JCRB1083 KYSE180

細胞情報

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB1083 細胞名   KYSE180
生物種(日本語)   ヒト 組織名(日本語)   食道
コメント(日本語)   扁平上皮がん, 高分化型, (民間企業利用者は、分譲依頼前に細胞樹立者とMTAを手交する必要があります。) プロフィール   Human well differentiated squamous cell carcinoma from esophageal cancer.
別名    動物名   human
系統名    学名・属名   Homo
種名   sapiens 性別   M
年齢・月齢   53 細胞識別情報   available
(癌)原発組織名   esophagus 病歴情報   esophagus cancer, T4N1Mo Stage 3, squamous cell carcinoma (SCC)
転移の有無(Y/N)   No (癌)転移組織名   
遺伝的性質   cyclin D1 amplification, p53 mutation 細胞寿命   infinite
クライシスPDL    形態   epithelial-like
一般性状   Xenotransplantation positive. 細胞分類   tumor
細胞樹立者名   Shimada,Y. 細胞寄託者   Shimada,Y.
分譲時制限   Cite reference {5368}. Company users require MTA from the originator. See additional information. コメント   Distribution restricted to Japan(domestic use only).
入手年   2003 培養培地   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum.
継代方法   Cells harvested after treatment with trypsin and EDTA. 継代時細胞数   split ratio = 1/5
人種   Japanese 炭酸ガス濃度   5 %
採取組織名   esophagus ウィルス検査   tested
検出ウィルス   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested, GAPDH = positive control] 組織型   Well differenciated squamous cell carcinoma.
Reference
Pubmed id:11092977 Nonrandom chromosomal imbalances in esophageal squamous cell carcinoma cell lines: possible involvement of the ATF3 and CENPF genes in the 1q32 amplicon.
Pimkhaokham A,Shimada Y,Fukuda Y,Kurihara N,Imoto I,Yang ZQ,Imamura M,Nakamura Y,Amagasa T,Inazawa J
Jpn J Cancer Res. 2000 Nov;91(11):1126-33
Pubmed id:10446988 Lymph node metastasis is associated with allelic loss on chromosome 13q12-13 in esophageal squamous cell carcinoma.
Harada H,Tanaka H,Shimada Y,Shinoda M,Imamura M,Ishizaki K
Cancer Res. 1999 Aug 1;59(15):3724-9
Pubmed id:9699676 Methylation of the 5' CpG island of the FHIT gene is closely associated with transcriptional inactivation in esophageal squamous cell carcinomas.
Tanaka H,Shimada Y,Harada H,Shinoda M,Hatooka S,Imamura M,Ishizaki K
Cancer Res. 1998 Aug 1;58(15):3429-34
Pubmed id:9033652 Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860 Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:9816044 p53 mutation, murine double minute 2 amplification, and human papillomavirus infection are frequently involved but not associated with each other in esophageal squamous cell carcinoma.
Shibagaki I,Tanaka H,Shimada Y,Wagata T,Ikenaga M,Imamura M,Ishizaki K
Clin Cancer Res. 1995 Jul;1(7):769-73
Pubmed id:7913084 Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M
Int J Cancer. 1994 Jul 15;58(2):291-7
Pubmed id:8518899 Prognostic significance of cell culture in carcinoma of the oesophagus.
Shimada Y,Imamura M
Br J Surg. 1993 May;80(5):605-7
Pubmed id:1728357 Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84

Images



LOT Information

細胞番号   JCRB1083 細胞名   KYSE180
培養ロット番号   07252008 培養種別   distribution
培地   RPMI1640(GIBCO) and Ham's F12 medium(GIBCO)(1/1) with 2% heat inactivated fetal bovine serum(Hyclone GRD0051) 培養温度   37 C
継代時細胞数(濃度)   1.9x10^4 cells/sq.cm 継代方法   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
増殖速度   NT 凍結時生細胞濃度   2.2x10^6
凍結直後生細胞率   98.9 使用抗生物質   free (this lot was pretreated with plasmocin)
継代数   p715 PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:8,11
D13S317:9,12
D7S820:9,12
D16S539:9,10
VWA:16,17
TH01:7
AM:X
TPOX:11
CSF1PO:12,13
接着性   Yes 導入外部遺伝子   NT
凍結培地   Cell Banker BLC-1(Nihon Zenyaku Industries) 炭酸ガス濃度   5 %
RFPL所見   NT 追加情報   
Images
細胞番号   JCRB1083 細胞名   KYSE180
培養ロット番号   08012008 培養種別   distribution
培地   RPMI1640(GIBCO) and Ham's F12 medium(GIBCO)(1/1) with 2% heat inactivated fetal bovine serum(Hyclone GRD0051) 培養温度   37 C
継代時細胞数(濃度)   1.9x10^4 cells/sq.cm 継代方法   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
増殖速度   NT 凍結時生細胞濃度   4.0x10^6
凍結直後生細胞率   99.0 使用抗生物質   free (this lot was pretreated with BM cyclin 1and
継代数   p716 PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:8,11
D13S317:9,12
D7S820:9,12
D16S539:9,10
VWA:16,17
TH01:7
AM:X
TPOX:11
CSF1PO:12,13
接着性   Yes 導入外部遺伝子   NT
凍結培地   Cell Banker BLC-1(Nihon Zenyaku Industries) 炭酸ガス濃度   5 %
RFPL所見   NT 追加情報   
Images
細胞番号   JCRB1083 細胞名   KYSE180
培養ロット番号   10222004 培養種別   distribution
培地   1 to 1 mix of Ham's F12 and RPMI1640 medium containing 2% fetal calf serum. 培養温度   37 C
継代時細胞数(濃度)   4.0 x 10^4 cells / sq. cm 継代方法   Cells were harvested after treatment with 0.25% trypsin about 5 min. Subculture once a week and medium change every 2-4 days.
増殖速度   NT 凍結時生細胞濃度   3.8x10^6
凍結直後生細胞率   96 使用抗生物質   free
継代数   P715 PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   
接着性   Yes 導入外部遺伝子   NT
凍結培地   Culture medium containing 5% DMSO at final concentration. 炭酸ガス濃度   5%
RFPL所見   NT 追加情報   
Images
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