JCRB0196 RP-3
細胞情報
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0196 | 細胞名 | RP-3 |
|---|---|---|---|
| 生物種(日本語) | マウス | 組織名(日本語) | 抗体産生ハイブリドーマ |
| コメント(日本語) | 抗ラット好中球IgM産生 | プロフィール | Mouse cell line producing IgM antibody against rat neutrophil. The antibody is harmfull to the rat neutrophil in vivo or in vitro with complement. |
| 別名 | 動物名 | mouse | |
| 系統名 | BALB/c | 学名・属名 | Mus |
| 種名 | musclus | 性別 | |
| 年齢・月齢 | 細胞識別情報 | not done | |
| （癌）原発組織名 | antibody-producing hybridoma | 病歴情報 | normal |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | Hybridoma X63 as parent. Rat neutrophil specific IgM production. | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | lymphocyte-like | |
| 一般性状 | Mouse cell line producing IgM antibody against the rat neutrophil. The antibody is harmfull to the rat neutrophil in vivo or in vitro with complement. | 細胞分類 | hybridoma |
| 細胞樹立者名 | Gotoh,S. and Sendo,F. et. al. | 細胞寄託者 | Sendo,F. |
| 分譲時制限 | コメント | ||
| 入手年 | 2000 | 培養培地 | RPMI1640 medium with 10% fetal calf serum. Use Iscov's modified Dulbecco's medium with 10% fetal calf serum when cell dose not grow well. |
| 継代方法 | Simple dilution. | 継代時細胞数 | not definde. |
| 人種 | 炭酸ガス濃度 | 5 % | |
| 採取組織名 | ascites | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
not tested | ウイルスRNA 検出検査 |
not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9748561 | Effect of neutrophil depletion in acute cerebritis. Lo WD,Chen R,Boue DR,Stokes BT Brain Res. 1998 Aug 17;802(1-2):175-83 |
| Pubmed id:9524329 | Selective neutrophil depletion with monoclonal antibodies attenuates ischemia/reperfusion injury in skeletal muscle. Iwahori Y,Ishiguro N,Shimizu T,Kondo S,Yabe Y,Oshima T,Iwata H,Sendo F J Reconstr Microsurg. 1998 Feb;14(2):109-16 |
| Pubmed id:9504889 | Selective depletion of neutrophils by a monoclonal antibody, RP-3, suppresses dextran sulphate sodium-induced colitis in rats. Natsui M,Kawasaki K,Takizawa H,Hayashi SI,Matsuda Y,Sugimura K,Seki K,Narisawa R,Sendo F,Asakura H J Gastroenterol Hepatol. 1997 Dec;12(12):801-8 |
| Pubmed id:8686765 | Role of neutrophils in the pathogenesis of experimental vasculitis. Qasim FJ,Mathieson PW,Sendo F,Thiru S,Oliveira DB Am J Pathol. 1996 Jul;149(1):81-9 |
| Pubmed id:8999285 | Reduced anti-Pseudomonas aeruginosa activity of a cephalosporin, cefodizime, in rats whose neutrophils were selectively depleted by a monoclonal antibody. Araki A,Sendo F Microbiol Immunol. 1996;40(5):333-8 |
| Pubmed id:8027557 | Modulation of the in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. III. Enhancement by RP-3 treatment of the anti-sheep red blood cell plaque-forming cell response in rats. Tamura M,Sekiya S,Terashita M,Sendo F J Immunol. 1994 Aug 1;153(3):1301-8 |
| Pubmed id:8473729 | Modulation of in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. I. Inhibition by RP-3 treatment of the priming and effector phases of delayed type hypersensitivity to sheep red blood cells in rats. Kudo C,Yamashita T,Araki A,Terashita M,Watanabe T,Atsumi M,Tamura M,Sendo F J Immunol. 1993 May 1;150(9):3728-38 |
| Pubmed id:8473730 | Modulation of in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. II. Inhibition by RP-3 treatment of mononuclear leukocyte recruitment in delayed-type hypersensitivity to sheep red blood cells in rats. Kudo C,Yamashita T,Terashita M,Sendo F J Immunol. 1993 May 1;150(9):3739-46 |
| Pubmed id:8478140 | Abrogation of tumor-inhibitory MRC-OX8+ (CD8+) effector T-cell generation in rats by selective depletion of neutrophils in vivo using a monoclonal antibody. Tanaka E,Sendo F Int J Cancer. 1993 Apr 22;54(1):131-6 |
| Pubmed id:8445327 | Inhibition of Corynebacterium parvum-primed and lipopolysaccharide-induced hepatic necrosis in rats by selective depletion of neutrophils using a monoclonal antibody. Sato T,Shinzawa H,Abe Y,Takahashi T,Arai S,Sendo F J Leukoc Biol. 1993 Feb;53(2):144-50 |
| Pubmed id:1338643 | Correlation between reduction of polymorphonuclear leucocytes in glomeruli injected with a newly developed monoclonal antineutrophil antibody and proteinuria in Masugi nephritis. Yaguchi Y,Tomino Y,Ozaki T,Okumura K,Sendo F,Koide H Nephron. 1992;62(4):444-8 |
| Pubmed id:1881586 | Neutrophil as a mediator of ischemic edema formation in the brain. Shiga Y,Onodera H,Kogure K,Yamasaki Y,Yashima Y,Syozuhara H,Sendo F Neurosci Lett. 1991 Apr 29;125(2):110-2 |
| Pubmed id:2120340 | Vascular hyperpermeability induced by tumor necrosis factor and its augmentation by IL-1 and IFN-gamma is inhibited by selective depletion of neutrophils with a monoclonal antibody. Abe Y,Sekiya S,Yamasita T,Sendo F J Immunol. 1990 Nov 1;145(9):2902-7 |
| Pubmed id:2119251 | Modulation of the immune response to transplanted tumors in rats by selective depletion of neutrophils in vivo using a monoclonal antibody: abrogation of specific transplantation resistance to chemical carcinogen-induced syngeneic tumors by selective depletion of neutrophils in vivo. Midorikawa Y,Yamashita T,Sendo F Cancer Res. 1990 Oct 1;50(19):6243-7 |
| Pubmed id:2391449 | Suppression of late phase enhanced vascular permeability in rats by selective depletion of neutrophils with a monoclonal antibody. Sekiya S,Yamashita T,Sendo F J Leukoc Biol. 1990 Sep;48(3):258-65 |
| Pubmed id:2746139 | Selective depletion of rat neutrophils by in vivo administration of a monoclonal antibody. Sekiya S,Gotoh S,Yamashita T,Watanabe T,Saitoh S,Sendo F J Leukoc Biol. 1989 Aug;46(2):96-102 |
| Pubmed id:2424975 | Enhancement of the expression of a rat neutrophil-specific cell surface antigen by activation with phorbol myristate acetate and concanavalin A. Gotoh S,Itoh M,Fujii Y,Arai S,Sendo F J Immunol. 1986 Jul 15;137(2):643-50 |
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LOT Information
| 細胞番号 | JCRB0196 | 細胞名 | RP-3 |
|---|---|---|---|
| 培養ロット番号 | 12182001 | 培養種別 | distribution |
| 培地 | RPMI1640 medium with 10% heat-inactivated(56C,30min.) fetal bovine serum (Intergen RB52405). | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 1.7 x 10^5 cells/ml | 継代方法 | Simple dilution and subculture every 3 days. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 1.2 x 10^6 |
| 凍結時生細胞率 | 80.5 | 使用抗生物質 | free |
| 継代数 | P2* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | No | 導入外部遺伝子 | NT |
| 凍結培地 | Culture medium with 5% DMSO. | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |