスペンド
2) 1200 rpm, 5分遠心し、上清を除く(DMSOを除くため)
3) 細胞を5 mLの血清入り培地に再浮遊
4) 6 ウェルプレートの1ウェルにて培養を開始
5) 増殖が開始されると増殖速度は速いため、解凍翌日、細胞の状態をみて、1 mLをとり、1/5希釈した培養も用意してください。


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JCRB No.	JCRB9119	Cell Name	Ramos(RA1)
Profile	lymphoma, Burkitt's (EBV negative)	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	hemo-lymphocytic
Case history		Metastasis
Tissue Metastasized		Genetics	Burkitt lymphoma (EBV negative)
Life Span		Crisis PDL
Morphology		Character	Burkitt lymphoma (EBV negative)
Classify	CRL1596	Established by
Registered by	B.C. Giovalella	Regulation for Distribution
Comment		Year	1988
Medium	RPMI1640 medium with 10% fetal calf serum.	Methods for Passages	dilution
Cell Number on Passage	2-4 x 10^5 cells/ml	Race
CO2 Conc.	5 %	Tissue Sampling	Burkitt lymphoma
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested	Detected RNA Virus	tested
CMV	-	parvoB19	-	HCV	-	HTLV-1	-
EBV	-	HBV	-	HIV-1	-	HTLV-2	-
HHV6	-	HTLV-1	-	HIV-2	-	HAV	-
HHV7	-	HTLV-2	-	-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
BKV	-	HIV-1	-
JCV	-	HIV-2	-
ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0597

Reference
Pubmed id:196397	Heterogeneity of Epstein-Barr virus. II. Induction of early antigens (EA) by complementation. zur Hausen H,Fresen KO Virology. 1977 Aug;81(1):138-43
Pubmed id:14896	Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Nilsson K,Giovanella BC,Stehlin JS,Klein G Int J Cancer. 1977 Mar 15;19(3):337-44
Pubmed id:181343	An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Klein G,Giovanella B,Westman A,Stehlin JS,Mumford D Intervirology. 1975;5(6):319-34

06222012
11172008
020194
121391
050988
052197
05112018
09042020

Cell No.	JCRB9119	Cell Name	Ramos(RA1)
LOT No.	06222012	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding		Methods for Passages	Simple dilution (suspension culture)
Doubling Time		Cell Number in Vial (cells/1ml)	2.6 x 10^6
Viability at cell freezing (%)	91.4	Antibiotics Used	free
Passage Number	P39	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9119	Cell Name	Ramos(RA1)
LOT No.	11172008	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911)	Temperature	37 C
Cell Density at Seeding	1.4-4.0x10^5 cells/ml	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.69x10^6
Viability at cell freezing (%)	96.7	Antibiotics Used	free
Passage Number	p40	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:7,12 D13S317:13,14 D7S820:11 D16S539:10,13 VWA:15,16 TH01:7,9.3 AM:X TPOX:8,9 CSF1PO:10,11
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB9119	Cell Name	Ramos(RA1)
LOT No.	020194	Lot Specification	distribution
Medium	RPMI-1640 medium with 10% fetal bovine serum (JRS,DO10400)	Temperature	37 C
Cell Density at Seeding	1-4x10^5 cells/ml	Methods for Passages	dilution
Doubling Time	dt=24.2 hrs	Cell Number in Vial (cells/1ml)	1.16x10^7
Viability at cell freezing (%)	94.2	Antibiotics Used	free
Passage Number	P38	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD, LDH, NP
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	No	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9119	Cell Name	Ramos(RA1)
LOT No.	121391	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37 C
Cell Density at Seeding	1.4x10^6	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.1x10^6
Viability at cell freezing (%)	95%	Antibiotics Used	free
Passage Number	P38	PDL
Sterility: MYCOPLASMA	NT	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	No	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9119	Cell Name	Ramos(RA1)
LOT No.	050988	Lot Specification	distribution
Medium	RPMI1640 + 10 % FCS	Temperature	37
Cell Density at Seeding	2-4x10^5/ml	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.1x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P36	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	LD
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9119	Cell Name
LOT No.	052197	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS lot BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	1.5-1.9 x 10^5 cells/ml	Methods for Passages	Dilution
Doubling Time		Cell Number in Vial (cells/1ml)	5.2 x 10^6
Viability at cell freezing (%)	94.0	Antibiotics Used	free
Passage Number	P38	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9119	Cell Name	Ramos(RA1)
LOT No.	05112018	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012).	Temperature	37 C
Cell Density at Seeding	1.6 - 4.4 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	approx. 17 hrs.	Cell Number in Vial (cells/1ml)	2.8 x 10^6
Viability at cell freezing (%)	80.0	Antibiotics Used	free
Passage Number	P38	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	80.0	Additional information

Cell No.	JCRB9119	Cell Name	Ramos(RA1)
LOT No.	09042020	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.1 - 2.5 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	14 - 18 hrs.	Cell Number in Vial (cells/1ml)	2.8 x 10^6
Viability at cell freezing (%)	97.3	Antibiotics Used	free
Passage Number	P38	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	96.5	Additional information

JCRB Cell Bank

JCRB9119 Ramos(RA1)

Cell information

LOT Information