JCRB0196 RP-3

細胞情報

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB0196 細胞名   RP-3
生物種(日本語)   マウス 組織名(日本語)   抗体産生ハイブリドーマ
コメント(日本語)   抗ラット好中球IgM産生 プロフィール   Mouse cell line producing IgM antibody against rat neutrophil. The antibody is harmfull to the rat neutrophil in vivo or in vitro with complement.
別名    動物名   mouse
系統名   BALB/c 学名・属名   Mus
種名   musclus 性別   
年齢・月齢    細胞識別情報   not done
(癌)原発組織名   antibody-producing hybridoma 病歴情報   normal
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質   Hybridoma X63 as parent. Rat neutrophil specific IgM production. 細胞寿命   infinite
クライシスPDL    形態   lymphocyte-like
一般性状   Mouse cell line producing IgM antibody against the rat neutrophil. The antibody is harmfull to the rat neutrophil in vivo or in vitro with complement. 細胞分類   hybridoma
細胞樹立者名   Gotoh,S. and Sendo,F. et. al. 細胞寄託者   Sendo,F.
分譲時制限    コメント   
入手年   2000 培養培地   RPMI1640 medium with 10% fetal calf serum. Use Iscov's modified Dulbecco's medium with 10% fetal calf serum when cell dose not grow well.
継代方法   Simple dilution. 継代時細胞数   not definde.
人種    炭酸ガス濃度   5 %
採取組織名   ascites ウィルス検査   
検出ウィルス    組織型   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy)はneXtProtプロジェクトの一環として、SIB - Swiss Institute of BioinformaticsのCALIPHOグループのAmos Bairoch博士によって開発されました。Cellosaurusは細胞株に関する様々な情報を集約させたデータベースになっています。JCRB細胞バンクに登録されている細胞株については、右のリンクから情報を得ることができます。 CVCL_G343
Reference
Pubmed id:9524329 Selective neutrophil depletion with monoclonal antibodies attenuates ischemia/reperfusion injury in skeletal muscle.
Iwahori Y,Ishiguro N,Shimizu T,Kondo S,Yabe Y,Oshima T,Iwata H,Sendo F
J Reconstr Microsurg. 1998 Feb;14(2):109-16
Pubmed id:9748561 Effect of neutrophil depletion in acute cerebritis.
Lo WD,Chen R,Boue DR,Stokes BT
Brain Res. 1998 Aug 17;802(1-2):175-83
Pubmed id:9504889 Selective depletion of neutrophils by a monoclonal antibody, RP-3, suppresses dextran sulphate sodium-induced colitis in rats.
Natsui M,Kawasaki K,Takizawa H,Hayashi SI,Matsuda Y,Sugimura K,Seki K,Narisawa R,Sendo F,Asakura H
J Gastroenterol Hepatol. 1997 Dec;12(12):801-8
Pubmed id:8686765 Role of neutrophils in the pathogenesis of experimental vasculitis.
Qasim FJ,Mathieson PW,Sendo F,Thiru S,Oliveira DB
Am J Pathol. 1996 Jul;149(1):81-9
Pubmed id:8999285 Reduced anti-Pseudomonas aeruginosa activity of a cephalosporin, cefodizime, in rats whose neutrophils were selectively depleted by a monoclonal antibody.
Araki A,Sendo F
Microbiol Immunol. 1996;40(5):333-8
Pubmed id:8027557 Modulation of the in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. III. Enhancement by RP-3 treatment of the anti-sheep red blood cell plaque-forming cell response in rats.
Tamura M,Sekiya S,Terashita M,Sendo F
J Immunol. 1994 Aug 1;153(3):1301-8
Pubmed id:8445327 Inhibition of Corynebacterium parvum-primed and lipopolysaccharide-induced hepatic necrosis in rats by selective depletion of neutrophils using a monoclonal antibody.
Sato T,Shinzawa H,Abe Y,Takahashi T,Arai S,Sendo F
J Leukoc Biol. 1993 Feb;53(2):144-50
Pubmed id:8473729 Modulation of in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. I. Inhibition by RP-3 treatment of the priming and effector phases of delayed type hypersensitivity to sheep red blood cells in rats.
Kudo C,Yamashita T,Araki A,Terashita M,Watanabe T,Atsumi M,Tamura M,Sendo F
J Immunol. 1993 May 1;150(9):3728-38
Pubmed id:8478140 Abrogation of tumor-inhibitory MRC-OX8+ (CD8+) effector T-cell generation in rats by selective depletion of neutrophils in vivo using a monoclonal antibody.
Tanaka E,Sendo F
Int J Cancer. 1993 Apr 22;54(1):131-6
Pubmed id:8473730 Modulation of in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. II. Inhibition by RP-3 treatment of mononuclear leukocyte recruitment in delayed-type hypersensitivity to sheep red blood cells in rats.
Kudo C,Yamashita T,Terashita M,Sendo F
J Immunol. 1993 May 1;150(9):3739-46
Pubmed id:1338643 Correlation between reduction of polymorphonuclear leucocytes in glomeruli injected with a newly developed monoclonal antineutrophil antibody and proteinuria in Masugi nephritis.
Yaguchi Y,Tomino Y,Ozaki T,Okumura K,Sendo F,Koide H
Nephron. 1992;62(4):444-8
Pubmed id:1881586 Neutrophil as a mediator of ischemic edema formation in the brain.
Shiga Y,Onodera H,Kogure K,Yamasaki Y,Yashima Y,Syozuhara H,Sendo F
Neurosci Lett. 1991 Apr 29;125(2):110-2
Pubmed id:2120340 Vascular hyperpermeability induced by tumor necrosis factor and its augmentation by IL-1 and IFN-gamma is inhibited by selective depletion of neutrophils with a monoclonal antibody.
Abe Y,Sekiya S,Yamasita T,Sendo F
J Immunol. 1990 Nov 1;145(9):2902-7
Pubmed id:2119251 Modulation of the immune response to transplanted tumors in rats by selective depletion of neutrophils in vivo using a monoclonal antibody: abrogation of specific transplantation resistance to chemical carcinogen-induced syngeneic tumors by selective depletion of neutrophils in vivo.
Midorikawa Y,Yamashita T,Sendo F
Cancer Res. 1990 Oct 1;50(19):6243-7
Pubmed id:2391449 Suppression of late phase enhanced vascular permeability in rats by selective depletion of neutrophils with a monoclonal antibody.
Sekiya S,Yamashita T,Sendo F
J Leukoc Biol. 1990 Sep;48(3):258-65
Pubmed id:2746139 Selective depletion of rat neutrophils by in vivo administration of a monoclonal antibody.
Sekiya S,Gotoh S,Yamashita T,Watanabe T,Saitoh S,Sendo F
J Leukoc Biol. 1989 Aug;46(2):96-102
Pubmed id:2424975 Enhancement of the expression of a rat neutrophil-specific cell surface antigen by activation with phorbol myristate acetate and concanavalin A.
Gotoh S,Itoh M,Fujii Y,Arai S,Sendo F
J Immunol. 1986 Jul 15;137(2):643-50

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LOT Information

細胞番号   JCRB0196 細胞名   RP-3
培養ロット番号   12182001 培養種別   distribution
培地   RPMI1640 medium with 10% heat-inactivated(56C,30min.) fetal bovine serum (Intergen RB52405). 培養温度   37 C
継代時細胞数(濃度)   1.7 x 10^5 cells/ml 継代方法   Simple dilution and subculture every 3 days.
増殖速度   NT 凍結時生細胞濃度   1.2 x 10^6
凍結時生細胞率   80.5 使用抗生物質   free
継代数   P2* PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   
接着性   No 導入外部遺伝子   NT
凍結培地   Culture medium with 5% DMSO. 炭酸ガス濃度   5 %
RFPL所見   NT 解凍後生細胞率   
追加情報   
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