AyuK10C05 Map2k1-K1
Cell information
Cell type:mousehomozygous mutant ES cells (View Pricing Information)
JCRB No. | AyuK10C05 | Cell Name | Map2k1-K1 |
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Profile | Homozygous mutant mouse embryonic stem cell. Deficient gene: Map2k1 | Other Name | K10C05 |
Animal | mouse | Strain | (C57BL/6 x 129S4/SvJae)F1 |
Genus | Mus | Species | musclus |
Sex | Age | ||
Identity | not done | Tissue for Primary Cancer | embryo |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | Map2k1, mitogen activated protein kinase kinase 1 | |
Life Span | infinite | Crisis PDL | |
Morphology | ES-like | Character | pCMT-SAhygpA-NP21 |
Classify | Homozygous mutant | Established by | Horie, K. |
Registered by | Horie, K. | Regulation for Distribution | |
Comment | Year | ||
Medium | Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells. | Methods for Passages | 0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish |
Cell Number on Passage | Race | ||
CO2 Conc. | 5% | Tissue Sampling | |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | not tested | Detected RNA Virus | not tested |