IFO50120 TaD-1-3 Cell information
Cell type:general cells
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JCRB No.
IFO50120
Cell Name
TaD-1-3
Profile
This cell line is a clone of TaD-1 cells (IFO 50112).
Other Name
Animal
rat
Strain
ACI x BUF/Mna, F1
Genus
Rattus
Species
norvegicus
Sex
M
Age
2
Identity
Tissue for Primary Cancer
thymus
Case history
thymoma
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
thymoma, keratin-positive
Classify
tumor
Established by
Masuda, A.
Registered by
Masuda,A.
Regulation for Distribution
Comment
Year
1987
Medium
Eagle's minimal essential medium with 10% fetal bovine serum and 1 uM dexamethasone
Methods for Passages
trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
thymus
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:2384449 Establishment of functional epithelial cell lines from a rat thymoma and a rat thymus. Masuda A,Ohtsuka K,Matsuyama M In Vitro Cell Dev Biol. 1990 Jul;26(7):713-21
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LOT Information
Cell No.
IFO50120
Cell Name
TaD-1-3
LOT No.
453
Lot Specification
distribution
Medium
E.MEM, 90% ; FBS, 10% ; 1uM dexamethasone
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
5 x 10^5
Viability at cell freezing (%)
85
Antibiotics Used
free
Passage Number
Unknown (5 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, G6PD, MD, AST, LD
Chromosome Mode
80
Chromosome Information
77-160
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information