JCRB9127 COS-7
Cell information
Important Notice(s)COS-7について（JCRB9127とIFO50068の二つの番号）
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9127 | Cell Name | COS-7 |
|---|---|---|---|
| Profile | African green monkey cell line producing T antigen of SV40. | Other Name | |
| Animal | monkey, African green | Strain | |
| Genus | Cercopithecus | Species | aethiops |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | CV-1 cells transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. | Metastasis | |
| Tissue Metastasized | Genetics | Producing T antigen of SV40. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Supports ts A209 virus growth at 40C & SV40 having deletions in the early region. |
| Classify | transformed | Established by | Gluzman,Y. |
| Registered by | JCRB | Regulation for Distribution | |
| Comment | Year | 1997 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (corrected on 2002.1.16). | Methods for Passages | Cells are treated with EDTA and Trypsin. |
| Cell Number on Passage | 2.5 x 10^4 cells/sq.cm. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:6260373 | SV40-transformed simian cells support the replication of early SV40 mutants. Gluzman Y Cell. 1981 Jan;23(1):175-82 |
| Research results by users. Click！ | |
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| Pubmed id:31847265 |
Applicability of Anti-Human Estrogen Receptor β Antibody PPZ0506 for the Immunodetection of Rodent Estrogen Receptor β Proteins. Ishii H,Otsuka M,Kanaya M,Higo S,Hattori Y,Ozawa H Int J Mol Sci. 2019 Dec 13;20(24): |
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LOT Information
| Cell No. | JCRB9127 | Cell Name | COS-7 |
|---|---|---|---|
| LOT No. | 07122010 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 1.02-4.3x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.10x10^6 |
| Viability at cell freezing (%) | 91.1 | Antibiotics Used | free |
| Passage Number | p9* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB9127 | Cell Name | |
|---|---|---|---|
| LOT No. | 10052007 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% FBS (FBS Lot GIBCO 484375) | Temperature | 37 C |
| Cell Density at Seeding | approx. 5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 30 hrs | Cell Number in Vial (cells/1ml) | 2.1 x 10^6 |
| Viability at cell freezing (%) | 87 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as African green monkey by NP, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9127 | Cell Name | |
|---|---|---|---|
| LOT No. | 11222004 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 2-6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | ca. 30 hrs | Cell Number in Vial (cells/1ml) | 6.0 x 10^5 |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | P8* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as African green monkey by NP, G6PD, MD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9127 | Cell Name | COS-7 |
|---|---|---|---|
| LOT No. | 031497 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (Mitsubishi, lot.PVF01). | Temperature | 37 C |
| Cell Density at Seeding | 2-2.5x10^4 cells/sq.cm. | Methods for Passages | Cells harvested with 0.25% trypsin and 0.02% EDTA for 5 min. and diluted. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4x10^6 |
| Viability at cell freezing (%) | 97.0 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB9127 | Cell Name | |
|---|---|---|---|
| LOT No. | 02292000 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0122) | Temperature | 37 C |
| Cell Density at Seeding | 5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | D.T.=ca 34 hrs | Cell Number in Vial (cells/1ml) | 7.0 x 10^5 |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | P8* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as African green monkey by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9127 | Cell Name | COS-7 |
|---|---|---|---|
| LOT No. | 02072014 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 2.6 - 4.5 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 | |
| Viability at cell freezing (%) | 98.5 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as African green monkey by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 98.5 | Additional information |