JCRB0232.1 SKG-IIIb
細胞情報
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0232.1 | 細胞名 | SKG-IIIb |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 子宮頸部 |
| コメント(日本語) | 子宮頸部類上皮腫, 大細胞非角化型 | プロフィール | Human tumor cell line with non-keratinizing type squamous cell carcinoma from cervical tumor. Temperature sensitive growth was observed. |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | F |
| 年齢・月齢 | 38 | 細胞識別情報 | not done |
| （癌）原発組織名 | uterine cervix | 病歴情報 | large cell non-keratinizing type squamous cell carcinoma |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | tumor markers, TA-4, hCG, behta-hCG, PP7 | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | epithelial-like | |
| 一般性状 | The cell line was expected to be derived from an intermedially differentiated squamous cell carcinoma. Growth of the cell line SKG-IIIb was suppressed at 39 C. | 細胞分類 | tumor |
| 細胞樹立者名 | Udagawa,Y. & Nozawa,S. | 細胞寄託者 | Nozawa,S. |
| 分譲時制限 | コメント | The cell line was contaminated by mycoplasmas when deposited to the cell bank and they were eliminated in FDSC(JCRB). | |
| 入手年 | 1990 | 培養培地 | Ham's F12 (pH.7.2) medium with 10% fetal calf serum. |
| 継代方法 | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. | 継代時細胞数 | 1.5 x 10^5 cells/sq.cm. |
| 人種 | Japanese | 炭酸ガス濃度 | |
| 採取組織名 | cervix of uterus | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | Partial HPV16 DNA lacking E1 region is integrated in host genome, and apparently not exists as 8 kb circular DNA (revealed by Southern blot analysis (J. Gen. Virol, 68: 583-591, 1987)). | ||||||||
| Reference | |
|---|---|
| Pubmed id:11092985 | Regulation of dihydropyrimidine dehydrogenase and pyrimidine nucleoside phosphorylase activities by growth factors and subsequent effects on 5-fluorouracil sensitivity in tumor cells. Ueda M,Kitaura K,Kusada O,Mochizuki Y,Yamada N,Terai Y,Kumagai K,Ueki K,Ueki M Jpn J Cancer Res. 2000 Nov;91(11):1185-91 |
| Pubmed id:10545752 | Biological implications of growth factors on the mechanism of invasion in gynecological tumor cells. Ueda M,Ueki M,Terai Y,Ueki K,Kumagai K,Fujii H,Yoshizawa K,Nakajima M Gynecol Obstet Invest. 1999;48(3):221-8 |
| Pubmed id:9378537 | Stimulatory effects of EGF and TGF-alpha on invasive activity and 5'-deoxy-5-fluorouridine sensitivity in uterine cervical-carcinoma SKG-IIIb cells. Ueda M,Ueki M,Terai Y,Morimoto A,Fujii H,Yoshizawa K,Yanagihara T Int J Cancer. 1997 Sep 17;72(6):1027-33 |
| Pubmed id:9355022 | Inhibitory effects of ubenimex (bestatin) on the invasion of uterine cervical carcinoma cells and their production and activation of gelatinase A. Ueda M,Ueki M,Fujii H,Yoshizawa K,Nakajima M J Med. 1997;28(3-4):175-90 |
| Pubmed id:2212803 | [The mechanism of cytotoxicity of monoclonal antibodies to gynecological cancer cell-lines]. Iida S,Nozawa S Nihon Sanka Fujinka Gakkai Zasshi. 1990 Jul;42(7):667-74 |
| Pubmed id:3032185 | Conservation of E6 and E7 regions of human papillomavirus types 16 and 18 present in cervical cancers. Takebe N,Tsunokawa Y,Nozawa S,Terada M,Sugimura T Biochem Biophys Res Commun. 1987 Mar 30;143(3):837-44 |
| Pubmed id:3029293 | Integration and transcription of human papillomavirus type 16 and 18 sequences in cell lines derived from cervical carcinomas. Shirasawa H,Tomita Y,Sekiya S,Takamizawa H,Simizu B J Gen Virol. 1987 Feb;68 ( Pt 2)():583-91 |
| Pubmed id:3122483 | Anticellular activities of human fibroblast interferon beta and human recombinant interferon gamma. Cytologic changes in a cervical cancer cell line. Iwasaka T,Yoshimura T,Ohkuma Y,Tsugitomi H,Sugimori H Acta Cytol. 1987 Nov-Dec;31(6):941-5 |
| Pubmed id:3794454 | [Sensitivity to etoposide in cultured cells from cervical squamous cell carcinoma]. Maeda T,Ueda M,Yamada T,Kubo H,Okamura S,Sano T,Ueki M,Sugimoto O Nihon Sanka Fujinka Gakkai Zasshi. 1986 Nov;38(11):2050-6 |
| Pubmed id:6209350 | [Peplomycin sensitivity of various types of human gynecological cultured tumor cells]. Ueda M,Nozawa S,Ueki M,Sano T,Nagai S,Sugimoto O,Kurihara S Nihon Sanka Fujinka Gakkai Zasshi. 1984 Oct;36(10):1867-76 |
| Pubmed id:6699456 | [Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma]. Udagawa Y,Nozawa S,Tsukazaki K,Ohta H,Akiba T,Kurihara S Nihon Sanka Fujinka Gakkai Zasshi. 1984 Feb;36(2):237-46 |
| Pubmed id:6600963 | Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes. Nozawa S,Udagawa Y,Ohta H,Kurihara S,Fishman WH Cancer Res. 1983 Apr;43(4):1748-60 |
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LOT Information
| 細胞番号 | JCRB0232.1 | 細胞名 | SKG-IIIb |
|---|---|---|---|
| 培養ロット番号 | 08162001 | 培養種別 | distribution |
| 培地 | Ham's F12 medium with 10% fetal bovine serum (Intergen RB52405). | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 6.0 x 10^4 cells/ml | 継代方法 | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 5 minutes at 37 C. Subculture once a week and medium change twice a week. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 3.8 x 10^6 |
| 凍結時生細胞率 | 96.3 | 使用抗生物質 | free |
| 継代数 | P15* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | D5S818:10,11 D13S317:12 D7S820:10,12 D16S539:9,11 VWA:16 TH01:6,9 AM:X TPOX:8,11 CSF1PO:11,12 |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Culture medium containing 5% DMSO. | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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