JCRB0403 HuH-7

Cell information

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Tips for the cultivation of JCRB0403 HuH-7

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0403 Cell Name   HuH-7
Profile   Well differentiated human hepato cellular carcinoma cell line. Other Name   HuH-7, JTC-39
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   57
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   Well differentiated hepato cellular carcinoma. Metastasis   
Tissue Metastasized    Genetics   differenciated hepatoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   hepatoma (differenciated), age 57, (M)
Classify   tumor Established by   Nakabayshi,H. & Sato,J.
Registered by   Sato,J. Regulation for Distribution   
Comment   This cell line can be grown in serum-free medium. Many serum proteins with alpha fetoprotein are released into the medium. Year   1985
Medium   Dulbecco's minimal essential medium with 10% fetal bovine serum. Refer Ref {1201} for other medium with low serum concentration. Methods for Passages   Cells are treated with 0.2% trypsin and 0.02% EDTA.
Cell Number on Passage   2.5x10^4 cells/cm^2 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV1(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0336
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907071
Reference
Pubmed id:29774518 HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Kasai F,Hirayama N,Ozawa M,Satoh M,Kohara A
Hum Cell. 2018 Jul;31(3):261-267
Pubmed id:12518066 Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs.
Randall G,Grakoui A,Rice CM
Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):235-40
Pubmed id:12438626 Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication.
Blight KJ,McKeating JA,Rice CM
J Virol. 2002 Dec;76(24):13001-14
Pubmed id:10390360 Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.
Lohmann V,Körner F,Koch J,Herian U,Theilmann L,Bartenschlager R
Science. 1999 Jul 2;285(5424):110-3
Pubmed id:10576351 Establishment and characteristics of human hepatocellular carcinoma cells with metastasis to lymph nodes.
Seki S,Kitada T,Kawada N,Sakaguchi H,Kadoya H,Nakatani K,Satake K,Kuroki T
Hepatogastroenterology. 1999 Sep-Oct;46(29):2812-7
Pubmed id:2415243 Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium.
Nakabayashi H,Taketa K,Yamane T,Oda M,Sato J
Cancer Res. 1985 Dec;45(12 Pt 1):6379-83
Pubmed id:6203805 Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.
Nakabayashi H,Taketa K,Yamane T,Miyazaki M,Miyano K,Sato J
Gan. 1984 Feb;75(2):151-8
Pubmed id:6286115 Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.
Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J
Cancer Res. 1982 Sep;42(9):3858-63

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JCRB0403_04182016.flv



LOT Information

Cell No.   JCRB0403 Cell Name   
LOT No.   03192012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (containing 4 mM L-glutamine and 1 g/L glucose) with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5.4 - 7.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time   approx. 37 hrs. Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability (%)   97.5 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   07152011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (containing 4 mM L-glutamine) with 10% fetal bovine serum (GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   5.9 - 7.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time   approx. 33 hrs Cell Number in Vial (cells/1ml)   5.4 x 10^5
Viability (%)   87 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   03282011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability (%)   96.5 Antibiotics Used   free
Passage Number   P52 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   08192010 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum (SIGMA027K03911). DMEM must contain L-glutamine, and the glucose concentration is 1 g/L (low glucose type is the standard of DMEM). Temperature   37 C
Cell Density at Seeding   1-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO). 1/4-1/8 split once a week .
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.57x10^6
Viability (%)   87.4 Antibiotics Used   free
Passage Number   p49 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:10
VWA:16,18
TH01:7
AM:X
TPOX:8,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Additional information   
Images
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   08062010 Lot Specification   distribution
Medium   DMEM (low glucose type: 1 g/L glucose) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1-4x10^4 cells/sq.cm Methods for Passages   All ampoule contents should be sheeded to one T-25 flask after the removal of DMSO by brief centribugation at the thawing. Cells were harvested after treatment with 0.25%trypsin(GIBCO). 1/4-1/8 split once a week .
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.27x10^6
Viability (%)   96.3 Antibiotics Used   free
Passage Number   p47 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:10
VWA:16,18
TH01:7
AM:X
TPOX:8,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP    Additional information   
Images
Cell No.   JCRB0403 Cell Name   
LOT No.   02152010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5 - 6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability (%)   95.8 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   03032009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   3 - 10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability (%)   94.3 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   02272008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.#10099, lot 484375) Temperature   37 C
Cell Density at Seeding   3 - 10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   30 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability (%)   97 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   07112007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) [Do not use Fungizone (Amphotericin B). It may result retardation of growth.] Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2-3 days Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability (%)   98.3 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   07112005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   4 - 9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability (%)   97.2 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   10022003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 2 days Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability (%)   90.5 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   03272002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.2% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   D.T. = ca 50 hrs Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability (%)   97 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   03292000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   3.0 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   Slow Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability (%)    Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-DMEM CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   102296 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum (JRS DO10400) Temperature   37 C
Cell Density at Seeding   2.2 x 10^5 cells/ml Methods for Passages   Wash cells with PBS(-) once and treat cells with 0.1% trypsin and 0.02% EDTA for 5 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability (%)   91.0 Antibiotics Used   free
Passage Number   P49 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), LDH, NP. (human)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Images
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   021396 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum. Temperature   37 C
Cell Density at Seeding   2.5x10^4 cells/ml Methods for Passages   Cells rinsed with PBS(-) and treated with 0.1% trypsin and 0.005% EDTA at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.9x10^6
Viability (%)   80.2 Antibiotics Used   free
Passage Number   P44 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   122597 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.2% trypsin and 0.02% EDTA
Doubling Time   about 4 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability (%)   95.0 Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   
LOT No.   052599 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.0 x 10^5 cells/ml Methods for Passages   0.2% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca. 55 hrs Cell Number in Vial (cells/1ml)   7.5 x 10^5
Viability (%)   90.0 Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   04232013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.(ロット番号誤表記:02232013と記載, Ampoule mislabel: lot# 02232013)
Doubling Time    Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability (%)   94.6 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   10312014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. Use T25 flask or smaller one (larger flask will carry a risk of low viability) Temperature   37 C
Cell Density at Seeding   approx. 6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability (%)   87.5 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   06222015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. Temperature   37 C
Cell Density at Seeding   4.8 - 7.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2.5 days Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability (%)   95 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   04182016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. Temperature   
Cell Density at Seeding   5 - 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.2% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 day Cell Number in Vial (cells/1ml)   1.9 x 106
Viability (%)   90.7 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   07222016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. Temperature   37 C
Cell Density at Seeding   5.7 - 6.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 52 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability (%)   92.7 Antibiotics Used   free
Passage Number   P48 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   04172017 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.998-1.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.15x10^6
Viability (%)   91 Antibiotics Used   free
Passage Number   p48 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:10
VWA:16,18
TH01:7
AM:X
TPOX:8,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Additional information   
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