JCRB0607.1 MK.P3
細胞情報
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0607.1 | 細胞名 | MK.P3 |
|---|---|---|---|
| 生物種(日本語) | カニクイザル | 組織名(日本語) | 腎臓 |
| コメント(日本語) | MK (JTC-12)細胞由来, 無血清培養 | プロフィール | Serum free cell culture of the MK(monkey kidney, JTC-12) cell. |
| 別名 | JTC-12.P3 | 動物名 | monkey, Cynomolgus |
| 系統名 | Cynomologus | 学名・属名 | Macaca |
| 種名 | fascicularis | 性別 | F |
| 年齢・月齢 | 細胞識別情報 | ||
| （癌）原発組織名 | kidney | 病歴情報 | normal |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | kidney | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | epitherial-like | |
| 一般性状 | Adapted to serum free DM-160 medium and maintained by Takaoka,T. for long passages by continuous cultuivation without freezing. | 細胞分類 | deposit |
| 細胞樹立者名 | Katsuta,H. | 細胞寄託者 | Takaoka,T. |
| 分譲時制限 | コメント | Usage of serum cause changes of the cell characetristics. Serum free medium is essential to keep the characteristics of this cell line. JCRB0607 will be eliminated and the NIHS0354 will be opened for the distribution. | |
| 入手年 | 2004 | 培養培地 | DM160 medium. No serum required. |
| 継代方法 | Harvest cells with rubber policeman. | 継代時細胞数 | |
| 人種 | 炭酸ガス濃度 | 5 % | |
| 採取組織名 | kidney | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
not tested | ウイルスRNA 検出検査 |
not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7905235 | Propagation of hepatitis A virus in a renal cell line JTC-12.P3 of cynomolgus monkey origin. Baba M,Takegawa M,Kaito M,Miyamoto K,Suzuki S Acta Virol. 1993 Aug;37(4):209-22 |
| Pubmed id:3142784 | A new approach to the modification of cell membrane glycosphingolipids: ganglioside composition of JTC-12 P3 cells altered by feeding with galactose as a sole carbohydrate source in protein- and lipid-free synthetic medium. Kawaguchi T,Takaoka T,Yoshida E,Iwamori M,Takatsuki K,Nagai Y Exp Cell Res. 1988 Dec;179(2):507-16 |
| Pubmed id:3530606 | Regulation of sodium-coupled phosphate transport by extracellular phosphate in cultured kidney cells (JTC-12). Takuwa Y,Takeuchi Y,Ogata E Clin Sci (Lond). 1986 Sep;71(3):307-12 |
| Pubmed id:3933482 | Characterization of Na+-dependent phosphate uptake in cultured kidney cells (JTC-12) from monkey. Takuwa Y,Ogata E Biochem J. 1985 Sep 15;230(3):715-21 |
| Pubmed id:2863248 | Differentiated properties characteristic of renal proximal epithelium in a cell line derived from a normal monkey kidney (JTC-12). Takuwa Y,Ogata E In Vitro Cell Dev Biol. 1985 Aug;21(8):445-9 |
| Pubmed id:208643 | Hormone-specific responses and biosynthesis of sulfolipids in cell lines derived from mammalian kidney. Ishizuka I,Tadano K,Nagata N,Niimura Y,Nagai Y Biochim Biophys Acta. 1978 Jul 17;541(4):467-82 |
| Pubmed id:351092 | [Topics on sulfated glycolipids (author's transl)]. Ishizuka I Seikagaku. 1978 Mar 25;50(3):145-59 |
| Pubmed id:13984589 | Establishment of a cell strain, JTC-12, from cynomolgus monkey kidney tissue. TAKAOKA T,KATSUTA H,ENDO M,SATO K,OKUMURA H Jpn J Exp Med. 1962 Aug;32():351-68 |
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LOT Information
| 細胞番号 | JCRB0607.1 | 細胞名 | MK.P3 |
|---|---|---|---|
| 培養ロット番号 | 10052004 | 培養種別 | distribution |
| 培地 | DM201 (serum-free medium) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 1.5 x 10^4 / sq.cm | 継代方法 | Cells harvested with scraper. Subculture 10-14 days, medium change every 3-4 days. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 2.38x10^6 |
| 凍結時生細胞率 | 73.3 | 使用抗生物質 | NT |
| 継代数 | P10* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | One to one mix with Cultue medium and FM-1(containing 10%DMSO) medium. | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 追加情報 |
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