-Detailed Information [JCRB0403]-

JCRB0403 HuH-7

Cell information

Important Notice(s)

Tips for the cultivation of JCRB0403 HuH-7

[Tips for the cultivation of JCRB0403 HuH-7]

!! Important !!

[Medium]

The DMEM (Dulbecco's modified Eagle's medium) used for HuH-7 is low glucose DMEM. The standard DMEM is low glucose formulation, and high glucose DMEM is a modification.
DO NOT use high glucose DMEM. Many troubles have been reported caused by the use of high glucose DMEM in the HuH-7 culture.
Standard DMEM contains L-glutamine, sodium pyruvate, and low glucose (1 g/L). HEPES should not be included in DMEM.

[Size of flask (dish) at the initiation of culture]

The survival of HuH-7 cells immediately after thawing is dependent on cell density. Please seed all cells in vial to one T-25 flask or one 6 cm dish.
DMSO should be removed before seeding by brief centrifugation.
Too much dilution of cells at re-cultivation and passaging may cause cell death or retardation of growth. 

[Passaging]

In trypsinization of cells, please treat them enough amount of trypsin solution and the cells should be collected by brief centrifugation. The treatment of less amount of trypsin solution may cause dry-up of cells and may damage the cells.



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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0403	Cell Name	HuH-7
Profile	Well differentiated human hepato cellular carcinoma cell line.	Other Name	HuH-7, JTC-39
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	57
Identity	available	Tissue for Primary Cancer	liver, gallbladder
Case history	Well differentiated hepato cellular carcinoma.	Metastasis
Tissue Metastasized		Genetics	differenciated hepatoma
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	hepatoma (differenciated), age 57, (M)
Classify	tumor	Established by	Nakabayshi,H. & Sato,J.
Registered by	Sato,J.	Regulation for Distribution
Comment	This cell line can be grown in serum-free medium. Many serum proteins with alpha fetoprotein are released into the medium.	Year	1985
Medium	Dulbecco's minimal essential medium with 10% fetal bovine serum. Refer Ref {1201} for other medium with low serum concentration.	Methods for Passages	Cells are treated with 0.2% trypsin and 0.02% EDTA.
Cell Number on Passage	2.5x10^4 cells/cm^2	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	liver
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0336
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	907071

Reference
Pubmed id:29774518	HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity. Kasai F,Hirayama N,Ozawa M,Satoh M,Kohara A Hum Cell. 2018 Jul;31(3):261-267
Pubmed id:12518066	Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs. Randall G,Grakoui A,Rice CM Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):235-40
Pubmed id:12438626	Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication. Blight KJ,McKeating JA,Rice CM J Virol. 2002 Dec;76(24):13001-14
Pubmed id:10390360	Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Lohmann V,Körner F,Koch J,Herian U,Theilmann L,Bartenschlager R Science. 1999 Jul 2;285(5424):110-3
Pubmed id:10576351	Establishment and characteristics of human hepatocellular carcinoma cells with metastasis to lymph nodes. Seki S,Kitada T,Kawada N,Sakaguchi H,Kadoya H,Nakatani K,Satake K,Kuroki T Hepatogastroenterology. 1999 Sep-Oct;46(29):2812-7
Pubmed id:2415243	Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium. Nakabayashi H,Taketa K,Yamane T,Oda M,Sato J Cancer Res. 1985 Dec;45(12 Pt 1):6379-83
Pubmed id:6203805	Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium. Nakabayashi H,Taketa K,Yamane T,Miyazaki M,Miyano K,Sato J Gan. 1984 Feb;75(2):151-8
Pubmed id:6286115	Growth of human hepatoma cells lines with differentiated functions in chemically defined medium. Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J Cancer Res. 1982 Sep;42(9):3858-63

Images

Movies

LOT Information

03192012
07152011
03282011
08192010
08062010
02152010
03032009
02272008
07112007
07112005
10022003
03272002
03292000
102296
021396
122597
052599
04232013
10312014
06222015
04182016
07222016
04172017
08282017
10192018
11292019
12202019
07172020
06042020
03032021
09222021
12172021
02092022
03182022
04192022
09202022
03032023
12162022
10262023
10272023

Cell No.	JCRB0403	Cell Name
LOT No.	03192012	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (containing 4 mM L-glutamine and 1 g/L glucose) with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	5.4 - 7.2 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time	approx. 37 hrs.	Cell Number in Vial (cells/1ml)	9.3 x 10^5
Viability at cell freezing (%)	97.5	Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS -DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	07152011	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (containing 4 mM L-glutamine) with 10% fetal bovine serum (GIBCO Cat. # 10099).	Temperature	37 C
Cell Density at Seeding	5.9 - 7.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time	approx. 33 hrs	Cell Number in Vial (cells/1ml)	5.4 x 10^5
Viability at cell freezing (%)	87	Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	03282011	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10099).	Temperature	37 C
Cell Density at Seeding	approx. 5 x 10^4 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time	approx. 31 hrs	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	96.5	Antibiotics Used	free
Passage Number	P52	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	08192010	Lot Specification	distribution
Medium	DMEM with 10% heat inactivated fetal bovine serum (SIGMA027K03911). DMEM must contain L-glutamine, and the glucose concentration is 1 g/L (low glucose type is the standard of DMEM).	Temperature	37 C
Cell Density at Seeding	1-4x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO). 1/4-1/8 split once a week .
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.57x10^6
Viability at cell freezing (%)	87.4	Antibiotics Used	free
Passage Number	p49	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	08062010	Lot Specification	distribution
Medium	DMEM (low glucose type: 1 g/L glucose) with 10% heat inactivated fetal bovine serum(SIGMA027K03911)	Temperature	37 C
Cell Density at Seeding	1-4x10^4 cells/sq.cm	Methods for Passages	All ampoule contents should be sheeded to one T-25 flask after the removal of DMSO by brief centribugation at the thawing. Cells were harvested after treatment with 0.25%trypsin(GIBCO). 1/4-1/8 split once a week .
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.27x10^6
Viability at cell freezing (%)	96.3	Antibiotics Used	free
Passage Number	p47	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0403	Cell Name
LOT No.	02152010	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	5 - 6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time	approx. 1.5 days	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	95.8	Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	03032009	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375)	Temperature	37 C
Cell Density at Seeding	3 - 10 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time	approx. 1.5 days	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	94.3	Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	02272008	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.#10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	3 - 10 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	30 hrs	Cell Number in Vial (cells/1ml)	2.2 x 10^6
Viability at cell freezing (%)	97	Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	07112007	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) [Do not use Fungizone (Amphotericin B). It may result retardation of growth.]	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 2-3 days	Cell Number in Vial (cells/1ml)	2.3 x 10^6
Viability at cell freezing (%)	98.3	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	07112005	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	4 - 9 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	97.2	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	10022003	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S)	Temperature	37 C
Cell Density at Seeding	5-10 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T. = ca. 2 days	Cell Number in Vial (cells/1ml)	1.8 x 10^6
Viability at cell freezing (%)	90.5	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	03272002	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	5-10 x 10^4 cells/ml	Methods for Passages	0.2% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time	D.T. = ca 50 hrs	Cell Number in Vial (cells/1ml)	9.3 x 10^5
Viability at cell freezing (%)	97	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FCS-DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	03292000	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal calf serum	Temperature	37 C
Cell Density at Seeding	3.0 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	Slow	Cell Number in Vial (cells/1ml)	3.8 x 10^6
Viability at cell freezing (%)		Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FCS-DMEM	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	102296	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum (JRS DO10400)	Temperature	37 C
Cell Density at Seeding	2.2 x 10^5 cells/ml	Methods for Passages	Wash cells with PBS(-) once and treat cells with 0.1% trypsin and 0.02% EDTA for 5 min. at 37 C.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.7x10^6
Viability at cell freezing (%)	91.0	Antibiotics Used	free
Passage Number	P49	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(type B), LDH, NP. (human)
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	021396	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum.	Temperature	37 C
Cell Density at Seeding	2.5x10^4 cells/ml	Methods for Passages	Cells rinsed with PBS(-) and treated with 0.1% trypsin and 0.005% EDTA at 37 C.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.9x10^6
Viability at cell freezing (%)	80.2	Antibiotics Used	free
Passage Number	P44	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	122597	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	5-10 x 10^4 cells/ml	Methods for Passages	0.2% trypsin and 0.02% EDTA
Doubling Time	about 4 days	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	95.0	Antibiotics Used	free
Passage Number	P55	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name
LOT No.	052599	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	1.0 x 10^5 cells/ml	Methods for Passages	0.2% trypsin and 0.02% EDTA
Doubling Time	D.T.=ca. 55 hrs	Cell Number in Vial (cells/1ml)	7.5 x 10^5
Viability at cell freezing (%)	90.0	Antibiotics Used	free
Passage Number	P55	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, MD, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	04232013	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099).	Temperature	37 C
Cell Density at Seeding	5 - 10 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.（ロット番号誤表記:02232013と記載, Ampoule mislabel: lot# 02232013）
Doubling Time		Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	94.6	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	10312014	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. Use T25 flask or smaller one (larger flask will carry a risk of low viability)	Temperature	37 C
Cell Density at Seeding	approx. 6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	2.6 x 10^6
Viability at cell freezing (%)	87.5	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	87.5	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	06222015	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM.	Temperature	37 C
Cell Density at Seeding	4.8 - 7.8 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 2.5 days	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS -DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	95	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	04182016	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM.	Temperature
Cell Density at Seeding	5 - 7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.2% trypsin and 0.02% EDTA.
Doubling Time	approx. 1.5 day	Cell Number in Vial (cells/1ml)	1.9 x 106
Viability at cell freezing (%)	90.7	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	90.7	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	07222016	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM.	Temperature	37 C
Cell Density at Seeding	5.7 - 6.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 52 hrs.	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	92.7	Antibiotics Used	free
Passage Number	P48	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	92.7	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	04172017	Lot Specification	distribution
Medium	DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396)	Temperature	37 C
Cell Density at Seeding	0.998-1.5x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.15x10^6
Viability at cell freezing (%)	91	Antibiotics Used	free
Passage Number	p48	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	84	Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	08282017	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012).	Temperature	37 C
Cell Density at Seeding	3.3 - 5.7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 53 hrs.	Cell Number in Vial (cells/1ml)	9.4 x 10^5
Viability at cell freezing (%)	92.2	Antibiotics Used	free
Passage Number	P47	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	92.2	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	10192018	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012).	Temperature	37 C
Cell Density at Seeding	0.7 - 1.3 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 2 days.	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	93.3	Antibiotics Used	free
Passage Number	P51	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	93.3	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	11292019	Lot Specification	distribution
Medium	DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396)	Temperature	37 C
Cell Density at Seeding	1.0-1.5x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/5)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.5x10^6
Viability at cell freezing (%)	97.20	Antibiotics Used	free
Passage Number	p51	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	97.7	Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	12202019	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012).	Temperature	37 C
Cell Density at Seeding	3.0 - 5.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 58 hrs.	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	81.0	Antibiotics Used	free
Passage Number	P50	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	82.3	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	07172020	Lot Specification	distribution
Medium	DMEM (GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396)	Temperature	37 C
Cell Density at Seeding	1.0-1.7x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(1/5 split)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.5x10^6
Viability at cell freezing (%)	98.00	Antibiotics Used	free
Passage Number	p51	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	93.5	Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	06042020	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) and 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.5 - 1.9 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	2.1 x 10^6
Viability at cell freezing (%)	96.9	Antibiotics Used	free
Passage Number	P51	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	96.8	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	03032021	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) and 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.3 - 1.7 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	1.3 x 10^6
Viability at cell freezing (%)	98.3	Antibiotics Used	free
Passage Number	P51	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	95.4	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	09222021	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	2.3 - 4.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	6.9 x 10^5
Viability at cell freezing (%)	93	Antibiotics Used	free
Passage Number	P51	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	90	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	12172021	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	5.6 - 6.7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx 2.5 days	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	92.5	Antibiotics Used	free
Passage Number	P52	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	92.0	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	02092022	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum((SIGMA,Cat#172012,Lot#12J396))	Temperature	37 C
Cell Density at Seeding	1.3-1.5x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/4-1/6)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.0x10^6
Viability at cell freezing (%)	97.00	Antibiotics Used	free
Passage Number	p52	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	81.6	Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	03182022	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum((SIGMA,Cat#172012,Lot#12J396))	Temperature	37 C
Cell Density at Seeding	1.3-2.4x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(1/4-1/6 split)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.0x10^6
Viability at cell freezing (%)	97.90	Antibiotics Used	free
Passage Number	p51	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	98	Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	04192022	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	7.6 - 8.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 43 hrs.	Cell Number in Vial (cells/1ml)	1.8 x 10^6
Viability at cell freezing (%)	97	Antibiotics Used	free
Passage Number	P54	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	09202022	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	2.6 - 6.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 3 days	Cell Number in Vial (cells/1ml)	2.1 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	P52	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	91	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	03032023	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (FUJIFILM-WAKO Cat 041-29775）with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37C
Cell Density at Seeding	1.0-1.6 x 10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	95.8	Antibiotics Used	free
Passage Number	P52	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5％
Viability immediately after thawing (%)	81.9	Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	12162022	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322)	Temperature	37 C
Cell Density at Seeding	1 - 5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 40 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	95.7	Antibiotics Used	free
Passage Number	P50	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	92.0	Additional information

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	10262023	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (Nacalai Tesque #08456-65) with 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	1.1-1.7x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.38x10^6
Viability at cell freezing (%)	98.5	Antibiotics Used	free
Passage Number	p53	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	98	Additional information

Images

Cell No.	JCRB0403	Cell Name	HuH-7
LOT No.	10272023	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (Nacalai Tesque #08456-65) with 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	1.1-1.7x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with0.25% trypsin and 1mM EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.76x10^6
Viability at cell freezing (%)	97.2	Antibiotics Used	free
Passage Number	p53	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:10,11 D7S820:11 D16S539:10 VWA:16,18 TH01:7 AM:X TPOX:8,11 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

Images

JCRB Cell Bank

JCRB0403 HuH-7

Cell information

LOT Information