| ES medium(*7) | mg/liter | mg/liter | ||
| Amino Acids | Salts(*9) | |||
| l-Alanine | 18 | CaCl2 | 200 | |
| l-Arginine | 105 | Ca(NO3)2.4H2O | - | |
| l-Asparagine | 30(H2O) | CuSO4.5H2O | - | |
| l-Aspartic acid | 27 | FeSO4.7H2O | - | |
| l-Cysteine | - | Fe(NO3)3.9H2O | - | |
| l-Cystine | 24 | KCL | 400 | |
| l-Glutamic acid | 30 | KNO3 | - | |
| l-Glutamine | 292 | MgCl2 | - | |
| Glutathione, reduced | - | MgSO4 | 93.5 | |
| Glycine | 15 | MnCl2.4H2O | - | |
| l-Histidine | 31 | NaCl | 6,800 | |
| l-Hydroxyproline | - | NaHCO3 | - | |
| l-Isoleucine | 52 | Na2HPO4 | - | |
| l-Leucine | 52 | NaH2PO4 | 115 | |
| l-Lysine | 58 | SeO3 | - | |
| l-Methione | 15 | ZnSO4.7H2O | - | |
| l-Ornithine | - | Miscellaneous | ||
| l-Phenylalanine | 32 | Adenine.HCl | - | |
| l-Proline | 23 | Galactose | - | |
| l-Serine | 21 | Glucose(Dextrose) | 1,000 | |
| l-Threonine | 48 | Hepes | - | |
| l-Tryptophan | 10 | Hypoxanthine | - | |
| l-Tyrosine | 36 | Linoleic acid | - | |
| l-Valine | 46 | Lipoic acid | - | |
| Vitamins | Orotic acid | - | ||
| p-Aminobenzoic acid | - | Phenolsulfonphthalein | 6 | |
| Ascorbic acid(C) | - | Putrecine dihydrochloride | - | |
| Biotin(H) | 0.02 | Sodium pyruvate | 110 | |
| Choline | 1.8($5) | Thymidine | - | |
| Cyanocobalamin(B12) | 0.1 | |||
| Folic acid(M) | 1 | |||
| Inositol | 2 | |||
| Nicotinamide | 1 | |||
| Pantothenic acid | 1($1) | |||
| Pyridoxal(B6) | 1 | |||
| Pyrodoxine(B6) | - | |||
| Riboflavin(B2,G) | 0.1 | |||
| Thiamine(B1) | 1 |