JCRB0403 HuH-7

Cell information

Important Notice(s)

Tips for the cultivation of JCRB0403 HuH-7

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0403 Cell Name   HuH-7
profile   Well differentiated human hepato cellular carcinoma cell line. Alias   HuH-7, JTC-39
animal   human strain   
genus   Homo species   sapiens
sex   M age   57
identity   available tissue   liver, gallbladder
case history   Well differentiated hepato cellular carcinoma. metastasis   
metastorgn    genetics   differenciated hepatoma
lifespan   infinite crisispdl   
morphology   epithelial-like charact   hepatoma (differenciated), age 57, (M)
classify   tumor originator   Nakabayshi,H. & Sato,J.
register   Sato,J. regulation   
comment   This cell line can be grown in serum-free medium. Many serum proteins with alpha fetoprotein are released into the medium. dyear   1985
medium   Dulbecco's minimal essential medium with 10% fetal bovine serum. Refer Ref {1201} for other medium with low serum concentration. pasmethod   Cells are treated with 0.2% trypsin and 0.02% EDTA.
pascellno   2.5x10^4 cells/cm^2 race   Japanese
co2conc   5 % tohroku   registered
preporgn   liver vrsummary   tested
vrsdetect   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV1(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] tissueform   


Link
COSMIC CELL LINE 907071
Reference
Pubmed id:12518066 Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs.
Randall G,Grakoui A,Rice CM
Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):235-40
Pubmed id:12438626 Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication.
Blight KJ,McKeating JA,Rice CM
J Virol. 2002 Dec;76(24):13001-14
Pubmed id:10390360 Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.
Lohmann V,Körner F,Koch J,Herian U,Theilmann L,Bartenschlager R
Science. 1999 Jul 2;285(5424):110-3
Pubmed id:10576351 Establishment and characteristics of human hepatocellular carcinoma cells with metastasis to lymph nodes.
Seki S,Kitada T,Kawada N,Sakaguchi H,Kadoya H,Nakatani K,Satake K,Kuroki T
Hepatogastroenterology. 1999 Sep-Oct;46(29):2812-7
Pubmed id:2415243 Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium.
Nakabayashi H,Taketa K,Yamane T,Oda M,Sato J
Cancer Res. 1985 Dec;45(12 Pt 1):6379-83
Pubmed id:6203805 Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.
Nakabayashi H,Taketa K,Yamane T,Miyazaki M,Miyano K,Sato J
Gan. 1984 Feb;75(2):151-8
Pubmed id:6286115 Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.
Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J
Cancer Res. 1982 Sep;42(9):3858-63

Images
Movies
JCRB0403_04182016.flv



LOT Information

Cell No.   JCRB0403 Cell Name   
LOT No.   03192012 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (containing 4 mM L-glutamine and 1 g/L glucose) with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) GROWTEMP   37 C
PASCELLNO   5.4 - 7.2 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
GROWTHRATE   approx. 37 hrs. FREEZCONC   9.3 x 10^5
VIABLFREEZ   97.5 ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS -DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   07152011 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (containing 4 mM L-glutamine) with 10% fetal bovine serum (GIBCO Cat. # 10099). GROWTEMP   37 C
PASCELLNO   5.9 - 7.4 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
GROWTHRATE   approx. 33 hrs FREEZCONC   5.4 x 10^5
VIABLFREEZ   87 ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   03282011 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10099). GROWTEMP   37 C
PASCELLNO   approx. 5 x 10^4 cells/mL PASMETHOD   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
GROWTHRATE   approx. 31 hrs FREEZCONC   1.7 x 10^6
VIABLFREEZ   96.5 ANTIBIOTIC   free
PASSAGE   P52 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   08192010 LOTSPEC   distribution
MEDIUM   DMEM with 10% heat inactivated fetal bovine serum (SIGMA027K03911). DMEM must contain L-glutamine, and the glucose concentration is 1 g/L (low glucose type is the standard of DMEM). GROWTEMP   37 C
PASCELLNO   1-4x10^4 cells/sq.cm PASMETHOD   Cells were harvested after treatment with 0.25%trypsin(GIBCO). 1/4-1/8 split once a week .
GROWTHRATE   NT FREEZCONC   3.57x10^6
VIABLFREEZ   87.4 ANTIBIOTIC   free
PASSAGE   p49 PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   NT
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:10
VWA:16,18
TH01:7
AM:X
TPOX:8,11
CSF1PO:11
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2   5 %
RFLP   NT FROMLOT   
Additional information   


Images
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   08062010 LOTSPEC   distribution
MEDIUM   DMEM (low glucose type: 1 g/L glucose) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) GROWTEMP   37 C
PASCELLNO   1-4x10^4 cells/sq.cm PASMETHOD   All ampoule contents should be sheeded to one T-25 flask after the removal of DMSO by brief centribugation at the thawing. Cells were harvested after treatment with 0.25%trypsin(GIBCO). 1/4-1/8 split once a week .
GROWTHRATE   NT FREEZCONC   2.27x10^6
VIABLFREEZ   96.3 ANTIBIOTIC   free
PASSAGE   p47 PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   NT
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:10
VWA:16,18
TH01:7
AM:X
TPOX:8,11
CSF1PO:11
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2   5 %
RFLP    FROMLOT   021296
Additional information   


Images
Cell No.   JCRB0403 Cell Name   
LOT No.   02152010 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) GROWTEMP   37 C
PASCELLNO   5 - 6 x 10^4 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
GROWTHRATE   approx. 1.5 days FREEZCONC   1.9 x 10^6
VIABLFREEZ   95.8 ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   03032009 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) GROWTEMP   37 C
PASCELLNO   3 - 10 x 10^4 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. After trypsinization, centrifuge the cell suspension and remove the trypsin solution completely.
GROWTHRATE   approx. 1.5 days FREEZCONC   1.4 x 10^6
VIABLFREEZ   94.3 ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   02272008 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.#10099, lot 484375) GROWTEMP   37 C
PASCELLNO   3 - 10 x 10^4 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA
GROWTHRATE   30 hrs FREEZCONC   2.2 x 10^6
VIABLFREEZ   97 ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   07112007 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) [Do not use Fungizone (Amphotericin B). It may result retardation of growth.] GROWTEMP   37 C
PASCELLNO   approx. 1 x 10^5 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA
GROWTHRATE   approx. 2-3 days FREEZCONC   2.3 x 10^6
VIABLFREEZ   98.3 ANTIBIOTIC   free
PASSAGE   P47 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   confirmed as human by NP, G6PD (type B), AST, LD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   07112005 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) GROWTEMP   37 C
PASCELLNO   4 - 9 x 10^4 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA
GROWTHRATE   approx. 2 days FREEZCONC   1.9 x 10^6
VIABLFREEZ   97.2 ANTIBIOTIC   free
PASSAGE   P47 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), LD
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   10022003 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) GROWTEMP   37 C
PASCELLNO   5-10 x 10^4 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA
GROWTHRATE   D.T. = ca. 2 days FREEZCONC   1.8 x 10^6
VIABLFREEZ   90.5 ANTIBIOTIC   free
PASSAGE   P47 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), AST, LD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   03272002 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum GROWTEMP   37 C
PASCELLNO   5-10 x 10^4 cells/ml PASMETHOD   0.2% trypsin and 0.02% EDTA in Ca, Mg-free PBS
GROWTHRATE   D.T. = ca 50 hrs FREEZCONC   9.3 x 10^5
VIABLFREEZ   97 ANTIBIOTIC   free
PASSAGE   P47 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FCS-DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   03292000 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal calf serum GROWTEMP   37 C
PASCELLNO   3.0 x 10^5 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA
GROWTHRATE   Slow FREEZCONC   3.8 x 10^6
VIABLFREEZ    ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), AST.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD    EXOGENE   
FMEDIUM   10% DMSO, 20% FCS-DMEM CO2   
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   102296 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum (JRS DO10400) GROWTEMP   37 C
PASCELLNO   2.2 x 10^5 cells/ml PASMETHOD   Wash cells with PBS(-) once and treat cells with 0.1% trypsin and 0.02% EDTA for 5 min. at 37 C.
GROWTHRATE   NT FREEZCONC   1.7x10^6
VIABLFREEZ   91.0 ANTIBIOTIC   free
PASSAGE   P49 PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   G6PD(type B), LDH, NP. (human)
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM    CO2   
RFLP    FROMLOT   021296
Additional information   


Images
Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   021396 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum. GROWTEMP   37 C
PASCELLNO   2.5x10^4 cells/ml PASMETHOD   Cells rinsed with PBS(-) and treated with 0.1% trypsin and 0.005% EDTA at 37 C.
GROWTHRATE   NT FREEZCONC   3.9x10^6
VIABLFREEZ   80.2 ANTIBIOTIC   free
PASSAGE   P44 PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   NT
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM    CO2   
RFLP    FROMLOT   102287
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   122597 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 6M0121) GROWTEMP   37 C
PASCELLNO   5-10 x 10^4 cells/ml PASMETHOD   0.2% trypsin and 0.02% EDTA
GROWTHRATE   about 4 days FREEZCONC   1.1 x 10^6
VIABLFREEZ   95.0 ANTIBIOTIC   free
PASSAGE   P55 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM    CO2   
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   
LOT No.   052599 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 6M0121) GROWTEMP   37 C
PASCELLNO   1.0 x 10^5 cells/ml PASMETHOD   0.2% trypsin and 0.02% EDTA
GROWTHRATE   D.T.=ca. 55 hrs FREEZCONC   7.5 x 10^5
VIABLFREEZ   90.0 ANTIBIOTIC   free
PASSAGE   P55 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, MD, LD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD    EXOGENE   
FMEDIUM    CO2   
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   04232013 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). GROWTEMP   37 C
PASCELLNO   5 - 10 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.(ロット番号誤表記:02232013と記載, Ampoule mislabel: lot# 02232013)
GROWTHRATE    FREEZCONC   1.9 x 10^6
VIABLFREEZ   94.6 ANTIBIOTIC   free
PASSAGE   P47 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   10312014 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. Use T25 flask or smaller one (larger flask will carry a risk of low viability) GROWTEMP   37 C
PASCELLNO   approx. 6 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
GROWTHRATE    FREEZCONC   2.6 x 10^6
VIABLFREEZ   87.5 ANTIBIOTIC   free
PASSAGE   P47 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   
Additional information   


Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   06222015 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. GROWTEMP   37 C
PASCELLNO   4.8 - 7.8 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
GROWTHRATE   approx. 2.5 days FREEZCONC   1.2 x 10^6
VIABLFREEZ   95 ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS -DMEM CO2   5%
RFLP    FROMLOT   021296
Additional information   


Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   04182016 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. GROWTEMP   
PASCELLNO   5 - 7 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.2% trypsin and 0.02% EDTA.
GROWTHRATE   approx. 1.5 day FREEZCONC   1.9 x 106
VIABLFREEZ   90.7 ANTIBIOTIC   free
PASSAGE   P47 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   021296
Additional information   


Cell No.   JCRB0403 Cell Name   HuH-7
LOT No.   07222016 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10091). DMEM must contain L-glutamine. DO NOT use high glucose DMEM. GROWTEMP   37 C
PASCELLNO   5.7 - 6.4 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
GROWTHRATE   approx. 52 hrs. FREEZCONC   1.1 x 10^6
VIABLFREEZ   92.7 ANTIBIOTIC   free
PASSAGE   P48 PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   021296
Additional information   


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