JCRB1047 OVSAYO

細胞情報

Important Notice(s)

Problem of JCRB1047 OVSAYO、JCRB1049 OVMIU、JCRB1050 OVMIU-II

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB1047 細胞名   OVSAYO
生物種(日本語)   ヒト 組織名(日本語)   卵巣
コメント(日本語)   腺がん, (OVMIUと同一のDNAプロファイルを示し、クロスコンタミの疑いあり) プロフィール   Human cell line derived from ovarian tumour.
別名    動物名   human
系統名    学名・属名   Homo
種名   sapiens 性別   F
年齢・月齢   64 細胞識別情報   available
(癌)原発組織名   ovary 病歴情報   ovarian carcinoma, stage Ic, not treated before.
転移の有無(Y/N)   No (癌)転移組織名   
遺伝的性質   ER(-), PR(-) / tumour markers, CA125, SLX, TPA(x). 細胞寿命   infinite
クライシスPDL    形態   epithelial-like
一般性状   Cells were not transplantable to mouse. 細胞分類   tumour
細胞樹立者名   Gorai,I. et.al. 細胞寄託者   Gorai,I.
分譲時制限   User information should be reported to Dr. Gorai promptly. コメント   The STR pattern of the OVSAYO was consistent with the OVMIU while it was expected to be unique.
入手年   2002 培養培地   RPMI1640 medium with 10% fetal bovine serum.
継代方法   Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days. 継代時細胞数   5 x 10^4 cells/ml
人種   Japanese 炭酸ガス濃度   5 %
採取組織名   ovary ウィルス検査   tested [-/negative, +/positive, nt/not tested,GAPDH is positive control]
検出ウィルス   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-) 組織型   adeno carcinoma
Reference
Pubmed id:11121861 Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells.
Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S
Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139 Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20.
Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H
Br J Cancer. 1997;76(7):829-35
Pubmed id:7882618 Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice.
Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M
Clin Exp Metastasis. 1995 Mar;13(2):89-96
Pubmed id:7535723 Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties.
Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H
Gynecol Oncol. 1995 Apr;57(1):33-46

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LOT Information

細胞番号   JCRB1047 細胞名   OVSAYO
培養ロット番号   03242003 培養種別   distribution
培地   RPMI1640 medium with 10% fetal calf serum (Intergen RB52305). 培養温度   37 C
継代時細胞数(濃度)   1.5 x 10^4 cells/sq.cm. 継代方法   Cells harvested after treatment with 0.05% trypsin for less than 15 minutes.
増殖速度   NT 凍結時生細胞濃度   2.0 x 10^6
凍結直後生細胞率   97.5 使用抗生物質   free
継代数   P39 PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   G6PD(type B), NP, LDH examined. Human not HeLa.
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:11,12
D13S317:8,11
D7S820:10,12
D16S539:9,10
VWA:17,18
TH01:7
AM:X
TPOX:9,11
CSF1PO:12
接着性   Yes 導入外部遺伝子   NT
凍結培地   Culture medium containing 5% DMSO. 炭酸ガス濃度   5 %
RFPL所見   Unique 追加情報   
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