JCRB0136.1 CHO(pMAM-luc)

細胞情報

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB0136.1 細胞名   CHO(pMAM-luc)
生物種(日本語)   チャイニーズハムスター 組織名(日本語)   卵巣
コメント(日本語)   CHO-K1由来, ルシフェラーゼ遺伝子を含むpMAM-luc導入株 プロフィール   pMAM-luc transfected CHO-K1, luciferase activated by dexamethasone
別名    動物名   hamster, Chinese
系統名   Chinese 学名・属名   Cricetulus
種名   griseus 性別   
年齢・月齢    細胞識別情報   
(癌)原発組織名   ovary 病歴情報   genetically engineered CHO-K1 cells. External gene fragment(s) are introduced.
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質   Plasmid pMAM-luc transfected CHO-K1 cells. Luciferase activated by dexamethasone(2uM) at 37C, 5 hrs. 細胞寿命   infinite
クライシスPDL    形態   
一般性状   Luciferase gene under control of MMTV-LTR in plasmid. 細胞分類   transformed
細胞樹立者名   Mizuno,S. et al. 細胞寄託者   Mizuno,S.
分譲時制限   not identified コメント   See CHO-K1 cell culture methods.
入手年   1998 培養培地   Ham's F12 medium with 10% fetal bovine serum.
継代方法   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. 継代時細胞数   split ratio=1/10
人種    炭酸ガス濃度   5 %
採取組織名   ovary ウィルス検査   
検出ウィルス    組織型   
Reference
Pubmed id:9538196 Suppression of thermotolerance development through cycloheximide-induced negative control of stress protein gene expression.
Akagawa H,Ishii A,Mizuno S
J Biochem. 1998 Feb;123(2):226-32
Pubmed id:9113384 Stress dose-dependent suppression of heat shock protein gene expression by inhibiting protein synthesis during heat shock treatment.
Mizuno S,Ishii A,Murakami Y,Akagawa H
Cell Struct Funct. 1997 Feb;22(1):7-13
Pubmed id:8689565 Heat shock transcription factors: structure and regulation.
Wu C
Annu Rev Cell Dev Biol. 1995;11():441-69
Pubmed id:8355691 The DNA-binding activity of the human heat shock transcription factor is regulated in vivo by hsp70.
Mosser DD,Duchaine J,Massie B
Mol Cell Biol. 1993 Sep;13(9):5427-38
Pubmed id:3422754 Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals.
Rodriguez JF,Rodriguez D,Rodriguez JR,McGowan EB,Esteban M
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1667-71
Pubmed id:3821727 Firefly luciferase gene: structure and expression in mammalian cells.
de Wet JR,Wood KV,DeLuca M,Helinski DR,Subramani S
Mol Cell Biol. 1987 Feb;7(2):725-37
Pubmed id:17758108 Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants.
Ow DW,DE Wet JR,Helinski DR,Howell SH,Wood KV,Deluca M
Science. 1986 Nov 14;234(4778):856-9
Pubmed id:3927293 Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment.
Voellmy R,Ahmed A,Schiller P,Bromley P,Rungger D
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4949-53
Pubmed id:5271752 Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants.
Kao F,Chasin L,Puck TT
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1284-91

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LOT Information

細胞番号   JCRB0136.1 細胞名   
培養ロット番号   092999 培養種別   distribution
培地   Ham's F12 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) 培養温度   37 C
継代時細胞数(濃度)   1-2 x 10^5 cells/ml 継代方法   0.25% trypsin and 0.02% EDTA
増殖速度   D.T.=ca 24 hrs. 凍結時生細胞濃度   2.2 x 10^6
凍結直後生細胞率   98 使用抗生物質   free
継代数   P8* PDL数(プライマリ)   
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   Confirmed as Chinese hamster by NP, G6PD, MD.
染色体モード    染色体情報   
表面抗原    DNA Profile (STR)   
接着性    導入外部遺伝子   
凍結培地    炭酸ガス濃度   
RFPL所見    追加情報   
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