| Profile:Human liver bile duct carcinoma cell line. |
| Date accepted: | 01/20/1989 |
| Animal: | human |
| Scientific Name: | Homo sapiens |
| Classification: | new |
| Tissue prepared: | liver bile duct |
| Medium: | RPMI1640 medium with 10% fetal bovine serum. |
| Passage method: | Cells treated with 0.05 % trypsin and 0.02 % EDTA for 10 min at 37 C. |
| CO2 Concentration: | 5 % |
| Cell no. at passage: | split ratio= 1/4 |
| Genetics: | bile duct carcinoma |
| Characteristics: | bile duct carcinoma |
| Cell ID data: | available |
| DNA Profile (STR): | D5S818:12,13 D13S317:11,13 D7S820:10,11 D16S539:11,12 VWA:18 TH01:7,10 Amelogenin:X,Y TPOX:8 CSF1PO:11,12 |
| Virus DNA detected: | CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description |
| Established by | Miyagiwa,K. et al. |
| Deposited by | Sato,J.,Tokiwa,T. |
| Cell bank: | OUCI(JCRB) |
| References: | (1) |
(* The format of the table was revised on Apr.25.2003.)
| Cells(1), Mycoplasma test, Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process, |
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Additional Information(1) Additional Information(2) Additional Information(3) |
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[ Lot No.: 022196: seed (JCRB0425) ] |
Passage Number, P78 Cell culture was started without cloning from the previous stock. Date cell culture started:02/08/96 Concentration of cells in an ampule: 1.8x10^6 cell/ml Viability under microscope (%): 92.8 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, NP, LD, G6PD (type B of human). Anchorage dependency: Yes Cell Identification: available [ Culture condition of this lot.]
Freezing medium: Culture medium with 5% DMSO. Passage method: Cells treated with 0.25% trypsin and 0.02% EDTA with PBS(-). Growth temperature: 37 C Cell No. at passage: 4-7x10^4 cells/ml (T75) Memo: From lot.091994 by Satoh,M. in IFO. [ Additional Data ]
(Date downloaded: 2010-11-22) |
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