AyuK16C12 Trim71-K1
Cell information
Cell type：mousehomozygous mutant ES cells (View Pricing Information)
| JCRB No. | AyuK16C12 | Cell Name | Trim71-K1 |
|---|---|---|---|
| Profile | Homozygous mutant mouse embryonic stem cell. Deficient gene: Trim71 | Other Name | K16C12 |
| Animal | mouse | Strain | (C57BL/6 x 129S4/SvJae)F1 |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | embryo |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Trim71, tripartite motif-containing 71 | |
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | pCMT-SAhygpA-NP22 |
| Classify | Homozygous mutant | Established by | Horie, K. |
| Registered by | Horie, K. | Regulation for Distribution | |
| Comment | Year | ||
| Medium | Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells. | Methods for Passages | 0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
| Cell No. | AyuK16C12 | Cell Name | Trim71-K1 |
|---|---|---|---|
| LOT No. | 10032014 | Lot Specification | distribution |
| Medium | Temperature | 37 C | |
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | PDL | NT | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | NT | Additional information |
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