JCRB0136.2 CHO(pMAM-HSluc)

細胞情報

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB0136.2 細胞名   CHO(pMAM-HSluc)
生物種(日本語)   チャイニーズハムスター 組織名(日本語)   卵巣
コメント(日本語)   CHO-K1由来, HSP 70Bプロモーターにルシフェラーゼレポーターを結合したpMAM-HSluc導入株 プロフィール   pMAM-HSluc transfected CHO-K1, HSP 70B gene promoter and luciferase reporter gene introduced
別名    動物名   hamster, Chinese
系統名   Chinese 学名・属名   Cricetulus
種名   griseus 性別   
年齢・月齢    細胞識別情報   
(癌)原発組織名   ovary 病歴情報   
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質   pMAM-HSluc transfected CHO-K1 cells. Luciferase activated at 42/43 C for several minutes. 細胞寿命   infinite
クライシスPDL    形態   
一般性状   HSP 70B gene promoter and luciferase gene included 細胞分類   transformed
細胞樹立者名   Mizuno,S. and et al. 細胞寄託者   Mizuno,S.
分譲時制限   not defined コメント   Refer CHO-K1 cell culture methods.
入手年   1998 培養培地   Ham's F12 medium with 10% fetal bovine serum.
継代方法   Cells are treated with 0.25% trypsin and 0.02% EDTA. 継代時細胞数   split ratio=1/10
人種    炭酸ガス濃度   5 %
採取組織名   genetically engineered CHO-K1 cells ウィルス検査   
検出ウィルス    組織型   
Reference
Pubmed id:9538196 Suppression of thermotolerance development through cycloheximide-induced negative control of stress protein gene expression.
Akagawa H,Ishii A,Mizuno S
J Biochem. 1998 Feb;123(2):226-32
Pubmed id:9113384 Stress dose-dependent suppression of heat shock protein gene expression by inhibiting protein synthesis during heat shock treatment.
Mizuno S,Ishii A,Murakami Y,Akagawa H
Cell Struct Funct. 1997 Feb;22(1):7-13
Pubmed id:8689565 Heat shock transcription factors: structure and regulation.
Wu C
Annu Rev Cell Dev Biol. 1995;11():441-69
Pubmed id:8355691 The DNA-binding activity of the human heat shock transcription factor is regulated in vivo by hsp70.
Mosser DD,Duchaine J,Massie B
Mol Cell Biol. 1993 Sep;13(9):5427-38
Pubmed id:3422754 Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals.
Rodriguez JF,Rodriguez D,Rodriguez JR,McGowan EB,Esteban M
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1667-71
Pubmed id:3821727 Firefly luciferase gene: structure and expression in mammalian cells.
de Wet JR,Wood KV,DeLuca M,Helinski DR,Subramani S
Mol Cell Biol. 1987 Feb;7(2):725-37
Pubmed id:17758108 Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants.
Ow DW,DE Wet JR,Helinski DR,Howell SH,Wood KV,Deluca M
Science. 1986 Nov 14;234(4778):856-9
Pubmed id:3927293 Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment.
Voellmy R,Ahmed A,Schiller P,Bromley P,Rungger D
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4949-53
Pubmed id:5271752 Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants.
Kao F,Chasin L,Puck TT
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1284-91

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LOT Information

細胞番号   JCRB0136.2 細胞名   
培養ロット番号   100499 培養種別   distribution
培地   Ham's F12 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) 培養温度   37 C
継代時細胞数(濃度)   1-2 x 10^5 cells/ml 継代方法   0.25% trypsin and 0.02% EDTA
増殖速度   D.T.=20-24 hrs 凍結時生細胞濃度   3.8 x 10^6
凍結直後生細胞率   95 使用抗生物質   free
継代数   P8* PDL数(プライマリ)   
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   Confirmed as Chinese hamster by NP, G6PD, MD.
染色体モード    染色体情報   
表面抗原    DNA Profile (STR)   
接着性    導入外部遺伝子   
凍結培地    炭酸ガス濃度   
RFPL所見    追加情報   
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