JCRB1137 UE6E7TC-4
細胞情報
Important Notice(s)細胞種類:一般細胞 (細胞分譲手数料はこちら)
細胞番号(JCRB) | JCRB1137 | 細胞名 | UE6E7TC-4 |
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生物種(日本語) | ヒト | 組織名(日本語) | 間葉系幹細胞 |
コメント(日本語) | 軟骨由来不死化脱分化軟骨細胞株, 間葉系幹細胞マーカー発現, hTERT, HPV E6, E7導入 | プロフィール | Human immortalized mesenchymal cells derived from dedifferentiated chondrocyte, expressing mesenchymal stem cell markers. |
別名 | UEETC-4, TdHC1 | 動物名 | human |
系統名 | 学名・属名 | Homo | |
種名 | sapiens | 性別 | |
年齢・月齢 | 細胞識別情報 | available | |
(癌)原発組織名 | 病歴情報 | ||
転移の有無(Y/N) | No | (癌)転移組織名 | |
遺伝的性質 | hTERT, HPV E6, HPV E7 gene introducedstem cells which were infected with recombinant retroviruses expressing the E6, E7, and hTERT. | 細胞寿命 | infinite |
クライシスPDL | 形態 | fibroblast-like | |
一般性状 | Parent line: DEC1, dedifferentiated choncrytes (ref. Exp Cell Res, 288: 35-50, 2003). This cell line was immortalized with introduction of hTERT, HPV E6 E7 genes to DEC1 cells. positive for CD44, CD73, CD90, CD105, negative for CD34, CD45 (FACS analysis | 細胞分類 | normal |
細胞樹立者名 | Umezawa, A. | 細胞寄託者 | Umezawa, A. |
分譲時制限 | コメント | ||
入手年 | 2005 | 培養培地 | Poweredby10 |
継代方法 | When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells. | 継代時細胞数 | |
人種 | Japanese | 炭酸ガス濃度 | 5% |
採取組織名 | cartilage (DEC1 cells) | 組織型 |
ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
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ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
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Pubmed id:18545679 | Gremlin enhances the determined path to cardiomyogenesis. Kami D,Shiojima I,Makino H,Matsumoto K,Takahashi Y,Ishii R,Naito AT,Toyoda M,Saito H,Watanabe M,Komuro I,Umezawa A PLoS One. 2008 Jun 11;3(6):e2407 |
Pubmed id:15923633 | Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential. Mori T,Kiyono T,Imabayashi H,Takeda Y,Tsuchiya K,Miyoshi S,Makino H,Matsumoto K,Saito H,Ogawa S,Sakamoto M,Hata J,Umezawa A Mol Cell Biol. 2005 Jun;25(12):5183-95 |
Pubmed id:15293342 | Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation? Takeda Y,Mori T,Imabayashi H,Kiyono T,Gojo S,Miyoshi S,Hida N,Ita M,Segawa K,Ogawa S,Sakamoto M,Nakamura S,Umezawa A J Gene Med. 2004 Aug;6(8):833-45 |
Pubmed id:12878157 | Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis. Imabayashi H,Mori T,Gojo S,Kiyono T,Sugiyama T,Irie R,Isogai T,Hata J,Toyama Y,Umezawa A Exp Cell Res. 2003 Aug 1;288(1):35-50 |
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LOT Information
細胞番号 | JCRB1137 | 細胞名 | UE6E7TC-4 |
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培養ロット番号 | 05142021 | 培養種別 | distribution |
培地 | POWEREDBY10(GlycoTechnica Ltd.,catalog No.6012 ) | 培養温度 | 37 C |
継代時細胞数(濃度) | 0.7-1.2x10^4 cells/sq.cm | 継代方法 | Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.(1/3-1/4 split) |
増殖速度 | NT | 凍結時生細胞濃度 | 3.1x10^6 |
凍結時生細胞率 | 99.10 | 使用抗生物質 | free |
継代数 | p11* | PDL数(プライマリ) | NT |
マイコプラズマ検出 | - | 細菌汚染検出 | - |
真菌汚染検出 | - | アイソザイム検査・動物名 | NT |
染色体モード | NT | 染色体情報 | NT |
表面抗原 | NT | DNA Profile (STR) | D5S818:11,12 D13S317:14 D7S820:10,13 D16S539:9,11 VWA:15,16 TH01:7,9 AM:X TPOX:8,11 CSF1PO:10,11 |
接着性 | Yes | 導入外部遺伝子 | NT |
凍結培地 | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | 炭酸ガス濃度 | 5% |
解凍後生細胞率 | 95.30 | 追加情報 |
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