JCRB1564 TMNK-1
細胞情報
Important Notice(s)
This cell line is available for only academic users.
Endothelial marker expressions in TMNK-1 cells.
Articles regarding TMNK-1 / TWNT-1 / MMNK-1
About the cultivation of TMNK-1 cells.
細胞種類：遺伝子改変細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB1564 | 細胞名 | TMNK-1 |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 肝臓 |
| コメント(日本語) | 不死化ヒト肝内皮細胞(指定培地が特殊ですのでご注意ください) | プロフィール | Immortalized human-liver endothelial cell line with SV40T and hTERT. (Note: recommended medium is very specific.) |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | |
| 年齢・月齢 | 細胞識別情報 | available | |
| （癌）原発組織名 | 病歴情報 | ||
| 転移の有無（Y/N） | No | （癌）転移組織名 | |
| 遺伝的性質 | Immortalized by SV40T and hTERT. | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | epithelial-like | |
| 一般性状 | Expressed factor VIII, VEGF receptors (flt-1, KDR/Flk-1), and CD34. | 細胞分類 | transformed |
| 細胞樹立者名 | Kobayashi, N. | 細胞寄託者 | Mochitate, K. |
| 分譲時制限 | [Matsumura et al., Transplantation 77: 1357-1365, 2004] should be cited at the paper submission. | コメント | Distribution restricted to academic Japan domestic user, or only academic oversea users. The following reference should be cited at the publication of paper. Matsumura et al. Transplantation 77: 1357-1365, 2004. |
| 入手年 | 2013 | 培養培地 | Mochitate modified MCDB-131 medium with 50% of EGM-2 SingleQuot Kit (clontics #CC-4176; ascorbic acid was substituted by ascorbic acid phosphate magnesium salt n-Hydrate (0.2 mM)) |
| 継代方法 | Cells were harvested after treatment with accutase (Innovative cell technologies) and split ratio=1/3-1/4 | 継代時細胞数 | 0.3-1.5x10^5 cells/sq.cm (spli |
| 人種 | 炭酸ガス濃度 | 5% | |
| 採取組織名 | liver | 組織型 | liver endothelial cell |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15167590 | Establishment of an immortalized human-liver endothelial cell line with SV40T and hTERT. Matsumura T,Takesue M,Westerman KA,Okitsu T,Sakaguchi M,Fukazawa T,Totsugawa T,Noguchi H,Yamamoto S,Stolz DB,Tanaka N,Leboulch P,Kobayashi N Transplantation. 2004 May 15;77(9):1357-65 |
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LOT Information
| 細胞番号 | JCRB1564 | 細胞名 | TMNK-1 |
|---|---|---|---|
| 培養ロット番号 | 03042014 | 培養種別 | distribution |
| 培地 | Mochitate modified MCDB(Nissui) with 50% of EGM-2 SingleQuot Kit(Clonetics #CC-4176) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 0.3-1.5x10^5 cells/sq.cm | 継代方法 | Cells were harvested after treatment with accutase(Innovative cell technologies) and split ratio=1/3-1/4 |
| 増殖速度 | NT | 凍結時生細胞濃度 | 3.13x10^6 |
| 凍結時生細胞率 | 93.9 | 使用抗生物質 | |
| 継代数 | p20 | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | D5S818:11 D13S317:11 D7S820:11,13 D16S539:10,11 VWA:15,16 TH01:6,7 AM:X TPOX:9 CSF1PO:10 |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Mochitate modified MCDB with 100% of EGM-2 SingleQuot Kit and 10% DMSO | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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| 細胞番号 | JCRB1564 | 細胞名 | TMNK-1 |
|---|---|---|---|
| 培養ロット番号 | 04132018 | 培養種別 | distribution |
| 培地 | Mochitate modified MCDB131(PEPTIDE Institute Inc.;custom order) with 50% of EGM-2 SingleQuot Kit(Clonetics #CC-4176) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 2-4x10^4 cells/sq.cm | 継代方法 | Cells were harvested after treatment with accutase (Innovative cell technologies) and split ratio=1/3-1/4 |
| 増殖速度 | NT | 凍結時生細胞濃度 | 1.09x10^6 |
| 凍結時生細胞率 | 95.7 | 使用抗生物質 | free |
| 継代数 | p22 | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | D5S818:11 D13S317:11 D7S820:11,13 D16S539:10,11 VWA:15,16 TH01:6,7 AM:X TPOX:9 CSF1PO:10 |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Mochitate modified MCDB131 with 100% of EGM-2 SingleQuot Kit and 10% DMSO | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | NT | 追加情報 | Mycoplasma elimination by MC-210 treatment. 1vial is 0.5ml. |
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