IFO50046 Raji Cell information
Cell type:general cells
(View Pricing Information )
JCRB No.
IFO50046
Cell Name
Raji
Profile
lymphoma, Burkitt's
Other Name
Animal
human
Strain
Genus
Homo
Species
sapiens
Sex
M
Age
11
Identity
available
Tissue for Primary Cancer
hemo-lymphocytic
Case history
Burkitt's lymphoma
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
lymphoblast-like
Character
lymphoma, Burkitt's
Classify
tumor
Established by
Pulvertaft, R. J. V.
Registered by
Takeuchi,M.
Regulation for Distribution
Comment
Year
1985
Medium
RPMI1640 medium with 10% fetal bovine serum
Methods for Passages
dilution
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
maxilla
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
tested
HCV
-
HTLV-1
-
HIV-1
-
HTLV-2
-
HIV-2
-
HAV
-
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
Notes
LOT Information
Cell No.
IFO50046
Cell Name
Raji
LOT No.
1155
Lot Specification
distribution
Medium
RPMI 1640, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
1 x 10^5 cells/ml
Methods for Passages
dilution
Doubling Time
Cell Number in Vial (cells/1ml)
2.3 x 10^6
Viability at cell freezing (%)
90
Antibiotics Used
free
Passage Number
Unknown (5 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, G6PD, LD
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.)-culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
IFO50046
Cell Name
Raji
LOT No.
88
Lot Specification
distribution
Medium
RPMI 1640, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
dilution
Doubling Time
Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)
Antibiotics Used
free
Passage Number
Unknown (6 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, G6PD(type B), MD, MPI
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
IFO50046
Cell Name
Raji
LOT No.
11262015
Lot Specification
distribution
Medium
RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO cat. # 10091)
Temperature
37 C
Cell Density at Seeding
5.8 - 7.2 x 10^5 cells/mL
Methods for Passages
Simple dilution (suspension culture). Cells grow with forming cell aggregates.
Doubling Time
37 C
Cell Number in Vial (cells/1ml)
3.6 x 10^6
Viability at cell freezing (%)
83.4
Antibiotics Used
free
Passage Number
Unknown (6 at bank)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
No
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI1640
CO2 Conc.
5%
Viability immediately after thawing (%)
83.4
Additional information