| RPMI1640 | mg/liter | mg/liter | ||
| Amino Acids | Salts(*9) | |||
| l-Alanine | - | CaCl2 | - | |
| l-Arginine | 200 | Ca(NO3)2.4H2O | 100 | |
| l-Asparagine | 50 | CuSO4.5H2O | - | |
| l-Aspartic acid | 20 | FeSO4.7H2O | - | |
| l-Cysteine | - | Fe(NO3)3.9H2O | - | |
| l-Cystine | 50 | KCL | 400 | |
| l-Glutamic acid | 20 | KNO3 | - | |
| l-Glutamine | 300 | MgCl2 | - | |
| Glutathione, reduced | 1 | MgSO4 | 100 | |
| Glycine | 10 | MnCl2.4H2O | - | |
| l-Histidine | 15 | NaCl | 6,000 | |
| l-Hydroxyproline | 20 | NaHCO3 | 2,000 | |
| l-Isoleucine | 50 | Na2HPO4 | 1,512(7H2O) | |
| l-Leucine | 50 | NaH2PO4 | - | |
| l-Lysine | 40(HCl) | SeO3 | - | |
| l-Methione | 15 | ZnSO4.7H2O | - | |
| l-Ornithine | - | Miscellaneous | ||
| l-Phenylalanine | 15 | Adenine.HCl | - | |
| l-Proline | 20 | Galactose | - | |
| l-Serine | 30 | Glucose(Dextrose) | 2,000 | |
| l-Threonine | 20 | Hepes | - | |
| l-Tryptophan | 5 | Hypoxanthine | - | |
| l-Tyrosine | 20 | Linoleic acid | - | |
| l-Valine | 20 | Lipoic acid | - | |
| Vitamins | Orotic acid | - | ||
| p-Aminobenzoic acid | 1 | Phenolsulfonphthalein | 5 | |
| Ascorbic acid(C) | - | Putrecine dihydrochloride | - | |
| Biotin(H) | 0.2 | Sodium pyruvate | - | |
| Choline | 3(Cl) | Thymidine | - | |
| Cyanocobalamin(B12) | 0.005 | |||
| Folic acid(M) | 1 | |||
| Inositol | 35 | |||
| Nicotinamide | 1 | |||
| Pantothenic acid | 0.25(Ca) | |||
| Pyridoxal(B6) | - | |||
| Pyrodoxine(B6) | 1(HCl) | |||
| Riboflavin(B2,G) | 0.2 | |||
| Thiamine(B1) | 1(HCl) |