JCRB3026 AP38P(SVT)
Cell information
Cell type:human mutant cell repository from Kyoto University (View Pricing Information)
JCRB No. | JCRB3026 | Cell Name | AP38P(SVT) |
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Profile | Hereditary disease with high cancer risk Fanconi anemia (autosomal recessive) chromosomal breakage syndrome high sensitivity to mitomycin C | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | F | Age | 9-year-old |
Identity | available | Tissue for Primary Cancer | |
Case history | Fanconi anemia (A) | Metastasis | |
Tissue Metastasized | Genetics | MMC sensitivity(-)(post bone marrow transplant lymphocyte), FANCA mutation | |
Life Span | Crisis PDL | ||
Morphology | fibroblast-like | Character | SV40 Ori-Transfection |
Classify | transformed | Established by | |
Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
Comment | Because of bone marrow transplantation MMC sensitivity in lymphocyte is normal. identical twin of AP38S | Year | |
Medium | Methods for Passages | ||
Cell Number on Passage | Race | Japanese | |
CO2 Conc. | 5% | Tissue Sampling | skin |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
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LOT Information
Cell No. | JCRB3026 | Cell Name | AP38P(SVT) |
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LOT No. | 06122015 | Lot Specification | seed/distribution |
Medium | MEM alpha (GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
Cell Density at Seeding | 0.53-1.7x10^5 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.40x10^6 |
Viability at cell freezing (%) | 93.0 | Antibiotics Used | free |
Passage Number | p3 | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:9 D7S820:10,11 D16S539:11,14 VWA:15,17 TH01:6,9 AM:X TPOX:8,11 CSF1PO:12,15 |
Adhesion | Yes | Exoteric Gene | NT |
Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
Viability immediately after thawing (%) | Additional information |
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