JCRB1430 KYSE450


Important Notice(s)



Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB1430 細胞名   KYSE450
生物種(日本語)   ヒト 組織名(日本語)   食道
コメント(日本語)   扁平上皮がん, 高分化型, (民間企業利用者は、分譲依頼前に細胞樹立者とMTAを手交する必要があります。) プロフィール   Human well differentiated squamous cell carcinoma cell line established from esophageal cancer.
別名    動物名   human
系統名    学名・属名   Homo
種名   sapiens 性別   M
年齢・月齢   59 細胞識別情報   available
(癌)原発組織名   esophagus 病歴情報   well differentiated squamous cell carcinoma, stage 2b
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質    細胞寿命   infinite
クライシスPDL    形態   epithelial-like
一般性状   T1M1N0 stage 2b 細胞分類   tumor
細胞樹立者名   Shimada, Y. 細胞寄託者   Shimada, Y.
分譲時制限    コメント   
入手年   2011 培養培地   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum.
継代方法   Cells harvested after treatment with trypsin and EDTA. 継代時細胞数   split ratio = 1/5
人種    炭酸ガス濃度   5%
採取組織名   esophagus 組織型   

ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR)
tested ウイルスRNA
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy)はneXtProtプロジェクトの一環として、SIB - Swiss Institute of BioinformaticsのCALIPHOグループのAmos Bairoch博士によって開発されました。Cellosaurusは細胞株に関する様々な情報を集約させたデータベースになっています。JCRB細胞バンクに登録されている細胞株については、右のリンクから情報を得ることができます。 CVCL_1353
Pubmed id:17935131 Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma and its clinical significance.
Ortiz CM,Ito T,Tanaka E,Tsunoda S,Nagayama S,Sakai Y,Higashitsuji H,Fujita J,Shimada Y
Int J Cancer. 2008 Jan 15;122(2):325-32
Pubmed id:17671398 ABCG2 expression is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma.
Tsunoda S,Okumura T,Ito T,Kondo K,Ortiz C,Tanaka E,Watanabe G,Itami A,Sakai Y,Shimada Y
Oncology. 2006;71(3-4):251-8
Pubmed id:16951226 The biological role of the low-affinity p75 neurotrophin receptor in esophageal squamous cell carcinoma.
Okumura T,Tsunoda S,Mori Y,Ito T,Kikuchi K,Wang TC,Yasumoto S,Shimada Y
Clin Cancer Res. 2006 Sep 1;12(17):5096-103
Pubmed id:16003725 Cisplatin-dependent upregulation of death receptors 4 and 5 augments induction of apoptosis by TNF-related apoptosis-inducing ligand against esophageal squamous cell carcinoma.
Kondo K,Yamasaki S,Sugie T,Teratani N,Kan T,Imamura M,Shimada Y
Int J Cancer. 2006 Jan 1;118(1):230-42
Pubmed id:15814639 Prognostic significance of fascin overexpression in human esophageal squamous cell carcinoma.
Hashimoto Y,Ito T,Inoue H,Okumura T,Tanaka E,Tsunoda S,Higashiyama M,Watanabe G,Imamura M,Shimada Y
Clin Cancer Res. 2005 Apr 1;11(7):2597-605
Pubmed id:14522385 Growth inhibition through activation of peroxisome proliferator-activated receptor gamma in human oesophageal squamous cell carcinoma.
Hashimoto Y,Shimada Y,Itami A,Ito T,Kawamura J,Kawabe A,Kaganoi J,Maeda M,Watanabe G,Imamura M
Eur J Cancer. 2003 Oct;39(15):2239-46
Pubmed id:12538476 Cell culture in esophageal squamous cell carcinoma and the association with molecular markers.
Shimada Y,Maeda M,Watanabe G,Yamasaki S,Komoto I,Kaganoi J,Kan T,Hashimoto Y,Imoto I,Inazawa J,Imamura M
Clin Cancer Res. 2003 Jan;9(1):243-9
Pubmed id:9033652 Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860 Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:7913084 Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M
Int J Cancer. 1994 Jul 15;58(2):291-7
Pubmed id:1728357 Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84


LOT Information

細胞番号   JCRB1430 細胞名   KYSE450
培養ロット番号   10282011 培養種別   distribution
培地   RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) 培養温度   37 C
継代時細胞数(濃度)   1.33-1.97x10^4cells/sq.cm 継代方法   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
増殖速度   NT 凍結時生細胞濃度   3.03x10^6
凍結時生細胞率   94.8 使用抗生物質   free
継代数   p4* PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:10,13
接着性   Yes 導入外部遺伝子   NT
凍結培地   Cell Banker BLC-1(Nihon Zenyaku Industries) 炭酸ガス濃度   5 %
RFPL所見   NT 解凍後生細胞率