IFO50019 NIH 3T3 p-7 cl-3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50019 | Cell Name | NIH 3T3 p-7 cl-3 |
|---|---|---|---|
| Profile | fibroblast (NIH Swiss mouse) | Other Name | |
| Animal | mouse | Strain | NIH Swiss |
| Genus | Mus | Species | musclus |
| Sex | Age | embryo | |
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | fibroblasts from embryonic mouse. |
| Classify | normal | Established by | Hakura, A. |
| Registered by | Hakura,A. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | embryo, whole |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:4087463 | Two NIH3T3 cell lines of different origins circulating in the world. Iwamoto A,Masuda M,Yoshikura H Jpn J Exp Med. 1985 Jun;55(3):129-31 |
| Pubmed id:3918269 | Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells. Mulcahy LS,Smith MR,Stacey DW Nature. 1985 Jan 17-23;313(5999):241-3 |
| Pubmed id:742018 | [Therapeutic results in adult acute leukemia in Giessen]. Löffler H,Pralle H,Graubner M,Schrezenmaier E Verh Dtsch Ges Inn Med. 1978;(84):156-8 |
| Pubmed id:4311790 | Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. Jainchill JL,Aaronson SA,Todaro GJ J Virol. 1969 Nov;4(5):549-53 |
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LOT Information
| Cell No. | IFO50019 | Cell Name | |
|---|---|---|---|
| LOT No. | 01252011 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | approx. 3.6 x 10^4 | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to full confluency. |
| Doubling Time | approx. 1 day | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
| Viability at cell freezing (%) | 96.7 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50019 | Cell Name | NIH 3T3 p-7 cl-3 |
|---|---|---|---|
| LOT No. | 344 | Lot Specification | distribution |
| Medium | E.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2 x 10^6 | |
| Viability at cell freezing (%) | 85 | Antibiotics Used | free |
| Passage Number | Unknown (>3 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50019 | Cell Name | NIH 3T3 p-7 cl-3 |
|---|---|---|---|
| LOT No. | 340 | Lot Specification | distribution |
| Medium | E.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2 x 10^6 | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unknown (>3 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP,MD,AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50019 | Cell Name | NIH 3T3 p-7 cl-3 |
|---|---|---|---|
| LOT No. | 11072022 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 19 hrs. | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | Unknown (3 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 82 | Additional information |