JCRB1152 RSMG-2
細胞情報
Important Notice(s)Use and cultivation of RSMG-1 and RSMG-2 cell lines
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB1152 | 細胞名 | RSMG-2 |
|---|---|---|---|
| 生物種(日本語) | ラット | 組織名(日本語) | 唾液腺 |
| コメント(日本語) | 顎下腺由来上皮細胞株, (Wistar rat) | プロフィール | Stem cell like cell line from male rat submandibular gland. |
| 別名 | 動物名 | rat | |
| 系統名 | Wistar | 学名・属名 | Rattus |
| 種名 | norvegicus | 性別 | M |
| 年齢・月齢 | 10-week-old | 細胞識別情報 | |
| （癌）原発組織名 | 病歴情報 | normal | |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | 細胞寿命 | infinite | |
| ｸﾗｲｼｽＰＤＬ | 形態 | epithelial-like | |
| 一般性状 | Growth of the RSMG-2 cells are inhibited by activin A, while accelarated by follistatin. The RSMG-2 cells shows a stem cell like characteristics. | 細胞分類 | normal |
| 細胞樹立者名 | Furue,M. | 細胞寄託者 | Furue,M. |
| 分譲時制限 | Must cite the references, {5541,5544}. | コメント | Co-operative research with Dr. Furue,M. recommended because of the special characteristics of the RSMG cells. |
| 入手年 | 2004 | 培養培地 | Serum free MCDB153 medium (Research Inst. for Functional Peptides, Yamagata, Japan). |
| 継代方法 | Cells are harvested after treatment with 0.05% trypsin in 0.04% EDTA then inactivated by 0.1% soybean trypain inhibitor. | 継代時細胞数 | split ratio = 1/4 |
| 人種 | 炭酸ガス濃度 | 5 % | |
| 採取組織名 | selection from whole body culture | 組織型 | submandibular gland epithelial cells |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
not tested | ウイルスRNA 検出検査 |
tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
|
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:11401526 | Activin A induces expression of rat Sel-1l mRNA, a negative regulator of notch signaling, in rat salivary gland-derived epithelial cells. Furue M,Zhang Y,Okamoto T,Hata RI,Asashima M Biochem Biophys Res Commun. 2001 Apr 6;282(3):745-9 |
| Pubmed id:10937830 | Isoleucine prevents rat salivary gland epithelial cells from apoptosis in serum-free culture. Furue M,Okamoto T,Koshika S,Asashima M In Vitro Cell Dev Biol Anim. 2000 May;36(5):287-9 |
| Pubmed id:10476908 | Effects of hepatocyte growth factor (HGF) and activin A on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture. Furue M,Okamoto T,Hayashi H,Sato JD,Asashima M,Saito S In Vitro Cell Dev Biol Anim. 1999 Mar;35(3):131-5 |
| Pubmed id:9719409 | Hepatocyte growth factor regulates activin betaA mRNA in submandibular gland. Furue M,Saito S In Vitro Cell Dev Biol Anim. 1998 Jul-Aug;34(7):520-3 |
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LOT Information
| 細胞番号 | JCRB1152 | 細胞名 | RSMG-2 |
|---|---|---|---|
| 培養ロット番号 | 08072008 | 培養種別 | distribution |
| 培地 | MCDB153+5F+F | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 1.0x10^4 cells/sq.cm | 継代方法 | Cells were harvested after treatment with 0.05% trypsin(GIBCO) and 0.04% EDTA |
| 増殖速度 | NT | 凍結時生細胞濃度 | 1.3x10^6 |
| 凍結時生細胞率 | 89.7 | 使用抗生物質 | NT |
| 継代数 | p26 | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | MCDB153+5F+F+FAF-BSA with 10% DMSO | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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