IFO50419 ASF-4-2
Cell information
Important Notice(s)ASF-4シリーズ線維芽細胞の使用目的の限定について / The limitation of use for ASF-4 series fibroblasts
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50419 | Cell Name | ASF-4-2 |
|---|---|---|---|
| Profile | Normal diploid (46, XY) fibroblast with limited life span. The ASF-4-1 and ASF-4-2 lines were established from skins of the same donor at different ages. This cell line was taken from the donor at 47 years and 6 month-old. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 47.5 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | finite | Crisis PDL | 62.1 |
| Morphology | Character | skin normal diploid fibroblast | |
| Classify | primary | Established by | Kaji, K. |
| Registered by | Kaji,K. | Regulation for Distribution | Limited to the research for scenescence / rejuvenation. Developer's approval is required. |
| Comment | Year | 1993 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | trypsin |
| Cell Number on Passage | ca 1 x 10^4 cells/cm2, weekly | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | skin fibroblast from upper arm |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
| Cell No. | IFO50419 | Cell Name | |
|---|---|---|---|
| LOT No. | 08062002 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | 3-6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | D.T. = ca 60 hrs | Cell Number in Vial (cells/1ml) | 4.9 x 10^5 |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | P6 at bank | PDL | 30 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50419 | Cell Name | ASF-4-2 |
|---|---|---|---|
| LOT No. | 848 | Lot Specification | seed/distribution |
| Medium | E.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 5 x 10^5 | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unknown (2 at IFO) | PDL | 22 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | not tested |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:9,10 D13S317:11,12 D7S820:10,11 D16S539:10,11 VWA:17,18 TH01:8,9 AM:XY TPOX:11 CSF1PO:10,13 |
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| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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