AyuK7D01 Cfdp1-K1

Cell information

Cell type：mousehomozygous mutant ES cells (View Pricing Information)

JCRB No.   AyuK7D01 Cell Name   Cfdp1-K1
Profile   Homozygous mutant mouse embryonic stem cell. Deficient gene: Cfdp1 Other Name   K7D01
Animal   mouse Strain   (C57BL/6 x 129S4/SvJae)F1
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   Cfdp1, craniofacial development protein 1
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   pCMT-SAhygpA-NP21
Classify   Homozygous mutant Established by   Horie, K.
Registered by   Horie, K. Regulation for Distribution   
Comment   According to the user's analysis, AyuK7D01 is not "double knockout" for Cfdp1, but Cfdp1 is knocked down. Refer to PubMed ID 32432658. Year   
Medium   Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells. Methods for Passages   0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested


Research results by users. Click！
Pubmed id:32432658 Overcoming off-targets: assessing Western blot signals for Bcnt/Cfdp1, a tentative component of the chromatin remodeling complex.
Iwashita S,Suzuki T,Kiriyama Y,Dohmae N,Ohoka Y,Song SY,Nakashima K
Biosci Rep. 2020 Jun 26;40(6):

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LOT Information

Cell No.   AyuK7D01 Cell Name   Cfdp1-K1
LOT No.   09092016 Lot Specification   distribution
Medium   Knockout DMEM with 20% fetal bovine serum(BioWest Lot.S09336S1820,non HI),2mM glutamine,NEAA,sodium pyruvate,0.1mM 2-mercaptoethanol and 1000U/ml LIF on mitomycin C-treated MEF feeder cells. Temperature   37 C
Cell Density at Seeding   0.5-4.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.125%trypsin(GIBCO) and 0.01% EDTA.(1/5-1/10 split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.60x10^6
Viability at cell freezing (%)   96.9 Antibiotics Used   penicillin:100 units/ml , streptomycin:100 ug/ml
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Mitomycin C-treated MEFs are used as feeders. 1vial is 0.5ml.Type of ampoules is plastic.
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