AyuK7G10 Zfp280b-K1 Cell information
Cell type：mousehomozygous mutant ES cells
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JCRB No.
AyuK7G10
Cell Name
Zfp280b-K1
Profile
Homozygous mutant mouse embryonic stem cell. Deficient gene: Zfp280b
Other Name
K7G10
Animal
mouse
Strain
(C57BL/6 x 129S4/SvJae)F1
Genus
Mus
Species
musclus
Sex
Age
Identity
not done
Tissue for Primary Cancer
embryo
Case history
Metastasis
Tissue Metastasized
Genetics
Zfp280b, zinc finger protein 280b
Life Span
infinite
Crisis PDL
Morphology
ES-like
Character
pCMT-SAhygpA-NP21
Classify
Homozygous mutant
Established by
Horie, K.
Registered by
Horie, K.
Regulation for Distribution
Comment
Year
Medium
Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells.
Methods for Passages
0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish
Cell Number on Passage
Race
CO2 Conc.
5%
Tissue Sampling
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
LOT Information
Cell No.
AyuK7G10
Cell Name
Zfp280b-K1
LOT No.
02262016
Lot Specification
distribution
Medium
Knockout DMEM with 20% fetal bovine serum(BioWest Lot.S09336S1820,non HI),2mM glutamine,NEAA,sodium pyruvate,0.1mM 2-mercaptoethanol and 1000U/ml LIF on mitomycin C-treated MEF feeder cells.
Temperature
37 C
Cell Density at Seeding
0.4-5.0x10^5 cells/sq.cm
Methods for Passages
Cells were harvested after treatment with 0.125%trypsin(GIBCO) and 0.01% EDTA.(1/5-1/10 split)
Doubling Time
NT
Cell Number in Vial (cells/1ml)
NT
Viability at cell freezing (%)
NT
Antibiotics Used
penicillin:100 units/ml , streptomycin:100 ug/ml
Passage Number
p3*
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)
CO2 Conc.
5 %
Viability immediately after thawing (%)
NT
Additional information
Mitomycin C-treated MEFs are used as feeders.
1vial is 0.5ml.
Type of ampoules is plastic.
