JCRB0125 L alpha

細胞情報

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB0125 細胞名   L alpha
生物種(日本語)   マウス 組織名(日本語)   皮膚
コメント(日本語)   L細胞のポリオウイルス受容体蛋白α遺伝子導入株, thymidine kinase欠損 プロフィール   L cell line introduced with poliovirus receptor protein alpha gene, thymidine kinase defective
別名    動物名   mouse
系統名    学名・属名   Mus
種名   musclus 性別   F
年齢・月齢    細胞識別情報   
(癌)原発組織名   skin 病歴情報   cells introduced by polio receptor gene.Cells contaminated by Mycoplasma but decontaminated in JCRB.
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質   Mouse L tk- cell line was transformed by cDNA of poliovirus receptor protein alpha. 細胞寿命   infinite
クライシスPDL    形態   epithelial-like
一般性状   This cell line expresses poliovirus receptor and sensitive to the poliovirus. 細胞分類   transformed
細胞樹立者名   Nomoto,A. 細胞寄託者   Nomoto,A.
分譲時制限   free コメント   Receptors are examined after decontaminaion of mycoplasmas.
入手年   1997 培養培地   Dulbecco's modified Eagle's medium with 5% fetal calf serum. (Mitsubishi PVF01 used as serum)).
継代方法   Cells are harvested with 0.125% trypsin and 0.02% EDTA at room temperature for 5 min. 継代時細胞数   1.5x10^4 cells/sq.cm.
人種    炭酸ガス濃度   5 %
採取組織名   loose connective tissue ウィルス検査   
検出ウィルス    組織型   
Reference
Pubmed id:7474095 A new cis-acting element for RNA replication within the 5' noncoding region of poliovirus type 1 RNA.
Shiroki K,Ishii T,Aoki T,Kobashi M,Ohka S,Nomoto A
J Virol. 1995 Nov;69(11):6825-32
Pubmed id:8389915 Temperature-sensitive mouse cell factors for strand-specific initiation of poliovirus RNA synthesis.
Shiroki K,Kato H,Koike S,Odaka T,Nomoto A
J Virol. 1993 Jul;67(7):3989-96
Pubmed id:1331508 A second gene for the African green monkey poliovirus receptor that has no putative N-glycosylation site in the functional N-terminal immunoglobulin-like domain.
Koike S,Ise I,Sato Y,Yonekawa H,Gotoh O,Nomoto A
J Virol. 1992 Dec;66(12):7059-66
Pubmed id:1851992 Functional domains of the poliovirus receptor.
Koike S,Ise I,Nomoto A
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4104-8
Pubmed id:2170108 The poliovirus receptor protein is produced both as membrane-bound and secreted forms.
Koike S,Horie H,Ise I,Okitsu A,Yoshida M,Iizuka N,Takeuchi K,Takegami T,Nomoto A
EMBO J. 1990 Oct;9(10):3217-24

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LOT Information

細胞番号   JCRB0125 細胞名   L alpha
培養ロット番号   033098 培養種別   distribution
培地   Dulbecco's modified Eagle's medium with 5% fetal calf serum (Mitsubishi, PVF01). 培養温度   37 C
継代時細胞数(濃度)   1-2 x 10^4 cells/sq.cm. 継代方法   Cells are harvested with 0.125% trypsin and 0.02% EDATA for 5 min. at room temperature.
増殖速度   NT 凍結時生細胞濃度   1.6x10^6
凍結直後生細胞率   96.0 使用抗生物質   free
継代数   P15* PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   
接着性   Yes 導入外部遺伝子   NT
凍結培地   Culture medium with 5% DMSO. 炭酸ガス濃度   
RFPL所見    追加情報   
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