JCRB1040 FF101
細胞情報
細胞種類:一般細胞 (細胞分譲手数料はこちら)
細胞番号(JCRB) | JCRB1040 | 細胞名 | FF101 |
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生物種(日本語) | ラット | 組織名(日本語) | 肝臓 |
コメント(日本語) | 肝細胞がん, 3'-methyl-4-dimethylaminoazobenzene誘発, 無血清培養, (Donryu rat) | プロフィール | Rat hepatoma cell line with producing unidentified growth factor maintained in serum-free RPMI1640 medium for 444 passages. |
別名 | 動物名 | rat | |
系統名 | donryu | 学名・属名 | Rattus |
種名 | norvegicus | 性別 | |
年齢・月齢 | adult | 細胞識別情報 | |
(癌)原発組織名 | liver | 病歴情報 | hepatoma was induced by feeding 0.06% 3'-methyl-4-dimethylaminoazobenzene for five months. |
転移の有無(Y/N) | (癌)転移組織名 | ||
遺伝的性質 | Cells have characteristics of hepatoma and cholangioma. | 細胞寿命 | infinite |
クライシスPDL | 形態 | epithelial-like | |
一般性状 | The cells are expected to produce FF-GF (unidentified growth factor, 70KDa). Sup. of the cell culture have growth activating activity(AH66, K562, Y3, YAC-1,BALB/c,3T3,NS-1). Cells have been cultured with serum-free medium from the primary cuture. | 細胞分類 | tumor |
細胞樹立者名 | Matsumoto,H.et.al. | 細胞寄託者 | Matsumoto,H |
分譲時制限 | not defined | コメント | Cells have been maintained by continuous cell culture without freezing at all. |
入手年 | 2002 | 培養培地 | Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). |
継代方法 | Adhesion to dishes a weak. Pipetting with 2-3 times is enough to harvest cells. Subculture once every 7-10 days. | 継代時細胞数 | not defined. |
人種 | 炭酸ガス濃度 | 5 % | |
採取組織名 | liver | 組織型 |
ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
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ウイルスDNA 検出検査 |
not tested | ウイルスRNA 検出検査 |
not tested |
Reference | |
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Pubmed id:1281996 | Purification and characterization of a novel growth factor (FF-GF) synthesized by a rat hepatoma cell line, FF101. Matsuda H,Matsumoto M,Kitahara H,Haraguchi S,Kanai K Biochem Biophys Res Commun. 1992 Dec 15;189(2):654-61 |
Pubmed id:2702652 | Partial purification of a growth factor synthesized by a rat hepatoma cell line established in serum-free medium. Matsuda H,Matsumoto M,Haraguchi S,Kanai K Cancer Res. 1989 Apr 15;49(8):2118-22 |
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LOT Information
細胞番号 | JCRB1040 | 細胞名 | FF101 |
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培養ロット番号 | 10282003 | 培養種別 | distribution |
培地 | Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). | 培養温度 | 37C |
継代時細胞数(濃度) | 3.4 x 10^4 cells/sq.cm | 継代方法 | Pipetting with 2-3 times is enough to harvest cells. |
増殖速度 | NT | 凍結時生細胞濃度 | 1.7 x 10^6 |
凍結時生細胞率 | 86.2 | 使用抗生物質 | Free |
継代数 | P455 | PDL数(プライマリ) | NT |
マイコプラズマ検出 | - | 細菌汚染検出 | - |
真菌汚染検出 | - | アイソザイム検査・動物名 | NT |
染色体モード | NT | 染色体情報 | NT |
表面抗原 | NT | DNA Profile (STR) | |
接着性 | Yes | 導入外部遺伝子 | NT |
凍結培地 | Culture medium + FM-1 (1 to 1 mix) | 炭酸ガス濃度 | 5% |
解凍後生細胞率 | 追加情報 |
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