JCRB2208 A9(Neo8)

細胞情報

Important Notice(s)

ヒト単一染色体含有ライブラリ A9シリーズ細胞株の培養法

Information for the timing of selection for A9-series cell lines

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB2208 細胞名   A9(Neo8)
生物種(日本語)   ヒト染色体導入株 組織名(日本語)   単染色体導入パネル株
コメント(日本語)   ヒト 8番染色体導入パネル細胞 プロフィール   contains human chromosome # 8
別名   A9 動物名   human chromosome-introduced
系統名   C3H 学名・属名   Mus
種名   musclus 性別   
年齢・月齢    細胞識別情報   
(癌)原発組織名   human monochromosomal hybrid panel 病歴情報   
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質   98.7% cells included intact human chromosome #8 at deposit. 細胞寿命   infinite
クライシスPDL    形態   fibroblast-like
一般性状   skin fibroblast, AG-r. Cells include human chromosome #8. 細胞分類   panel
細胞樹立者名   Oshimura,M. 細胞寄託者   Oshimura,M.
分譲時制限    コメント   Human chromosome was selected by G418.
入手年   1997 培養培地   Dulbecco's modified Eagle's medium with 10% calf serum and 0.8mg/ml G418(Gibco,79N9555).
継代方法   Cells are treated with 0.25% trypsin. 継代時細胞数   2 x 10^4 cells/ml
人種    炭酸ガス濃度   5 %
採取組織名   skin ウィルス検査   
検出ウィルス    組織型   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy)はneXtProtプロジェクトの一環として、SIB - Swiss Institute of BioinformaticsのCALIPHOグループのAmos Bairoch博士によって開発されました。Cellosaurusは細胞株に関する様々な情報を集約させたデータベースになっています。JCRB細胞バンクに登録されている細胞株については、右のリンクから情報を得ることができます。 CVCL_2852
Reference
Pubmed id:10919723 Human monochromosome hybrid cell panel characterized by FISH in the JCRB/HSRRB.
Tanabe H,Nakagawa Y,Minegishi D,Hashimoto K,Tanaka N,Oshimura M,Sofuni T,Mizusawa H
Chromosome Res. 2000;8(4):319-34
Pubmed id:8527393 Normal human chromosome 2 induces cellular senescence in the human cervical carcinoma cell line SiHa.
Uejima H,Mitsuya K,Kugoh H,Horikawa I,Oshimura M
Genes Chromosomes Cancer. 1995 Oct;14(2):120-7
Pubmed id:7493906 Restoration of the cellular senescence program and repression of telomerase by human chromosome 3.
Ohmura H,Tahara H,Suzuki M,Ide T,Shimizu M,Yoshida MA,Tahara E,Shay JW,Barrett JC,Oshimura M
Jpn J Cancer Res. 1995 Oct;86(10):899-904
Pubmed id:8851764 Cosmids and transcribed sequences from chromosome 11q23.
Katoh M,Nakagawa Y,Yawata T,Kumano S,Kobayashi E,Kurimasa A,Kugoh H,Oshimura M
Jpn J Hum Genet. 1995 Dec;40(4):307-17
Pubmed id:1639397 Ordering of human chromosome 3p markers by radiation hybrid mapping.
Tamari M,Hamaguchi M,Shimizu M,Oshimura M,Takayama H,Kohno T,Yamaguchi N,Sugimura T,Terada M,Yokota J
Genomics. 1992 Jul;13(3):705-12
Pubmed id:1723716 Human complement regulatory proteins expressed on mouse A9 cells containing a human chromosome 1.
Seya T,Okada M,Hara T,Matsumoto M,Miyagawa S,Oshimura M
Immunology. 1991 Dec;74(4):719-24
Pubmed id:1995301 Introduction of new genetic markers on human chromosomes.
Satoh H,Barrett JC,Oshimura M
Exp Cell Res. 1991 Mar;193(1):5-11
Pubmed id:2267132 Suggestive evidence for functionally distinct, tumor-suppressor genes on chromosomes 1 and 11 for a human fibrosarcoma cell line, HT1080.
Kugoh HM,Hashiba H,Shimizu M,Oshimura M
Oncogene. 1990 Nov;5(11):1637-44
Pubmed id:1978855 Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the chromosomal assignment of radiosensitivity gene.
Ejima Y,Oshimura M,Sasaki MS
Int J Radiat Biol. 1990 Dec;58(6):989-97
Pubmed id:1702413 Normal human chromosome 1 carries suppressor activity for various phenotypes of a Kirsten murine sarcoma virus-transformed NIH/3T3 cell line.
Yamada H,Horikawa I,Hashiba H,Oshimura M
Jpn J Cancer Res. 1990 Nov;81(11):1095-100
Pubmed id:2392323 Multiple chromosomes carrying tumor suppressor activity for a uterine endometrial carcinoma cell line identified by microcell-mediated chromosome transfer.
Yamada H,Wake N,Fujimoto S,Barrett JC,Oshimura M
Oncogene. 1990 Aug;5(8):1141-7
Pubmed id:2502516 Construction of mouse A9 clones containing a single human chromosome tagged with neomycin-resistance gene via microcell fusion.
Koi M,Shimizu M,Morita H,Yamada H,Oshimura M
Jpn J Cancer Res. 1989 May;80(5):413-8
Pubmed id:2498246 Construction of mouse A9 clones containing a single human chromosome (X/autosome translocation) via micro-cell fusion.
Koi M,Morita H,Shimizu M,Oshimura M
Jpn J Cancer Res. 1989 Feb;80(2):122-5
Pubmed id:14213660 THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS.
LITTLEFIELD JW
Nature. 1964 Sep 12;203():1142-4

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LOT Information

細胞番号   JCRB2208 細胞名   A9(Neo8)
培養ロット番号   02012016 培養種別   distribution
培地   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010) and 0.8 mg/ml geneticin (G418; GIBCO Cat. # 10131). Geneticin should not be added until cells start to grow. 培養温度   37 C
継代時細胞数(濃度)   8.3 - 9.9 x 10^4 cells/mL 継代方法   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
増殖速度   31 - 34 hrs. 凍結時生細胞濃度   1.7 x 10^6
凍結直後生細胞率   88.8 使用抗生物質   free
継代数   P6 PDL数(プライマリ)   
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード    染色体情報   
表面抗原    DNA Profile (STR)   
接着性   Yes 導入外部遺伝子   
凍結培地   10% DMSO, 20% NCS - DMEM 炭酸ガス濃度   5%
RFPL所見    追加情報   
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