AyuK19F03 Nmt2-K1 Cell information
Cell type：mousehomozygous mutant ES cells
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JCRB No.
AyuK19F03
Cell Name
Nmt2-K1
Profile
Homozygous mutant mouse embryonic stem cell. Deficient gene: Nmt2
Other Name
K19F03
Animal
mouse
Strain
(C57BL/6 x 129S4/SvJae)F1
Genus
Mus
Species
musclus
Sex
Age
Identity
not done
Tissue for Primary Cancer
embryo
Case history
Metastasis
Tissue Metastasized
Genetics
Nmt2, N-myristoyltransferase 2
Life Span
infinite
Crisis PDL
Morphology
ES-like
Character
pT2F2-SAhygpA-NP21
Classify
Homozygous mutant
Established by
Horie, K.
Registered by
Horie, K.
Regulation for Distribution
Comment
Year
Medium
Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells.
Methods for Passages
0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish
Cell Number on Passage
Race
CO2 Conc.
5%
Tissue Sampling
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
LOT Information
Cell No.
AyuK19F03
Cell Name
Nmt2-K1
LOT No.
10112012
Lot Specification
distribution
Medium
Knockout DMEM with 20% fetal bovine serum(BioWest Lot.S09336S1820,non HI),2mM glutamine,NEAA,sodium pyruvate,0.1mM 2-mercaptoethanol and 1000U/ml LIF on mitomycin C-treated MEF feeder cells.
Temperature
37 C
Cell Density at Seeding
0.8-8.0x10^5 cells/sq.cm
Methods for Passages
Cells were harvested after treatment with 0.125%trypsin(GIBCO) and 0.01% EDTA.(1/5-1/10 split)
Doubling Time
NT
Cell Number in Vial (cells/1ml)
2.06x10^6/0.5 ml
Viability at cell freezing (%)
97.0
Antibiotics Used
penicillin:100 units/ml , streptomycin:100/ug/ml
Passage Number
p3*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
Bambanker(Lymphotec Inc.)
CO2 Conc.
5 %
Viability immediately after thawing (%)
Additional information
