JCRB9130 DT40

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9130 Cell Name   DT40
Profile   avian leukosis virus (ALV)-induced lymphomas Other Name   
Animal   chicken Strain   Hyline SC
Genus   avian Species   
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   lymphoma Metastasis   
Tissue Metastasized    Genetics   c-myc+
Life Span   infinite Crisis PDL   NT
Morphology   lymphoblast-like Character   Immunoglobulin IgM (surface) production.
Classify   tumor Established by   Humphries,E.H.
Registered by    Regulation for Distribution   none
Comment    Year   1997
Medium   Dulbecco's modified Eagle's medium with 2mM L-glutamine & 0.05mM 2-mer.EtOH, tryptose phosphate broth, 10%;FBS, 10%; chicken serum, 5%. Methods for Passages   Simple dilution every 2 - 3 days.
Cell Number on Passage   3 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   bursa lymphoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0249
Reference
Pubmed id:8254742v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel.
Hrdlicková R,Nehyba J,Humphries EH
J Virol. 1994 Jan;68(1):308-19
Pubmed id:1490388V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas.
Humphries EH,Zhang G
Curr Top Microbiol Immunol. 1992;182():475-83
Pubmed id:2998040Cell lines derived from avian lymphomas exhibit two distinct phenotypes.
Baba TW,Giroir BP,Humphries EH
Virology. 1985 Jul 15;144(1):139-51

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LOT Information

Cell No.   JCRB9130 Cell Name   DT40
LOT No.   11172010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 4mM Glutamine,50uM　2-Mercaptoetanol,10% heat inactivated fetal bovine serum(SIGMA027K03911) and 1% Chicken serum(Invitrogen8165076) Temperature   39.5
Cell Density at Seeding   2-5x10^5 cells/ml Methods for Passages   dilution(1/10-1/20)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   74.4 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB9130 Cell Name   
LOT No.   02272006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 0.05 mM mercaptoethanol, 10% tryptose phosphate broth, 10% FBS and 5% chick serum Temperature   37 C
Cell Density at Seeding   2-6 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   Unknown (P5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as chick by NP, G6PD, AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No. Exoteric Gene   
Medium for Freezing   culture medium containing 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9130 Cell Name   DT40
LOT No.   081997 Lot Specification   distribution
Medium   DMEM, 10% tryptose phosphate broth, 0.05mM 2mercapto EtOH, 2mM L-glutamine, 10% FBS,5% Chickin serum Temperature   37 C
Cell Density at Seeding   2.8 x10^5 cells/ml Methods for Passages   Simple dilution once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.9x10^6
Viability at cell freezing (%)   73.0 Antibiotics Used   free
Passage Number   P2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Under examination.
Chromosome Mode   NT Chromosome Information   Under examination.
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
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