IFO50008 Mm1 Cell information
Cell type:general cells
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JCRB No.
IFO50008
Cell Name
Mm1
Profile
spontaneous myeloid leukemia, M-1-derivative, macrophage-like phenotype, (SL mouse)
Other Name
Animal
mouse
Strain
SL
Genus
Mus
Species
musclus
Sex
Age
Identity
Tissue for Primary Cancer
hemo-lymphocytic
Case history
spontaneous myeloid leukemia
Metastasis
Tissue Metastasized
Genetics
subline of M-1 mouse leukemia cell line
Life Span
infinite
Crisis PDL
Morphology
macrophage-like
Character
spontaneous myeloid leukemia, M-1-derivative, macrophage-like phenotype
Classify
tumor
Established by
Maeda, M. & Ichikawa, Y.
Registered by
Tanida,S.
Regulation for Distribution
Comment
Year
1985
Medium
Eagle's minimal essential medium with 10% fetal bovine serum
Methods for Passages
Cultures are cooled at 4 C for a few minutes, and then scraped by a gentle pipetting.
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
bone marrow
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:4517230 Spontaneous development of macrophage-like cells in a culture of myeloid leukemia cells. Maeda M,Ichikawa Y Gan. 1973 Jun;64(3):265-71
Images
LOT Information
Cell No.
IFO50008
Cell Name
Mm1
LOT No.
1063
Lot Specification
distribution
Medium
E.MEM, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
0.025% trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
7 x 10^5
Viability at cell freezing (%)
84
Antibiotics Used
free
Passage Number
Unknown (6 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, G6PD, PepB
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Lot# is labeled as R17F1063 on vials.