IFO50100 F2408-B993

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50100 Cell Name   F2408-B993
Profile   HGPRT-deficient line from F2408 (IFO 50015). This cell line exhibits temperature sensitive phenotype for transformation by Moloney MSV. Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   HGPRT(-), temperature sensitive when transformed by MSV.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   HGPRT-deficient cell line from F2408. This cell line exhibits temperature sensitive phenotype for transformation by Moloney MSV. The transforming efficiency considerably decreases at 39 C compared to at 33 C.
Classify   mutant Established by   Inoue, H.
Registered by   Inoue,H. Regulation for Distribution   
Comment    Year   1986
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryonic fibroblast
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_E952
Images



LOT Information

Cell No.   IFO50100 Cell Name   F2408-B993
LOT No.   1137 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (>2 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
CLOSE