JCRB1442 ASF-4-4L2
Cell information
Important Notice(s)ASF-4シリーズ線維芽細胞の使用目的の限定について / The limitation of use for ASF-4 series fibroblasts
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB1442 | Cell Name | ASF-4-4L2 |
---|---|---|---|
Profile | Normal diploid fibroblast with limeted life span. The ASF-4-1, ASF-4-2, ASF-4-3 and the ASF-4-4 lines were established from skins of the same donor at different ages. | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | M | Age | 62.6-year-old |
Identity | available | Tissue for Primary Cancer | |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | skin fibroblast, diploid (M) | |
Life Span | finite | Crisis PDL | 48.4 PDL |
Morphology | fibroblast-like | Character | normal fibroblast |
Classify | primary | Established by | Kaji, K. |
Registered by | Kaji, K. | Regulation for Distribution | Limited to the research for scenescence / rejuvenation. Developer's approval is required. |
Comment | Year | 2010 | |
Medium | Eagle's minimal essential medium with 10% fetal bovine serum. | Methods for Passages | Subculture every 7 days. Split ratio, 1/4. 0.25% trypsin, 10-15 min. |
Cell Number on Passage | ≃ 1x10^4 cells/cm^2 | Race | Japanese |
CO2 Conc. | 5% | Tissue Sampling | skin |
Tissue Type | skin |
Detection of virus genome fragment by Real-time PCR | |||||||||
---|---|---|---|---|---|---|---|---|---|
Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Images |
---|