JCRB1086 KYSE220
細胞情報
Important Notice(s)On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)
Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB1086 | 細胞名 | KYSE220 |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 食道 |
| コメント(日本語) | 扁平上皮がん, 中分化型, (民間企業利用者は、分譲依頼前に細胞樹立者とMTAを手交する必要があります。) | プロフィール | Human squamous cell carcinoma from esophagus. |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | M |
| 年齢・月齢 | 78 | 細胞識別情報 | available |
| （癌）原発組織名 | esophagus | 病歴情報 | esophagus cancer |
| 転移の有無（Y/N） | No | （癌）転移組織名 | |
| 遺伝的性質 | amplification of int-2 and hst-1 observed, Species were verified by HLA analysis | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | epithelial-like | |
| 一般性状 | T3N1M0 Stage 3. Both p53 and p16 mutations were observed. No virus infections were observed. | 細胞分類 | tumor |
| 細胞樹立者名 | Shimada,Y. | 細胞寄託者 | Shimada,Y. |
| 分譲時制限 | Cite reference {5368}. Company users require MTA from the originator. See additional information. | コメント | Distribution restricted to Japan(domestic use only). |
| 入手年 | 2004 | 培養培地 | 1 to 1 mix of Ham's F12 and RPMI1640 medium containing 2% fetal calf serum. |
| 継代方法 | 継代時細胞数 | split ratio = 1/5 | |
| 人種 | Japanese | 炭酸ガス濃度 | 5 % |
| 採取組織名 | esophagus | 組織型 | Moderately differenciated SCC |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:11092977 | Nonrandom chromosomal imbalances in esophageal squamous cell carcinoma cell lines: possible involvement of the ATF3 and CENPF genes in the 1q32 amplicon. Pimkhaokham A,Shimada Y,Fukuda Y,Kurihara N,Imoto I,Yang ZQ,Imamura M,Nakamura Y,Amagasa T,Inazawa J Jpn J Cancer Res. 2000 Nov;91(11):1126-33 |
| Pubmed id:10446988 | Lymph node metastasis is associated with allelic loss on chromosome 13q12-13 in esophageal squamous cell carcinoma. Harada H,Tanaka H,Shimada Y,Shinoda M,Imamura M,Ishizaki K Cancer Res. 1999 Aug 1;59(15):3724-9 |
| Pubmed id:9699676 | Methylation of the 5' CpG island of the FHIT gene is closely associated with transcriptional inactivation in esophageal squamous cell carcinomas. Tanaka H,Shimada Y,Harada H,Shinoda M,Hatooka S,Imamura M,Ishizaki K Cancer Res. 1998 Aug 1;58(15):3429-34 |
| Pubmed id:9033652 | Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines. Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K Int J Cancer. 1997 Feb 7;70(4):437-42 |
| Pubmed id:8575860 | Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K Int J Cancer. 1996 Jan 26;65(3):372-6 |
| Pubmed id:9816044 | p53 mutation, murine double minute 2 amplification, and human papillomavirus infection are frequently involved but not associated with each other in esophageal squamous cell carcinoma. Shibagaki I,Tanaka H,Shimada Y,Wagata T,Ikenaga M,Imamura M,Ishizaki K Clin Cancer Res. 1995 Jul;1(7):769-73 |
| Pubmed id:7913084 | Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M Int J Cancer. 1994 Jul 15;58(2):291-7 |
| Pubmed id:8518899 | Prognostic significance of cell culture in carcinoma of the oesophagus. Shimada Y,Imamura M Br J Surg. 1993 May;80(5):605-7 |
| Pubmed id:1728357 | Characterization of 21 newly established esophageal cancer cell lines. Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T Cancer. 1992 Jan 15;69(2):277-84 |
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LOT Information
| 細胞番号 | JCRB1086 | 細胞名 | |
|---|---|---|---|
| 培養ロット番号 | 04152008 | 培養種別 | distribution |
| 培地 | 1:1 mixture of Ham's F12 medium and RPMI 1640 medium with 2% fetal bovine serum (FBS Lot; GIBCO Cat. No. 10099, Lot 484375) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 0.6 - 1.5 x 10^5 cells/ml | 継代方法 | 0.25% trypsin and 0.02% EDTA at 37 C for 5 min. |
| 増殖速度 | approx. 16 hrs | 凍結時生細胞濃度 | 6.3 x 10^6 |
| 凍結時生細胞率 | 97.2 | 使用抗生物質 | free |
| 継代数 | P287 | PDL数（プライマリ） | |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | Confirmed as human by NP, G6PD (type B), MD. |
| 染色体モード | 染色体情報 | ||
| 表面抗原 | DNA Profile (STR) | ||
| 接着性 | Yes | 導入外部遺伝子 | |
| 凍結培地 | 10% DMSO, 20% FBS - culture medium | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 追加情報 |
| 細胞番号 | JCRB1086 | 細胞名 | KYSE220 |
|---|---|---|---|
| 培養ロット番号 | 10122004 | 培養種別 | distribution |
| 培地 | Mixed medium(1/1) of the RPMI1640 and the F12 medium containing 2% fetal calf serum (Intergen RB52305). | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 2.7 x 10^4 cells/sq.cm. | 継代方法 | Cells were harvested after treatment with 0.25% trypsin. Subculture once a week and medium change every 2-3 days. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 2.1 x 10^6 |
| 凍結時生細胞率 | 95.6 | 使用抗生物質 | free |
| 継代数 | P285 | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Culture medium containing 5 % DMSO. | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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