JCRB4021 KMS21

細胞情報

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB4021 細胞名   KMS21
生物種(日本語)   ヒト 組織名(日本語)   血球・リンパ系
コメント(日本語)   骨髄腫, 骨髄より樹立, KMS21R、KMS21RFとセットで寄託 プロフィール   human myeloma cell line. Deposited as a set with KMS21R and KMS21RF
別名   KMS-21, KMS-21BM 動物名   human
系統名    学名・属名   Homo
種名   sapiens 性別   M
年齢・月齢   62-year-old 細胞識別情報   available
(癌)原発組織名    病歴情報   hyperammonaemia
転移の有無(Y/N)   Y (癌)転移組織名   
遺伝的性質   t(11;14)(q13;q32) 細胞寿命   infinite
クライシスPDL    形態   hemocyte-like
一般性状   small round-cell morphology, part is multinucleate cell. 細胞分類   tumor
細胞樹立者名   Takemi OTSUKI 細胞寄託者   Yutaka HATTORI
分譲時制限    コメント   
入手年    培養培地   RPMI1640 medium with 10% featal bovine serum.
継代方法   Subculture twice a week by dilution. 継代時細胞数   50000 cells / mL
人種   Japanese 炭酸ガス濃度   5%
採取組織名   bone marrow 組織型   

ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR)
ウイルスDNA
検出検査
tested ウイルスRNA
検出検査
not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:11697507Establishment of CD7+ human myeloma sister cell lines, KMS-21-PE and KMS-21-BM, carrying t(11;14) and t(8;14).
Otsuki T,Wada H,Nakazawa N,Taniwaki M,Kouguchi K,Ohkura M,Uehira K,Isoda K,Yata K,Sakaguchi H,Yawata Y,Ueki A,Yamada O
Leuk Lymphoma. 2001 Aug;42(4):761-74
Pubmed id:11039672Cellular biological differences between human myeloma cell lines KMS-12-PE and KMS-12-BM established from a single patient.
Otsuki T,Hata H,Harada N,Matsuzaki H,Yata K,Wada H,Yawata Y,Ueki A,Yamada O
Int J Hematol. 2000 Aug;72(2):216-22


Research results by users. Click!
Pubmed id:30297108 A novel derivative (GTN024) from a natural product, komaroviquinone, induced the apoptosis of high-risk myeloma cells via reactive oxygen production and ER stress.
Okayama M,Kitabatake S,Sato M,Fujimori K,Ichikawa D,Matsushita M,Suto Y,Iwasaki G,Yamada T,Kiuchi F,Hirao M,Kunieda H,Osada M,Okamoto S,Hattori Y
Biochem Biophys Res Commun. 2018 Nov 2;505(3):787-793
Pubmed id:28867196 A novel phenylphthalimide derivative, pegylated TC11, improves pharmacokinetic properties and induces apoptosis of high-risk myeloma cells via G2/M cell-cycle arrest.
Aida S,Hozumi M,Ichikawa D,Iida K,Yonemura Y,Tabata N,Yamada T,Matsushita M,Sugai T,Yanagawa H,Hattori Y
Biochem Biophys Res Commun. 2017 Nov 4;493(1):514-520
Pubmed id:28882484 Synthesis and biological evaluation of the natural product komaroviquinone and related compounds aiming at a potential therapeutic lead compound for high-risk multiple myeloma.
Suto Y,Sato M,Fujimori K,Kitabatake S,Okayama M,Ichikawa D,Matsushita M,Yamagiwa N,Iwasaki G,Kiuchi F,Hattori Y
Bioorg Med Chem Lett. 2017 Oct 1;27(19):4558-4563

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LOT Information

細胞番号   JCRB4021 細胞名   KMS21
培養ロット番号   04252024 培養種別   distribution
培地   RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) 培養温度   37 C
継代時細胞数(濃度)   0.767-7.9x10^5 cells/sq.cm 継代方法   Dilution, twice a week
増殖速度   NT 凍結時生細胞濃度   2.47x10^6
凍結時生細胞率   72.76 使用抗生物質   free
継代数   p13 PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,14
D7S820:8,13
D16S539:9
VWA:18
TH01:7,9
AM:X
TPOX:8
CSF1PO:12,13
接着性   No 導入外部遺伝子   NT
凍結培地   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) 炭酸ガス濃度   5%
解凍後生細胞率   NT 追加情報   
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