JCRB1426 KYSE190
細胞情報
Important Notice(s)Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB1426 | 細胞名 | KYSE190 |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 食道 |
| コメント(日本語) | 扁平上皮がん, 高分化型, (民間企業利用者は、分譲依頼前に細胞樹立者とMTAを手交する必要があります。) | プロフィール | Human well differentiated squamous cell carcinoma cell line established from esophageal cancer. |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | F |
| 年齢・月齢 | 69 | 細胞識別情報 | available |
| （癌）原発組織名 | esophagus | 病歴情報 | well differentiated squamous cell carcinoma, stage 2b |
| 転移の有無（Y/N） | Yes | （癌）転移組織名 | |
| 遺伝的性質 | 細胞寿命 | infinite | |
| ｸﾗｲｼｽＰＤＬ | 形態 | epithelial-like | |
| 一般性状 | T2N1M0 stage 2b | 細胞分類 | tumor |
| 細胞樹立者名 | Shimada, Y. | 細胞寄託者 | Shimada, Y. |
| 分譲時制限 | コメント | ||
| 入手年 | 2011 | 培養培地 | Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum (original description). Current medium for propagation; RPMI1640/F12 (1:1 mix) + 5 % FBS. |
| 継代方法 | Cells harvested after treatment with trypsin and EDTA. | 継代時細胞数 | split ratio = 1/5 |
| 人種 | 炭酸ガス濃度 | 5% | |
| 採取組織名 | esophagus | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:21044961 | MicroRNA-210 regulates cancer cell proliferation through targeting fibroblast growth factor receptor-like 1 (FGFRL1). Tsuchiya S,Fujiwara T,Sato F,Shimada Y,Tanaka E,Sakai Y,Shimizu K,Tsujimoto G J Biol Chem. 2011 Jan 7;286(1):420-8 |
| Pubmed id:16951226 | The biological role of the low-affinity p75 neurotrophin receptor in esophageal squamous cell carcinoma. Okumura T,Tsunoda S,Mori Y,Ito T,Kikuchi K,Wang TC,Yasumoto S,Shimada Y Clin Cancer Res. 2006 Sep 1;12(17):5096-103 |
| Pubmed id:17671398 | ABCG2 expression is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma. Tsunoda S,Okumura T,Ito T,Kondo K,Ortiz C,Tanaka E,Watanabe G,Itami A,Sakai Y,Shimada Y Oncology. 2006;71(3-4):251-8 |
| Pubmed id:15814639 | Prognostic significance of fascin overexpression in human esophageal squamous cell carcinoma. Hashimoto Y,Ito T,Inoue H,Okumura T,Tanaka E,Tsunoda S,Higashiyama M,Watanabe G,Imamura M,Shimada Y Clin Cancer Res. 2005 Apr 1;11(7):2597-605 |
| Pubmed id:12538476 | Cell culture in esophageal squamous cell carcinoma and the association with molecular markers. Shimada Y,Maeda M,Watanabe G,Yamasaki S,Komoto I,Kaganoi J,Kan T,Hashimoto Y,Imoto I,Inazawa J,Imamura M Clin Cancer Res. 2003 Jan;9(1):243-9 |
| Pubmed id:9033652 | Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines. Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K Int J Cancer. 1997 Feb 7;70(4):437-42 |
| Pubmed id:8575860 | Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K Int J Cancer. 1996 Jan 26;65(3):372-6 |
| Pubmed id:7913084 | Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M Int J Cancer. 1994 Jul 15;58(2):291-7 |
| Pubmed id:1728357 | Characterization of 21 newly established esophageal cancer cell lines. Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T Cancer. 1992 Jan 15;69(2):277-84 |
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LOT Information
| 細胞番号 | JCRB1426 | 細胞名 | KYSE190 |
|---|---|---|---|
| 培養ロット番号 | 09092011 | 培養種別 | distribution |
| 培地 | RPMI1640(GIBCO) and Ham's F12 medium(SIGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 0.24-1.0x10^5 cells/sq.cm | 継代方法 | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 3.6x10^6 |
| 凍結時生細胞率 | 91.1 | 使用抗生物質 | free |
| 継代数 | p4* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | D5S818:10 D13S317:11 D7S820:10,12 D16S539:9 VWA:16 TH01:6 AM:X TPOX:11 CSF1PO:11 |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Cell Banker BLC-1(Nihon Zenyaku Industries) | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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| 細胞番号 | JCRB1426 | 細胞名 | KYSE190 |
|---|---|---|---|
| 培養ロット番号 | 12172018 | 培養種別 | distribution |
| 培地 | 1:1 mixture of RPMI 1640 medium and Ham's F12 medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 0.5 - 1 x 10^5 cells/mL | 継代方法 | Cells were harvested after treatment wth 0.25% trypsin and 0.02% EDTA. |
| 増殖速度 | approx. 31 hrs. | 凍結時生細胞濃度 | 2.4 x 10^6 |
| 凍結時生細胞率 | 96.0 | 使用抗生物質 | free |
| 継代数 | Unknown (7 at bank) | PDL数（プライマリ） | |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | |
| 染色体モード | 染色体情報 | ||
| 表面抗原 | DNA Profile (STR) | ||
| 接着性 | Yes | 導入外部遺伝子 | |
| 凍結培地 | 10% DMSO, 20% FBS - RPMI1640/F12 | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 89.6 | 追加情報 |