IFO50270 6-23 clone 6 Cell information
Cell type:general cells
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JCRB No.
IFO50270
Cell Name
6-23 clone 6
Profile
medullary thyroid carcinoma, neurotensin and calcitonin-producing, (WAG/RIJ rat)
Other Name
Animal
rat
Strain
WAG/RIJ
Genus
Rattus
Species
norvegicus
Sex
Age
Identity
Tissue for Primary Cancer
thyroid
Case history
medually thyroid carcinoma
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
round-bi/tripolar
Character
medullary thyroid carcinoma, neurotensin and calcitonin-producing
Classify
tumor
Established by
Zeytinoglu, F. N. & Gagel, R. F.
Registered by
Igarashi,K.
Regulation for Distribution
Comment
Year
1990
Medium
Dulbecco's modified Eagle's medium with 10% horse serum
Methods for Passages
trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
thyroid, C cell
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:6248326 Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. I. Morphological studies of the tumor and cells in culture. Zeytinoğlu FN,DeLellis RA,Gagel RF,Wolfe HJ,Tashjian AH Jr Endocrinology. 1980 Aug;107(2):509-15
Images
LOT Information
Cell No.
IFO50270
Cell Name
6-23 clone 6
LOT No.
1028
Lot Specification
distribution
Medium
D.MEM, 90% ; horse serum, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
4 x 10^6
Viability at cell freezing (%)
95
Antibiotics Used
free
Passage Number
Unknown (7 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information