JCRB0140 NCE16
細胞情報
Important Notice(s)Supplements for the Serum free culture medium
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0140 | 細胞名 | NCE16 |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 子宮頸部 |
| コメント(日本語) | 不死化正常ケラチノサイト, HPV16による不死化 | プロフィール | Normal ecto-cervical keratinocyte cells immortalized by HPV16 virus genome. Growth of this cell line is dependent to EGF. |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | F |
| 年齢・月齢 | 細胞識別情報 | available | |
| （癌）原発組織名 | uterine cervix | 病歴情報 | normal keratinocyte immortalized with HPV16. |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | E6/E7 expressed. Telomerase expression observed. | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | keratinocyte | |
| 一般性状 | Normal ecto-cervical keratinocyte cells immortalized by HPV16. Growth is dependent to EGF. | 細胞分類 | immortalized |
| 細胞樹立者名 | Yasumoto,S. | 細胞寄託者 | Yasumoto,S. |
| 分譲時制限 | Not defined. | コメント | Any comments for subculture methods. |
| 入手年 | 1999 | 培養培地 | MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. MCDB153 with < 0.5% FCS was also used in original report. |
| 継代方法 | Cells are harvested after treatment with 0.1% trypsin and 0.01% EDTA. | 継代時細胞数 | Not defined. |
| 人種 | Japanese | 炭酸ガス濃度 | 5 % |
| 採取組織名 | uterine ectocervix | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:12821936 | Neurotrophin receptor p75(NTR) characterizes human esophageal keratinocyte stem cells in vitro. Okumura T,Shimada Y,Imamura M,Yasumoto S Oncogene. 2003 Jun 26;22(26):4017-26 |
| Pubmed id:9310137 | Two distinct human uterine cervical epithelial cell lines established after transfection with human papillomavirus 16 DNA. Ohta Y,Tsutsumi K,Kikuchi K,Yasumoto S Jpn J Cancer Res. 1997 Jul;88(7):644-51 |
| Pubmed id:9149908 | Time correlation of commitment to calcium-induced apoptosis and terminal differentiation in human ectocervical keratinocytes in suspension cultures. Kikuchi K,Tsutsumi K,Ohta Y,Yasumoto S Cell Growth Differ. 1997 May;8(5):571-9 |
| Pubmed id:8710384 | Telomerase activity in normal human epithelial cells. Yasumoto S,Kunimura C,Kikuchi K,Tahara H,Ohji H,Yamamoto H,Ide T,Utakoji T Oncogene. 1996 Jul 18;13(2):433-9 |
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LOT Information
| 細胞番号 | JCRB0140 | 細胞名 | |
|---|---|---|---|
| 培養ロット番号 | 07242006 | 培養種別 | distribution |
| 培地 | MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 10 ng EGF, and 50 ug/ml BPE | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 3 - 5 x 10^4 cells/ml | 継代方法 | Cells were harvested after treatment with 0.1% crystalline trypsin and 0.02% EDTA. Enzyme treatment was terminated by equal volume of 0.1% soybean trypsin inhibitor in PBS. Medium change every 2-3days |
| 増殖速度 | approx. 3-5 days | 凍結時生細胞濃度 | 6.0 x 10^5 |
| 凍結時生細胞率 | 75.1 | 使用抗生物質 | free |
| 継代数 | P27 | PDL数（プライマリ） | |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | Confirmed as human by NP, G6PD, AST. |
| 染色体モード | 染色体情報 | ||
| 表面抗原 | DNA Profile (STR) | D5S818:11,12 D13S317:11,13 D7S820:11,12 D16S539:11 VWA:16 TH01:7,9.3 AM:X TPOX:11 CSF1PO:11,13 |
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| 接着性 | Yes | 導入外部遺伝子 | |
| 凍結培地 | 10% DMSO, 0.1% methylcellulose - culture medium | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 追加情報 |
| 細胞番号 | JCRB0140 | 細胞名 | NCE16 |
|---|---|---|---|
| 培養ロット番号 | 113099 | 培養種別 | distribution |
| 培地 | MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 50 ug/ml bovine pituitary extract. | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 1-1.7 x 10^2 cells/sq.cm. | 継代方法 | Cells harvested with 0.1% trypsin and 0.01% EDTA. Change medium every 2-3 days and subculture every 6-7 days. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 1.4 x 10^6 |
| 凍結時生細胞率 | 93.3 | 使用抗生物質 | free |
| 継代数 | P26 | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | G6PD(typeB), NP and LDH examined. human non-HeLa. |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | D5S818:11,12 D13S317:11,13 D7S820:11,12 D16S539:11 VWA:16 TH01:7,10 AM:X TPOX:11 CSF1PO:11,13 |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | FM-1 and culture medium (1/1 mix) with 10% DMSO. | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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