JCRB1033 NOZ

細胞情報

Important Notice(s)

On the agreement for distribution of JHH series cell lines, NOZ and OZ

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB1033 細胞名   NOZ
生物種(日本語)   ヒト 組織名(日本語)   肝臓, 胆嚢
コメント(日本語)   胆嚢がん, 中分化型管状腺がん プロフィール   Human gallbladder carcinoma cell line.
別名    動物名   human
系統名    学名・属名   Homo
種名   sapiens 性別   F
年齢・月齢   48 細胞識別情報   available
(癌)原発組織名   liver, gallbladder 病歴情報   peritonitis carcinomatosa. Gallbladder carcinoma of adenocarcinoma tubular, moderately differentiated. AFP 420ng/ml, CEA 1.3 ng/ml.
転移の有無(Y/N)   Yes (癌)転移組織名   ascites
遺伝的性質   No. of chromosome = 46. 細胞寿命   infinite
クライシスPDL    形態   epithelial-like
一般性状   Dt=48 hrs. Plating efficiency is 14-19%. Cells are transplantable to nude mice and the morphology of the transplanted tumor was similar to the original one. 細胞分類   tumor
細胞樹立者名   Honma,S. & Nagamori,S. 細胞寄託者   Nagamori,S.
分譲時制限   Require negotiation and agreement from Dr.Nagamori before shipping. コメント   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly.
入手年   2001 培養培地   Williams'E medium with 10% fetal calf serum.
継代方法   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. 継代時細胞数   
人種   Japanese 炭酸ガス濃度   5 %
採取組織名   ascites ウィルス検査   tested
検出ウィルス   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested,GAPDH is positive control] 組織型   moderately differentiated adenocarcinoma.
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy)はneXtProtプロジェクトの一環として、SIB - Swiss Institute of BioinformaticsのCALIPHOグループのAmos Bairoch博士によって開発されました。Cellosaurusは細胞株に関する様々な情報を集約させたデータベースになっています。JCRB細胞バンクに登録されている細胞株については、右のリンクから情報を得ることができます。 CVCL_3079
Reference
Pubmed id:2543327 [Combination therapy of hyperthermia and other methods in liver and bile tract cancers--evaluation of these methods using cancer cell lines in vitro].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1905-12


Research results by users. Click!
Pubmed id:24655726 Oridonin induces apoptosis and cell cycle arrest of gallbladder cancer cells via the mitochondrial pathway.
Bao R,Shu Y,Wu X,Weng H,Ding Q,Cao Y,Li M,Mu J,Wu W,Ding Q,Tan Z,Liu T,Jiang L,Hu Y,Gu J,Liu Y
BMC Cancer. 2014 Mar 21;14():217
Pubmed id:25165862 Schisandrin B induces apoptosis and cell cycle arrest of gallbladder cancer cells.
Xiang SS,Wang XA,Li HF,Shu YJ,Bao RF,Zhang F,Cao Y,Ye YY,Weng H,Wu WG,Mu JS,Wu XS,Li ML,Hu YP,Jiang L,Tan ZJ,Lu W,Liu F,Liu YB
Molecules. 2014 Aug 27;19(9):13235-50
Pubmed id:24966896 miR-1 and miR-145 act as tumor suppressor microRNAs in gallbladder cancer.
Letelier P,García P,Leal P,Álvarez H,Ili C,López J,Castillo J,Brebi P,Roa JC
Int J Clin Exp Pathol. 2014;7(5):1849-67
Pubmed id:25010679 Forkhead box L1 is frequently downregulated in gallbladder cancer and inhibits cell growth through apoptosis induction by mitochondrial dysfunction.
Qin Y,Gong W,Zhang M,Wang J,Tang Z,Quan Z
PLoS One. 2014;9(7):e102084
Pubmed id:25090123 Cordycepin induces S phase arrest and apoptosis in human gallbladder cancer cells.
Wang XA,Xiang SS,Li HF,Wu XS,Li ML,Shu YJ,Zhang F,Cao Y,Ye YY,Bao RF,Weng H,Wu WG,Mu JS,Hu YP,Jiang L,Tan ZJ,Lu W,Wang P,Liu YB
Molecules. 2014 Jul 31;19(8):11350-65
Pubmed id:25277242 Expression of the RIP-1 gene and its role in growth and invasion of human gallbladder carcinoma.
Zhu G,Chen X,Wang X,Li X,Du Q,Hong H,Tang N,She F,Chen Y
Cell Physiol Biochem. 2014;34(4):1152-65
Pubmed id:24568162 Baicalin induces apoptosis of gallbladder carcinoma cells in vitro via a mitochondrial-mediated pathway and suppresses tumor growth in vivo.
Shu YJ,Bao RF,Wu XS,Weng H,Ding Q,Cao Y,Li ML,Mu JS,Wu WG,Ding QC,Liu TY,Jiang L,Hu YP,Tan ZJ,Wang P,Liu YB
Anticancer Agents Med Chem. 2014;14(8):1136-45
Pubmed id:25154789 Tumor necrosis factor-α promotes the lymphangiogenesis of gallbladder carcinoma through nuclear factor-κB-mediated upregulation of vascular endothelial growth factor-C.
Du Q,Jiang L,Wang X,Wang M,She F,Chen Y
Cancer Sci. 2014 Oct;105(10):1261-71
Pubmed id:24329682 Inhibitory effects of deleted in liver cancer 1 gene on gallbladder cancer growth through induction of cell cycle arrest and apoptosis.
Qin Y,Chu B,Gong W,Wang J,Tang Z,Shen J,Quan Z
J Gastroenterol Hepatol. 2014 May;29(5):964-72
Pubmed id:24756644 UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest.
Qin Y,Wang J,Gong W,Zhang M,Tang Z,Zhang J,Quan Z
Oncol Rep. 2014 Jun;31(6):2635-43
Pubmed id:24217644 Role of aquaporin-5 in gallbladder carcinoma.
Sekine S,Shimada Y,Nagata T,Sawada S,Yoshioka I,Matsui K,Moriyama M,Omura T,Osawa S,Shibuya K,Hashimoto I,Watanabe T,Hojo S,Hori R,Okumura T,Yoshida T,Tsukada K
Eur Surg Res. 2013;51(3-4):108-17
Pubmed id:23830367 Combination chemotherapy of nafamostat mesylate with gemcitabine for gallbladder cancer targeting nuclear factor-κB activation.
Iwase R,Haruki K,Fujiwara Y,Furukawa K,Shiba H,Uwagawa T,Misawa T,Ohashi T,Yanaga K
J Surg Res. 2013 Sep;184(1):605-12
Pubmed id:22835954 Synergic effect of photodynamic therapy using talaporfin sodium with conventional anticancer chemotherapy for the treatment of bile duct carcinoma.
Nonaka Y,Nanashima A,Nonaka T,Uehara M,Isomoto H,Abo T,Nagayasu T
J Surg Res. 2013 May;181(2):234-41
Pubmed id:22071224 Vascular endothelial growth factor-D promotes growth, lymphangiogenesis and lymphatic metastasis in gallbladder cancer.
Lin W,Jiang L,Chen Y,She F,Han S,Zhu J,Zhou L,Tang N,Wang X,Li X
Cancer Lett. 2012 Jan 28;314(2):127-36
Pubmed id:22201822 SNCG gene silencing in gallbladder cancer cells inhibits key tumorigenic activities.
Han S,She F,Wang D,Yao X,Jiang L,Chen Y
Front Biosci (Landmark Ed). 2012 Jan 1;17():1589-98
Pubmed id:22843894 Establishment and characterization of a new human gallbladder carcinoma cell line.
Sekine S,Shimada Y,Nagata T,Moriyama M,Omura T,Yoshioka I,Hori R,Matsui K,Sawada S,Okumura T,Yoshida T,Tsukada K
Anticancer Res. 2012 Aug;32(8):3211-8
Pubmed id:22320835 Adenovirus-mediated gene transfer of tissue factor pathway inhibitor-2 inhibits gallbladder carcinoma growth in vitro and in vivo.
Qin Y,Zhang S,Gong W,Li J,Jia J,Quan Z
Cancer Sci. 2012 Apr;103(4):723-30
Pubmed id:21360081 Advantages of laserphyrin compared with photofrin in photodynamic therapy for bile duct carcinoma.
Nonaka T,Nanashima A,Nonaka M,Uehara M,Isomoto H,Nonaka Y,Nagayasu T
J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):592-600
Pubmed id:20464561 Novel photodynamic therapy against biliary tract carcinoma using mono-L: -aspartyl chlorine e6: basic evaluation for its feasibility and efficacy.
Kasuya K,Shimazu M,Suzuki M,Kuroiwa Y,Usuda J,Itoi T,Tsuchida A,Aoki T
J Hepatobiliary Pancreat Sci. 2010 May;17(3):313-21
Pubmed id:20651359 Analysis of apoptotic effects induced by photodynamic therapy in a human biliary cancer cell line.
Nonaka T,Nanashima A,Nonaka M,Uehara M,Isomoto H,Asahina I,Nagayasu T
Anticancer Res. 2010 Jun;30(6):2113-8
Pubmed id:20734221 Vascular endothelial growth factor-C promotes the growth and invasion of gallbladder cancer via an autocrine mechanism.
Chen Y,Jiang L,She F,Tang N,Wang X,Li X,Han S,Zhu J
Mol Cell Biochem. 2010 Dec;345(1-2):77-89
Pubmed id:19209029 Schedule-dependent synergistic interaction between gemcitabine and oxaliplatin in human gallbladder adenocarcinoma cell lines.
Makiyama A,Qin B,Uchino K,Shibata Y,Arita S,Isobe T,Hirano G,Kusaba H,Baba E,Akashi K,Nakano S
Anticancer Drugs. 2009 Feb;20(2):123-30
Pubmed id:18204862 Upregulation of topoisomerase IIalpha expression in advanced gallbladder carcinoma: a potential chemotherapeutic target.
Washiro M,Ohtsuka M,Kimura F,Shimizu H,Yoshidome H,Sugimoto T,Seki N,Miyazaki M
J Cancer Res Clin Oncol. 2008 Jul;134(7):793-801
Pubmed id:17235714 Antitumor effect of gemcitabine on orthotopically inoculated human gallbladder cancer cells in nude mice.
Mita Y,Ajiki T,Kamigaki T,Okazaki T,Hori H,Horiuchi H,Hirata K,Fujita T,Fujimori T,Kuroda Y
Ann Surg Oncol. 2007 Apr;14(4):1374-80
Pubmed id:15855925 Oncolytic adenoviral therapy in gallbladder carcinoma.
Tekant Y,Davydova J,Ramirez PJ,Curiel DT,Vickers SM,Yamamoto M
Surgery. 2005 May;137(5):527-35
Pubmed id:15743030 A MEK inhibitor (U0126) markedly inhibits direct liver invasion of orthotopically inoculated human gallbladder cancer cells in nude mice.
Horiuchi H,Kawamata H,Furihata T,Omotehara F,Hori H,Shinagawa Y,Ohkura Y,Tachibana M,Yamazaki T,Ajiki T,Kuroda Y,Fujimori T
J Exp Clin Cancer Res. 2004 Dec;23(4):599-606
Pubmed id:12963974 A MEK inhibitor (U0126) prolongs survival in nude mice bearing human gallbladder cancer cells with K-ras mutation: analysis in a novel orthotopic inoculation model.
Horiuchi H,Kawamata H,Fujimori T,Kuroda Y
Int J Oncol. 2003 Oct;23(4):957-63
Pubmed id:2543327 [Combination therapy of hyperthermia and other methods in liver and bile tract cancers--evaluation of these methods using cancer cell lines in vitro].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1905-12
Pubmed id:3154020 [Establishment and characterization of a human gall bladder carcinoma cell line NOZ].
Homma S,Hasumura S,Nagamori S,Kameda H
Hum Cell. 1988 Mar;1(1):95-7
Pubmed id:none バイオテクノロジー素材としてのヒト癌細胞 (1)。胆嚢癌NOZ。蛋白質。核酸
永森静志
蛋白質・核酸・酵素 33, 2605-2606 (1988)
Pubmed id:none Morphological changes in human gall bladder carcinoma cell line-NOZ due to epirubicin and doxorubicin
Masaaki Kawada, Hideki Aizaki, Kenichi Fukaya, Minoru Niiya, Tomokazu Matsuura, Hajime Sujino, Satoshi Hasumura, Seishi Nagamori, Gotaro Toda
Medical Electron Microscopy 27, 175-177 (1994)
Pubmed id:none 閉塞性黄疸に対するPTCD胆汁細胞診とK-ras点突然変異の解析
味木 徹夫, 藤盛 孝博, 塚本 龍子, 渥美 亜紀子, 小野山 裕彦, 前田 盛, 斎藤 洋一
日本臨床細胞学会雑誌 34(3), 424-428 (1995)
Pubmed id:none 微小管を標的とした抗癌剤のヒト胆嚢癌細胞に対する効果に関する基礎的研究
柴本 由香, 高木 一郎
胆道 14(1), 42-50 (2000)

Images



LOT Information

細胞番号   JCRB1033 細胞名   NOZ
培養ロット番号   08052009 培養種別   distribution
培地   William's E medium with10% heat inactivated fetal bovine serum(Intergen RB52305). 培養温度   37 C
継代時細胞数(濃度)   2.8x10^4 cells/sq.cm 継代方法   Cells were harvested after treatment with 0.25%(GIBCO) trypsin and 0.02% EDTA.
増殖速度   NT 凍結時生細胞濃度   4.5x10^6
凍結時生細胞率   99.1 使用抗生物質   free
継代数   p7* PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:11,13
D13S317:8,12
D7S820:10,11
D16S539:9,11
VWA:19
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:11,13
接着性   Yes 導入外部遺伝子   NT
凍結培地   Cell Banker BLC-1(Nihon Zenyaku Industries) 炭酸ガス濃度   5 %
RFPL所見    解凍後生細胞率   
追加情報   
Images
細胞番号   JCRB1033 細胞名   
培養ロット番号   09162008 培養種別   distribution
培地   William's medium E with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) 培養温度   37 C
継代時細胞数(濃度)   1 - 2 x 10^5 cells/mL 継代方法   0.25% trypsin and 0.02% EDTA
増殖速度   30 hrs 凍結時生細胞濃度   4.8 x 10^6
凍結時生細胞率   97.5 使用抗生物質   free
継代数   Unknown (8 at bank) PDL数(プライマリ)   
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   Confirmed as human by NP, G6PD (type B), MD.
染色体モード    染色体情報   
表面抗原    DNA Profile (STR)   
接着性   Yes 導入外部遺伝子   
凍結培地   10% DMSO, 20% FBS - William's medium E 炭酸ガス濃度   5%
RFPL所見    解凍後生細胞率   
追加情報   
細胞番号   JCRB1033 細胞名   NOZ
培養ロット番号   02122003 培養種別   distribution
培地   William's E medium with 10% fetal calf serum(Intergen RB52305). 培養温度   37 C
継代時細胞数(濃度)   2.0 x 10^4 cells/sq.cm. 継代方法   Cells were harvested after treatment with 0.2% trypsin and 0.02% EDTA. Medium change twice a week and subculture once a week.
増殖速度   NT 凍結時生細胞濃度   4.5 x 10^6
凍結時生細胞率   98.7 使用抗生物質   free
継代数   P5* PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   G6PD(typeB), NP, LDH examined. Human, not HeLa.
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:11,13
D13S317:8,12
D7S820:10,11
D16S539:9,11
VWA:19
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:11,13
接着性   Yes 導入外部遺伝子   NT
凍結培地   Culture medium containing 5% DMSO. 炭酸ガス濃度   5 %
RFPL所見   NT 解凍後生細胞率   
追加情報   
Images
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