細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB0232.1 細胞名   SKG-IIIb
生物種(日本語)   ヒト 組織名(日本語)   子宮頸部
コメント(日本語)   子宮頸部類上皮腫, 大細胞非角化型 プロフィール   Human tumor cell line with non-keratinizing type squamous cell carcinoma from cervical tumor. Temperature sensitive growth was observed.
別名    動物名   human
系統名    学名・属名   Homo
種名   sapiens 性別   F
年齢・月齢   38 細胞識別情報   not done
(癌)原発組織名   uterine cervix 病歴情報   large cell non-keratinizing type squamous cell carcinoma
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質   tumor markers, TA-4, hCG, behta-hCG, PP7 細胞寿命   infinite
クライシスPDL    形態   epithelial-like
一般性状   The cell line was expected to be derived from an intermedially differentiated squamous cell carcinoma. Growth of the cell line SKG-IIIb was suppressed at 39 C. 細胞分類   tumor
細胞樹立者名   Udagawa,Y. & Nozawa,S. 細胞寄託者   Nozawa,S.
分譲時制限    コメント   The cell line was contaminated by mycoplasmas when deposited to the cell bank and they were eliminated in FDSC(JCRB).
入手年   1990 培養培地   Ham's F12 (pH.7.2) medium with 10% fetal calf serum.
継代方法   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. 継代時細胞数   1.5 x 10^5 cells/sq.cm.
人種   Japanese 炭酸ガス濃度   
採取組織名   cervix of uterus 組織型   

ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR)
tested ウイルスRNA
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy)はneXtProtプロジェクトの一環として、SIB - Swiss Institute of BioinformaticsのCALIPHOグループのAmos Bairoch博士によって開発されました。Cellosaurusは細胞株に関する様々な情報を集約させたデータベースになっています。JCRB細胞バンクに登録されている細胞株については、右のリンクから情報を得ることができます。 CVCL_2795
Pubmed id:11092985Regulation of dihydropyrimidine dehydrogenase and pyrimidine nucleoside phosphorylase activities by growth factors and subsequent effects on 5-fluorouracil sensitivity in tumor cells.
Ueda M,Kitaura K,Kusada O,Mochizuki Y,Yamada N,Terai Y,Kumagai K,Ueki K,Ueki M
Jpn J Cancer Res. 2000 Nov;91(11):1185-91
Pubmed id:10545752Biological implications of growth factors on the mechanism of invasion in gynecological tumor cells.
Ueda M,Ueki M,Terai Y,Ueki K,Kumagai K,Fujii H,Yoshizawa K,Nakajima M
Gynecol Obstet Invest. 1999;48(3):221-8
Pubmed id:9378537Stimulatory effects of EGF and TGF-alpha on invasive activity and 5'-deoxy-5-fluorouridine sensitivity in uterine cervical-carcinoma SKG-IIIb cells.
Ueda M,Ueki M,Terai Y,Morimoto A,Fujii H,Yoshizawa K,Yanagihara T
Int J Cancer. 1997 Sep 17;72(6):1027-33
Pubmed id:9355022Inhibitory effects of ubenimex (bestatin) on the invasion of uterine cervical carcinoma cells and their production and activation of gelatinase A.
Ueda M,Ueki M,Fujii H,Yoshizawa K,Nakajima M
J Med. 1997;28(3-4):175-90
Pubmed id:2212803[The mechanism of cytotoxicity of monoclonal antibodies to gynecological cancer cell-lines].
Iida S,Nozawa S
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Jul;42(7):667-74
Pubmed id:3032185Conservation of E6 and E7 regions of human papillomavirus types 16 and 18 present in cervical cancers.
Takebe N,Tsunokawa Y,Nozawa S,Terada M,Sugimura T
Biochem Biophys Res Commun. 1987 Mar 30;143(3):837-44
Pubmed id:3029293Integration and transcription of human papillomavirus type 16 and 18 sequences in cell lines derived from cervical carcinomas.
Shirasawa H,Tomita Y,Sekiya S,Takamizawa H,Simizu B
J Gen Virol. 1987 Feb;68 ( Pt 2)():583-91
Pubmed id:3122483Anticellular activities of human fibroblast interferon beta and human recombinant interferon gamma. Cytologic changes in a cervical cancer cell line.
Iwasaka T,Yoshimura T,Ohkuma Y,Tsugitomi H,Sugimori H
Acta Cytol. 1987 Nov-Dec;31(6):941-5
Pubmed id:3794454[Sensitivity to etoposide in cultured cells from cervical squamous cell carcinoma].
Maeda T,Ueda M,Yamada T,Kubo H,Okamura S,Sano T,Ueki M,Sugimoto O
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Nov;38(11):2050-6
Pubmed id:6209350[Peplomycin sensitivity of various types of human gynecological cultured tumor cells].
Ueda M,Nozawa S,Ueki M,Sano T,Nagai S,Sugimoto O,Kurihara S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Oct;36(10):1867-76
Pubmed id:6699456[Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma].
Udagawa Y,Nozawa S,Tsukazaki K,Ohta H,Akiba T,Kurihara S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Feb;36(2):237-46
Pubmed id:6600963Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes.
Nozawa S,Udagawa Y,Ohta H,Kurihara S,Fishman WH
Cancer Res. 1983 Apr;43(4):1748-60


LOT Information

細胞番号   JCRB0232.1 細胞名   SKG-IIIb
培養ロット番号   08162001 培養種別   distribution
培地   Ham's F12 medium with 10% fetal bovine serum (Intergen RB52405). 培養温度   37 C
継代時細胞数(濃度)   6.0 x 10^4 cells/ml 継代方法   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 5 minutes at 37 C. Subculture once a week and medium change twice a week.
増殖速度   NT 凍結時生細胞濃度   3.8 x 10^6
凍結時生細胞率   96.3 使用抗生物質   free
継代数   P15* PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:10,11
接着性   Yes 導入外部遺伝子   NT
凍結培地   Culture medium containing 5% DMSO. 炭酸ガス濃度   5 %
RFPL所見   NT 解凍後生細胞率