JCRB0302 XP2YO(SV)
Cell information
Important Notice(s)Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0302 | Cell Name | XP2YO(SV) |
|---|---|---|---|
| Profile | xeroderma pigmentosum (F), SV40 transformed | Other Name | XP |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Xeroderma pigmentosum (F), SV40 transformed | |
| Life Span | Crisis PDL | ||
| Morphology | Character | Xeroderma pigmentosum (F), SV40 transformed | |
| Classify | Established by | Takebe,H. | |
| Registered by | Takebe,H. | Regulation for Distribution | |
| Comment | Hereditary deisease with high cancer risk,xeroderma pigmentosum (autosomal recessive),complementation group F,SV40-transformed skin fibroblast. | Year | 1985 |
| Medium | alfa-MEM, 10% heat-inactivated fetal bovine serum. (establish: Dulbecco's modified Eagle's medium with 10% fetal calf serum) | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25% trypsin. |
| Cell Number on Passage | split ratio=1/3 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:6298614 | Establishment by SV40 transformation and characteristics of a cell line of xeroderma pigmentosum belonging to complementation group F. Yagi T,Takebe H Mutat Res. 1983 Feb;112(1):59-66 |
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LOT Information
| Cell No. | JCRB0302 | Cell Name | XP2YO(SV) |
|---|---|---|---|
| LOT No. | 04182008 | Lot Specification | distribution |
| Medium | DMEM with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | NT | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.55x10^6 |
| Viability at cell freezing (%) | 97.9 | Antibiotics Used | NT |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13 D13S317:8,10 D7S820:11,12 D16S539:11 VWA:13,17 TH01:9 AM:X TPOX:8 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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