IFO50110 C6 Cell information
Cell type:general cells
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JCRB No.
IFO50110
Cell Name
C6
Profile
glial tumor, GFAP-positive
Other Name
Animal
rat
Strain
Genus
Rattus
Species
norvegicus
Sex
Age
Identity
Tissue for Primary Cancer
neural
Case history
glial tumor
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
fibroblast-like
Character
glial tumor, GFAP-positive
Classify
tumor
Established by
Benda, P. et al.
Registered by
Suno,M.
Regulation for Distribution
Comment
Year
1986
Medium
Ham's F10 medium with 2.5% fetal bovine serum and 10% horse serum
Methods for Passages
trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
brain
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:4873531 Differentiated rat glial cell strain in tissue culture. Benda P,Lightbody J,Sato G,Levine L,Sweet W Science. 1968 Jul 26;161(3839):370-1
Images
LOT Information
Cell No.
IFO50110
Cell Name
LOT No.
07242006
Lot Specification
distribution
Medium
Ham's F10 medium with 2.5% fetal bovine serum and 10% horse seum
Temperature
37 C
Cell Density at Seeding
1-3 x 10^5 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
2.4 x 10^6
Viability at cell freezing (%)
97.9
Antibiotics Used
free
Passage Number
P61
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as rat by NP, G6PD, AST.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - Ham's F10
CO2 Conc.
5%
Viability immediately after thawing (%)
Additional information
Cell No.
IFO50110
Cell Name
C6
LOT No.
993
Lot Specification
distribution
Medium
Ham's F10, 87.5% ; FBS, 2.5% ; horse serum, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
2 x 10^6
Viability at cell freezing (%)
90
Antibiotics Used
free
Passage Number
P62
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, G6PD, PepB
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information