JCRB9128 Mv 1 Lu

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9128 Cell Name   Mv 1 Lu
Profile   useful for focus forming assays of murine and feline sarcoma virus Other Name   NBL-7
Animal   mink Strain   Aleutian
Genus   Mustela Species   vison
Sex    Age   fetus
Identity    Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   This cell line is useful for focus forming assays of murine and feline sarcoma virus.
Classify   normal Established by   Kniazeff,A.J.,Nelson-Rees,W.A.,& Darby,Jr.N.B.
Registered by    Regulation for Distribution   
Comment    Year   1997
Medium   Eagle's minimal essential medium with non-essential amino acids, and Earle's BSS with reduced bicarbonate (0.85g/L) and 10% newborn calf serum. Methods for Passages   Cells are harvested by treatment with 0.25% trypsin and 0.02% EDTA for 5 min at room temperature.
Cell Number on Passage   2.2x10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0593
Reference
Pubmed id:4366800 Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses.
Henderson IC,Lieber MM,Todaro GJ
Virology. 1974 Jul;60(1):282-7

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LOT Information

Cell No.   JCRB9128 Cell Name   Mv 1 Lu
LOT No.   051697 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 0.85g/L bicarbonate, NEAA and 10% fetal calf serum/Bioscience Temperature   37 C
Cell Density at Seeding   2.2x10^4 cells/cm^2 Methods for Passages   Cells harvested with 0.25% trypsin and 0.02% EDTA for 5 min. at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   P46 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, LDH examined. Mink (NP*).
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB9128 Cell Name   Mv 1 Lu
LOT No.   01162017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.3 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   16 - 54 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P50 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.0
Additional information   
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