JCRB1070 HSGc-C5
細胞情報
Important Notice(s)HSGc-C5 cell line: the problem of origin (HeLa-derivative)
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB1070 | 細胞名 | HSGc-C5 |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | HeLa汚染株 |
| コメント(日本語) | HeLa汚染株, 樹立時は唾液腺由来細胞株として報告されていた。重粒子線研究に利用される | プロフィール | Human undifferentiated carcinoma cell line from floor of mouth (HeLa evidences observed). |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | M |
| 年齢・月齢 | 54 | 細胞識別情報 | available |
| （癌）原発組織名 | HeLa-contaminant | 病歴情報 | diagnosed as a keratinizing squamous cell carcinoma and the patient was treated with a total of 3000 rads of external cobalt-60 irradiation resulted a decrease in size. |
| 転移の有無（Y/N） | No | （癌）転移組織名 | no |
| 遺伝的性質 | 細胞寿命 | infinite | |
| ｸﾗｲｼｽＰＤＬ | 形態 | epitherial-like(?) | |
| 一般性状 | Raddiation sensitivity D0=2.7Gy, D10=4.1Gy, alpha=0.31Gy^-1, beta=0.062Gy^-2 (200kVp X ray). Td=23hr. Plating efficiency=75-85%. | 細胞分類 | tumor |
| 細胞樹立者名 | Shirasuna,K. | 細胞寄託者 | Furusawa,Y. |
| 分譲時制限 | User of this cell line must cite the referece {5443}. | コメント | Although the STR-PCR analysis showed this cell line is genetically same cell line as the HeLa, but this cell line will be kept in the catalogue list of the JCRB cell bank. |
| 入手年 | 2003 | 培養培地 | Eagle's minimal essential medium (SIGMA, M4655) with 10% fetal bovine serum. |
| 継代方法 | Cells are rinsed twice with PBS(-), rinsed with 0.02% trypsin and kept at 37C for 3 minutes. | 継代時細胞数 | 2-5 x 10^5 cells/5ml in T25. |
| 人種 | Japanese | 炭酸ガス濃度 | 5 % |
| 採取組織名 | floor of the mouth | 組織型 | Undifferentiated adenocarcinoma |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | + | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:12377347 | Preclinical biological assessment of proton and carbon ion beams at Hyogo Ion Beam Medical Center. Kagawa K,Murakami M,Hishikawa Y,Abe M,Akagi T,Yanou T,Kagiya G,Furusawa Y,Ando K,Nojima K,Aoki M,Kanai T Int J Radiat Oncol Biol Phys. 2002 Nov 1;54(3):928-38 |
| Pubmed id:11025645 | Inactivation of aerobic and hypoxic cells from three different cell lines by accelerated (3)He-, (12)C- and (20)Ne-ion beams. Furusawa Y,Fukutsu K,Aoki M,Itsukaichi H,Eguchi-Kasai K,Ohara H,Yatagai F,Kanai T,Ando K Radiat Res. 2000 Nov;154(5):485-96 |
| Pubmed id:3359442 | Growth inhibition and differentiation of human salivary adenocarcinoma cells by medium conditioned with normal human fibroblasts. Shirasuna K,Morioka S,Watatani K,Hayashido Y,Furusawa H,Sugiyama M,Okura M,Matsuya T Cancer Res. 1988 May 15;48(10):2819-24 |
| Pubmed id:2417703 | Isolation and characterization of different clones including myoepithelial-like variants from a clonal neoplastic epithelial duct cell line of human salivary gland origin. Shirasuna K,Watatani K,Sugiyama M,Morioka S,Miyazaki T Cancer Res. 1986 Mar;46(3):1418-26 |
| Pubmed id:7248901 | A neoplastic epithelial duct cell line established from an irradiated human salivary gland. Shirasuna K,Sato M,Miyazaki T Cancer. 1981 Aug 1;48(3):745-52 |
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LOT Information
| 細胞番号 | JCRB1070 | 細胞名 | HSGc-C5 |
|---|---|---|---|
| 培養ロット番号 | 02132004 | 培養種別 | distribution |
| 培地 | Eagle's minimal essential medium with 10% fetal bovine serum (Intergen RB52305). | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 5.4 x 10^3 cells/sq.cm. | 継代方法 | Cells harvested after treatment with 0.2% trypsin at 37 C for three minutes. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 1.7 x 10^6 |
| 凍結時生細胞率 | 99.4 | 使用抗生物質 | Free |
| 継代数 | P8* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Culture medium containing 5% DMSO. | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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| 細胞番号 | JCRB1070 | 細胞名 | HSGc-C5 |
|---|---|---|---|
| 培養ロット番号 | 10072003 | 培養種別 | distribution |
| 培地 | Eagle's minimal essential medium with 10% fetal calf serum(Intergen RB52305). | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 0.5-6x10^4 cells/sq.cm. | 継代方法 | Cells harvested after treatment with 0.02% trypsin. Subculture every 4-7 days. Medium change every 2-3 days. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 2.6 x 10^6 |
| 凍結時生細胞率 | 97.5 | 使用抗生物質 | free |
| 継代数 | P4* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | G6PD(typeA), NP, LDH examined. HeLa. See CellID. |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Culture medium with 5% DMSO. | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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