AyuK10D10 Ppp2r2d-K1

Cell information

Cell type：mousehomozygous mutant ES cells (View Pricing Information)

JCRB No.   AyuK10D10 Cell Name   Ppp2r2d-K1
Profile   Homozygous mutant mouse embryonic stem cell. Deficient gene: Ppp2r2d Other Name   K10D10
Animal   mouse Strain   (C57BL/6 x 129S4/SvJae)F1
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   Ppp2r2d, protein phosphatase 2, regulatory subunit B, delta isoform
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   pCMT-SAhygpA-NP21
Classify   Homozygous mutant Established by   Horie, K.
Registered by   Horie, K. Regulation for Distribution   
Comment    Year   
Medium   Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells. Methods for Passages   0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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LOT Information

Cell No.   AyuK10D10 Cell Name   Ppp2r2d-K1
LOT No.   12022016 Lot Specification   distribution
Medium   Knockout DMEM with 20% fetal bovine serum(BioWest Lot.S09336S1820,non HI),2mM glutamine,NEAA,sodium pyruvate,0.1mM 2-mercaptoethanol and 1000U/ml LIF on mitomycin C-treated MEF feeder cells. Temperature   37 C
Cell Density at Seeding   0.7-2.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.125%trypsin(GIBCO) and 0.01% EDTA.(1/5-1/10 split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   penicillin:100 units/ml , streptomycin:100 ug/ml
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Mitomycin C-treated MEFs are used as feeders. 1vial is 0.5ml.Type of ampoules is plastic.
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